Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food
The observed increase in the prevalence of gluten-related disorders has prompted the development of novel immunological systems for gluten detection in foodstuff. The innovation on these methods relies on the generation of new antibodies, which might alternatively be obtained by molecular evolution...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/1/149 |
| _version_ | 1797625774202683392 |
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| author | Eduardo Garcia-Calvo Aina García-García Santiago Rodríguez Sergio Farrais Rosario Martín Teresa García |
| author_facet | Eduardo Garcia-Calvo Aina García-García Santiago Rodríguez Sergio Farrais Rosario Martín Teresa García |
| author_sort | Eduardo Garcia-Calvo |
| collection | DOAJ |
| description | The observed increase in the prevalence of gluten-related disorders has prompted the development of novel immunological systems for gluten detection in foodstuff. The innovation on these methods relies on the generation of new antibodies, which might alternatively be obtained by molecular evolution methods such as phage display. This work presents a novel approach for the generation of a Fab library by merging semi-synthetic heavy chains built-up from a pre-existent recombinant antibody fragment (dAb8E) with an immune light chain set derived from celiac donors. From the initial phage population (10<sup>7</sup> candidates) and after three rounds of selection and amplification, four different clones were isolated for further characterization. The phage Fab8E-4 presented the best features to be applied in an indirect ELISA for the detection of gluten in foods, resulting in improved specificity and sensitivity. |
| first_indexed | 2024-03-11T10:01:07Z |
| format | Article |
| id | doaj.art-adb010540bdc49b9b5aebcdb6b408af9 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T10:01:07Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-adb010540bdc49b9b5aebcdb6b408af92023-11-16T15:23:04ZengMDPI AGFoods2304-81582022-12-0112114910.3390/foods12010149Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in FoodEduardo Garcia-Calvo0Aina García-García1Santiago Rodríguez2Sergio Farrais3Rosario Martín4Teresa García5Departamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainServicio de Medicina Digestiva, Hospital Universitario Fundación Jiménez Díaz, 28040 Madrid, SpainDepartamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Nutrición y Ciencia de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, SpainThe observed increase in the prevalence of gluten-related disorders has prompted the development of novel immunological systems for gluten detection in foodstuff. The innovation on these methods relies on the generation of new antibodies, which might alternatively be obtained by molecular evolution methods such as phage display. This work presents a novel approach for the generation of a Fab library by merging semi-synthetic heavy chains built-up from a pre-existent recombinant antibody fragment (dAb8E) with an immune light chain set derived from celiac donors. From the initial phage population (10<sup>7</sup> candidates) and after three rounds of selection and amplification, four different clones were isolated for further characterization. The phage Fab8E-4 presented the best features to be applied in an indirect ELISA for the detection of gluten in foods, resulting in improved specificity and sensitivity.https://www.mdpi.com/2304-8158/12/1/149glutenprolaminsceliac diseasephage displayELISAimmune library |
| spellingShingle | Eduardo Garcia-Calvo Aina García-García Santiago Rodríguez Sergio Farrais Rosario Martín Teresa García Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food Foods gluten prolamins celiac disease phage display ELISA immune library |
| title | Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food |
| title_full | Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food |
| title_fullStr | Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food |
| title_full_unstemmed | Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food |
| title_short | Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food |
| title_sort | construction of a fab library merging chains from semisynthetic and immune origin suitable for developing new tools for gluten immunodetection in food |
| topic | gluten prolamins celiac disease phage display ELISA immune library |
| url | https://www.mdpi.com/2304-8158/12/1/149 |
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