Expression of isg15 in bone marrow during early pregnancy in ewes
Interferon-tau (IFNT) is the main signal for maternal recognition of pregnancy in ruminants. IFNT acts on the endometrium, corpus luteum, and liver through paracrine and endocrine style, which is involved in inhibiting the development of luteolytic mechanism and suppressing maternal immune rejection...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2017-04-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2109 |
| _version_ | 1797800586533404672 |
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| author | Ling YANG Baoliang LIU Xianxi YAN Leying ZHANG Feng GAO Zhichao LIU |
| author_facet | Ling YANG Baoliang LIU Xianxi YAN Leying ZHANG Feng GAO Zhichao LIU |
| author_sort | Ling YANG |
| collection | DOAJ |
| description | Interferon-tau (IFNT) is the main signal for maternal recognition of pregnancy in ruminants. IFNT acts on the endometrium, corpus luteum,
and liver through paracrine and endocrine style, which is involved in inhibiting the development of luteolytic mechanism and suppressing
maternal immune rejection of the semi-allogeneic fetus. Bone marrow (BM) is a key component of the lymphatic system, supports the body"s
immune system through producing the lymphocytes. At present study, the BM was obtained from days 13, 16 and 25 of pregnant ewes, day
16 of non-pregnant ewes to study the expression of interferon stimulated gene 15 kDa protein (ISG15) mRNA and protein through a qRT-PCR
assay, Western blot, and an immunohistochemistry analysis. Our results showed that the expression of ISG15 mRNA, proteins and conjugated
proteins were up-regulated in the stroma of BM during early pregnancy, and the immunohistochemistry results confirmed that the ISG15
proteins were localized in the cytoplasm of different cells in the stroma of BM. In conclusion, IFNT derived from the conceptus induced upregulated
expression of ISG15 and conjugated proteins in the stroma of BM through an endocrine style, which were involved in regulating the
maternal immune response during early pregnancy in ewes. |
| first_indexed | 2024-03-13T04:37:36Z |
| format | Article |
| id | doaj.art-adacba75fcb64d0da3ea3ea704dbc529 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:37:36Z |
| publishDate | 2017-04-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-adacba75fcb64d0da3ea3ea704dbc5292023-06-19T06:45:16ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512017-04-0123576777210.9775/kvfd.2017.177262109Expression of isg15 in bone marrow during early pregnancy in ewesLing YANG0Baoliang LIU1Xianxi YAN2Leying ZHANG3Feng GAO4Zhichao LIU5Department of Animal Science, College of Life Science and Food Engineering, Hebei University of Engineering, Handan, CHINADepartment of Animal Science, College of Life Science and Food Engineering, Hebei University of Engineering, Handan, CHINADepartment of Animal Science, College of Life Science and Food Engineering, Hebei University of Engineering, Handan, CHINADepartment of Animal Science, College of Life Science and Food Engineering, Hebei University of Engineering, Handan, CHINADepartment of Animal Science, College of Life Science and Food Engineering, Hebei University of Engineering, Handan, CHINADepartment of Animal Science, College of Life Science and Food Engineering, Hebei University of Engineering, Handan, CHINAInterferon-tau (IFNT) is the main signal for maternal recognition of pregnancy in ruminants. IFNT acts on the endometrium, corpus luteum, and liver through paracrine and endocrine style, which is involved in inhibiting the development of luteolytic mechanism and suppressing maternal immune rejection of the semi-allogeneic fetus. Bone marrow (BM) is a key component of the lymphatic system, supports the body"s immune system through producing the lymphocytes. At present study, the BM was obtained from days 13, 16 and 25 of pregnant ewes, day 16 of non-pregnant ewes to study the expression of interferon stimulated gene 15 kDa protein (ISG15) mRNA and protein through a qRT-PCR assay, Western blot, and an immunohistochemistry analysis. Our results showed that the expression of ISG15 mRNA, proteins and conjugated proteins were up-regulated in the stroma of BM during early pregnancy, and the immunohistochemistry results confirmed that the ISG15 proteins were localized in the cytoplasm of different cells in the stroma of BM. In conclusion, IFNT derived from the conceptus induced upregulated expression of ISG15 and conjugated proteins in the stroma of BM through an endocrine style, which were involved in regulating the maternal immune response during early pregnancy in ewes.https://vetdergikafkas.org/pdf.php?id=2109ewesbone marrowpregnancyinterferon stimulated gene 15 kda protein |
| spellingShingle | Ling YANG Baoliang LIU Xianxi YAN Leying ZHANG Feng GAO Zhichao LIU Expression of isg15 in bone marrow during early pregnancy in ewes Kafkas Universitesi Veteriner Fakültesi Dergisi ewes bone marrow pregnancy interferon stimulated gene 15 kda protein |
| title | Expression of isg15 in bone marrow during early pregnancy in ewes |
| title_full | Expression of isg15 in bone marrow during early pregnancy in ewes |
| title_fullStr | Expression of isg15 in bone marrow during early pregnancy in ewes |
| title_full_unstemmed | Expression of isg15 in bone marrow during early pregnancy in ewes |
| title_short | Expression of isg15 in bone marrow during early pregnancy in ewes |
| title_sort | expression of isg15 in bone marrow during early pregnancy in ewes |
| topic | ewes bone marrow pregnancy interferon stimulated gene 15 kda protein |
| url | https://vetdergikafkas.org/pdf.php?id=2109 |
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