Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish
Summary: The whole kidney marrow (WKM) is the site for hematopoiesis in the adult zebrafish. Here, we present a protocol for analyzing hematopoietic lineages in the WKM of adult zebrafish. We describe steps for the isolation of hematopoietic cells from the WKM, the downstream analysis of total marro...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723007773 |
| _version_ | 1797347771733245952 |
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| author | Christopher B. Mahony Rui Monteiro |
| author_facet | Christopher B. Mahony Rui Monteiro |
| author_sort | Christopher B. Mahony |
| collection | DOAJ |
| description | Summary: The whole kidney marrow (WKM) is the site for hematopoiesis in the adult zebrafish. Here, we present a protocol for analyzing hematopoietic lineages in the WKM of adult zebrafish. We describe steps for the isolation of hematopoietic cells from the WKM, the downstream analysis of total marrow cellularity, and analysis of cell populations by flow cytometry. We then detail procedures for May-Grünwald-Giemsa staining for analysis of cellular morphology and phenotyping.For complete details on the use and execution of this protocol, please refer to Mahony et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-08T11:53:34Z |
| format | Article |
| id | doaj.art-ada7826ca6f64427ae6c87e4a8baec66 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-08T11:53:34Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-ada7826ca6f64427ae6c87e4a8baec662024-01-24T05:21:53ZengElsevierSTAR Protocols2666-16672024-03-0151102810Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafishChristopher B. Mahony0Rui Monteiro1Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK; Corresponding authorInstitute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK; Corresponding authorSummary: The whole kidney marrow (WKM) is the site for hematopoiesis in the adult zebrafish. Here, we present a protocol for analyzing hematopoietic lineages in the WKM of adult zebrafish. We describe steps for the isolation of hematopoietic cells from the WKM, the downstream analysis of total marrow cellularity, and analysis of cell populations by flow cytometry. We then detail procedures for May-Grünwald-Giemsa staining for analysis of cellular morphology and phenotyping.For complete details on the use and execution of this protocol, please refer to Mahony et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723007773Cell isolationSingle CellFlow CytometryModel OrganismsGene Expression |
| spellingShingle | Christopher B. Mahony Rui Monteiro Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish STAR Protocols Cell isolation Single Cell Flow Cytometry Model Organisms Gene Expression |
| title | Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish |
| title_full | Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish |
| title_fullStr | Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish |
| title_full_unstemmed | Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish |
| title_short | Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish |
| title_sort | protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish |
| topic | Cell isolation Single Cell Flow Cytometry Model Organisms Gene Expression |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723007773 |
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