Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization
One promising research trend involves evaluating the influence of microbiota in the reproductive system of women on becoming pregnant and maintaining pregnancy. The goal of this study was to define the microflora profile of the endometrium and uterine cervix in women qualified for an in vitro fertil...
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MDPI AG
2022-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/12/3348 |
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| author | Anna Bednarska-Czerwińska Michał Czerwiński Emilia Morawiec Aleksandra Łach Anna Ziaja Adrian Kusaj Patrycja Strączyńska Dorota Sagan Dariusz Boroń Beniamin Oskar Grabarek |
| author_facet | Anna Bednarska-Czerwińska Michał Czerwiński Emilia Morawiec Aleksandra Łach Anna Ziaja Adrian Kusaj Patrycja Strączyńska Dorota Sagan Dariusz Boroń Beniamin Oskar Grabarek |
| author_sort | Anna Bednarska-Czerwińska |
| collection | DOAJ |
| description | One promising research trend involves evaluating the influence of microbiota in the reproductive system of women on becoming pregnant and maintaining pregnancy. The goal of this study was to define the microflora profile of the endometrium and uterine cervix in women qualified for an in vitro fertilization (IVF) procedure, which is expected to contribute to increasing the percentage of successful IVF implantations. Based on the conducted molecular analysis in the collected swabs, 22 bacterial strains were identified. Eleven strains (57%) that were isolated belong to the physiological microflora, the most common strain of which was Lactobacillus. Eight of the isolated strains (33%) were pathological microflora, among which the most common bacteria were from the Enterobacteriaceae family (which includes <i>E. coli</i>, Shigella, and Salmonella). Finally, three of the bacterial strains (10%) may be a component of both physiological or pathological microflora of the vagina: <i>Bifidobacterium breve</i>, <i>Bifidobacterium longum</i> group, and <i>Alloscardovia omnicolens</i>. The presence of <i>Escherichia coli</i> was detected in six women, <i>Staphylococcus aureus</i> also in six patients, <i>Atopobium parvulum</i> in three, <i>Streptococcus salivarius</i> group in three, <i>Enterococcus faecalis</i> in four, and <i>Aerococcus christensenii</i> in two patients. We found statistically significant relationships (<i>p</i> < 0.05) between <i>Lactobacillus fermentum</i> and <i>Enterococcus faecalis</i>, <i>Lactobacillus delbrueckii</i> and <i>Escherichia coli</i> groups, <i>Lactobacillus FN667084_s</i> and <i>Staphylococcus aureus</i> groups, as well as <i>Lactobacillus fermentum</i> and <i>Streptococcus agalactiae</i>. Based on the conducted study, it may be confirmed that the endometrium is, to a large extent, colonized by lactic acid bacilli. Apart from that, endometrial dysbiosis was not noted in patients qualified for the IVF procedure. |
| first_indexed | 2024-03-09T23:28:31Z |
| format | Article |
| id | doaj.art-adac10271f4d4ab9bd9e53b415f0196f |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T23:28:31Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
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| series | Journal of Clinical Medicine |
| spelling | doaj.art-adac10271f4d4ab9bd9e53b415f0196f2023-11-23T17:14:26ZengMDPI AGJournal of Clinical Medicine2077-03832022-06-011112334810.3390/jcm11123348Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro FertilizationAnna Bednarska-Czerwińska0Michał Czerwiński1Emilia Morawiec2Aleksandra Łach3Anna Ziaja4Adrian Kusaj5Patrycja Strączyńska6Dorota Sagan7Dariusz Boroń8Beniamin Oskar Grabarek9Gyncentrum Fertility Clinic, 40-121 Katowice, PolandGyncentrum Fertility Clinic, 40-121 Katowice, PolandGyncentrum Fertility Clinic, 40-121 Katowice, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, PolandGyncentrum Fertility Clinic, 40-121 Katowice, PolandMedical Center Dormed Medical SPA, 28-105 Busko-Zdroj, PolandDepartment of Histology, Cytophysiology and Embryology, Faculty of Medicine, University of Technology, Academy of Silesia in Katowice, 41-800 Zabrze, PolandGyncentrum Fertility Clinic, 40-121 Katowice, PolandOne promising research trend involves evaluating the influence of microbiota in the reproductive system of women on becoming pregnant and maintaining pregnancy. The goal of this study was to define the microflora profile of the endometrium and uterine cervix in women qualified for an in vitro fertilization (IVF) procedure, which is expected to contribute to increasing the percentage of successful IVF implantations. Based on the conducted molecular analysis in the collected swabs, 22 bacterial strains were identified. Eleven strains (57%) that were isolated belong to the physiological microflora, the most common strain of which was Lactobacillus. Eight of the isolated strains (33%) were pathological microflora, among which the most common bacteria were from the Enterobacteriaceae family (which includes <i>E. coli</i>, Shigella, and Salmonella). Finally, three of the bacterial strains (10%) may be a component of both physiological or pathological microflora of the vagina: <i>Bifidobacterium breve</i>, <i>Bifidobacterium longum</i> group, and <i>Alloscardovia omnicolens</i>. The presence of <i>Escherichia coli</i> was detected in six women, <i>Staphylococcus aureus</i> also in six patients, <i>Atopobium parvulum</i> in three, <i>Streptococcus salivarius</i> group in three, <i>Enterococcus faecalis</i> in four, and <i>Aerococcus christensenii</i> in two patients. We found statistically significant relationships (<i>p</i> < 0.05) between <i>Lactobacillus fermentum</i> and <i>Enterococcus faecalis</i>, <i>Lactobacillus delbrueckii</i> and <i>Escherichia coli</i> groups, <i>Lactobacillus FN667084_s</i> and <i>Staphylococcus aureus</i> groups, as well as <i>Lactobacillus fermentum</i> and <i>Streptococcus agalactiae</i>. Based on the conducted study, it may be confirmed that the endometrium is, to a large extent, colonized by lactic acid bacilli. Apart from that, endometrial dysbiosis was not noted in patients qualified for the IVF procedure.https://www.mdpi.com/2077-0383/11/12/3348microbiomeendometriummicrobiotauterine cervixin vitro fertilizationnext-generation sequencing |
| spellingShingle | Anna Bednarska-Czerwińska Michał Czerwiński Emilia Morawiec Aleksandra Łach Anna Ziaja Adrian Kusaj Patrycja Strączyńska Dorota Sagan Dariusz Boroń Beniamin Oskar Grabarek Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization Journal of Clinical Medicine microbiome endometrium microbiota uterine cervix in vitro fertilization next-generation sequencing |
| title | Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization |
| title_full | Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization |
| title_fullStr | Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization |
| title_full_unstemmed | Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization |
| title_short | Marking the Profile of the Microflora of the Endometrium and Uterine Cervix in Women as a Potential Factor Determining the Effectiveness of In Vitro Fertilization |
| title_sort | marking the profile of the microflora of the endometrium and uterine cervix in women as a potential factor determining the effectiveness of in vitro fertilization |
| topic | microbiome endometrium microbiota uterine cervix in vitro fertilization next-generation sequencing |
| url | https://www.mdpi.com/2077-0383/11/12/3348 |
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