Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice
DREAM (downstream regulatory element antagonist modulator) is a Ca2+-binding protein that binds DNA and represses transcription in a Ca2+-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2012-05-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnmol.2012.00058/full |
| _version_ | 1811270932566638592 |
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| author | Mara eDierssen Laura eFedrizzi Rosa eGomez-Villafuertes Maria eMartinez de Lagran Alfonso eGutierrez-Adan Ignaci eSahun Belen ePintado Juan Carlos Oliveros Xose Manuel Dopazo Paz eGonzalez Marisa eBrini Britt eMellstrom Ernesto eCarafoli JOSE R. NARANJO JOSE R. NARANJO |
| author_facet | Mara eDierssen Laura eFedrizzi Rosa eGomez-Villafuertes Maria eMartinez de Lagran Alfonso eGutierrez-Adan Ignaci eSahun Belen ePintado Juan Carlos Oliveros Xose Manuel Dopazo Paz eGonzalez Marisa eBrini Britt eMellstrom Ernesto eCarafoli JOSE R. NARANJO JOSE R. NARANJO |
| author_sort | Mara eDierssen |
| collection | DOAJ |
| description | DREAM (downstream regulatory element antagonist modulator) is a Ca2+-binding protein that binds DNA and represses transcription in a Ca2+-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar granular neurons to control Ca2+ homeostasis and survival of these neurons. To achieve a global view of the genes regulated by DREAM in the cerebellum, we performed a genome-wide analysis in transgenic cerebellum expressing a Ca2+-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). Here we show that DREAM regulates the expression of the midline 1 (Mid1) gene early after birth. As a consequence, daDREAM mice exhibit a significant shortening of the rostro-caudal axis of the cerebellum and a severe delay in neuromotor development early after birth. Our results indicate a role for DREAM in cerebellar function. |
| first_indexed | 2024-04-12T22:11:04Z |
| format | Article |
| id | doaj.art-adb09dce15ce4a518d865dbd09def6ef |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-04-12T22:11:04Z |
| publishDate | 2012-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-adb09dce15ce4a518d865dbd09def6ef2022-12-22T03:14:44ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992012-05-01510.3389/fnmol.2012.0005823431Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic miceMara eDierssen0Laura eFedrizzi1Rosa eGomez-Villafuertes2Maria eMartinez de Lagran3Alfonso eGutierrez-Adan4Ignaci eSahun5Belen ePintado6Juan Carlos Oliveros7Xose Manuel Dopazo8Paz eGonzalez9Marisa eBrini10Britt eMellstrom11Ernesto eCarafoli12JOSE R. NARANJO13JOSE R. NARANJO14CENTRO DE REGULACION GENOMICAUniversity of PaduaCENTRO NALCIONAL DE BIOTECNOLOGIA/CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASCENTRO DE REGULACION GENOMICAINIACENTRO DE REGULACION GENOMICACENTRO NALCIONAL DE BIOTECNOLOGIA/CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASCENTRO NALCIONAL DE BIOTECNOLOGIA/CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASCENTRO NALCIONAL DE BIOTECNOLOGIA/CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASCENTRO NALCIONAL DE BIOTECNOLOGIA/CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASUniversity of PaduaCENTRO NALCIONAL DE BIOTECNOLOGIA/CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASVenetian Institute of Molecular MedicineCENTRO NALCIONAL DE BIOTECNOLOGIA/CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICASCIBERNED-CNBDREAM (downstream regulatory element antagonist modulator) is a Ca2+-binding protein that binds DNA and represses transcription in a Ca2+-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar granular neurons to control Ca2+ homeostasis and survival of these neurons. To achieve a global view of the genes regulated by DREAM in the cerebellum, we performed a genome-wide analysis in transgenic cerebellum expressing a Ca2+-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). Here we show that DREAM regulates the expression of the midline 1 (Mid1) gene early after birth. As a consequence, daDREAM mice exhibit a significant shortening of the rostro-caudal axis of the cerebellum and a severe delay in neuromotor development early after birth. Our results indicate a role for DREAM in cerebellar function.http://journal.frontiersin.org/Journal/10.3389/fnmol.2012.00058/fullCerebellumTransgenic miceCa2+-dependent gene expressiondaDREAMGenome-wide analysisMidline 1 gene |
| spellingShingle | Mara eDierssen Laura eFedrizzi Rosa eGomez-Villafuertes Maria eMartinez de Lagran Alfonso eGutierrez-Adan Ignaci eSahun Belen ePintado Juan Carlos Oliveros Xose Manuel Dopazo Paz eGonzalez Marisa eBrini Britt eMellstrom Ernesto eCarafoli JOSE R. NARANJO JOSE R. NARANJO Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice Frontiers in Molecular Neuroscience Cerebellum Transgenic mice Ca2+-dependent gene expression daDREAM Genome-wide analysis Midline 1 gene |
| title | Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice |
| title_full | Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice |
| title_fullStr | Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice |
| title_full_unstemmed | Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice |
| title_short | Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice |
| title_sort | reduced mid1 expression and delayed neuromotor development in dadream transgenic mice |
| topic | Cerebellum Transgenic mice Ca2+-dependent gene expression daDREAM Genome-wide analysis Midline 1 gene |
| url | http://journal.frontiersin.org/Journal/10.3389/fnmol.2012.00058/full |
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