Development and Evaluation of Vero Cell-Derived Master Donor Viruses for Influenza Pandemic Preparedness
The embryonated egg-based platform currently produces the majority of seasonal influenza vaccines by employing a well-developed master donor virus (MDV, A/PR/8/34 (PR8)) to generate high-growth reassortants (HGRs) for A/H1N1 and A/H3N2 subtypes. Although the egg-based platform can supply enough seas...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/8/4/626 |
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| author | Po-Ling Chen Tsai-Teng Tzeng Alan Yung-Chih Hu Lily Hui-Ching Wang Min-Shi Lee |
| author_facet | Po-Ling Chen Tsai-Teng Tzeng Alan Yung-Chih Hu Lily Hui-Ching Wang Min-Shi Lee |
| author_sort | Po-Ling Chen |
| collection | DOAJ |
| description | The embryonated egg-based platform currently produces the majority of seasonal influenza vaccines by employing a well-developed master donor virus (MDV, A/PR/8/34 (PR8)) to generate high-growth reassortants (HGRs) for A/H1N1 and A/H3N2 subtypes. Although the egg-based platform can supply enough seasonal influenza vaccines, it cannot meet surging demands during influenza pandemics. Therefore, multi-purpose platforms are desirable for pandemic preparedness. The Vero cell-based production platform is widely used for human vaccines and could be a potential multi-purpose platform for pandemic influenza vaccines. However, many wild-type and egg-derived influenza viruses cannot grow efficiently in Vero cells. Therefore, it is critical to develop Vero cell-derived high-growth MDVs for pandemic preparedness. In this study, we evaluated two in-house MDVs (Vero-15 and VB5) and two external MDVs (PR8 and PR8-HY) to generate Vero cell-derived HGRs for five avian influenza viruses (AIVs) with pandemic potentials (H5N1 clade 2.3.4, H5N1 clade 2.3.2.1, American-lineage H5N2, H7N9 first wave and H7N9 fifth wave). Overall, no single MDV could generate HGRs for all five AIVs, but this goal could be achieved by employing two in-house MDVs (vB5 and Vero-15). In immunization studies, mice received two doses of Vero cell-derived inactivated H5N1 and H7N9 whole virus antigens adjuvanted with alum and developed robust antibody responses. |
| first_indexed | 2024-03-10T15:20:58Z |
| format | Article |
| id | doaj.art-adb51df2af734a539fdadd5598f15150 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T15:20:58Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-adb51df2af734a539fdadd5598f151502023-11-20T18:29:27ZengMDPI AGVaccines2076-393X2020-10-018462610.3390/vaccines8040626Development and Evaluation of Vero Cell-Derived Master Donor Viruses for Influenza Pandemic PreparednessPo-Ling Chen0Tsai-Teng Tzeng1Alan Yung-Chih Hu2Lily Hui-Ching Wang3Min-Shi Lee4National Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, TaiwanNational Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, TaiwanNational Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, TaiwanInstitute of Molecular and Cellular Biology, National Tsing Hua University, Hsinchu 300044, TaiwanNational Institution of Infectious Diseases and Vaccinology, National Health Research Institutes (NHRI), Zhunan, Miaoli 35053, TaiwanThe embryonated egg-based platform currently produces the majority of seasonal influenza vaccines by employing a well-developed master donor virus (MDV, A/PR/8/34 (PR8)) to generate high-growth reassortants (HGRs) for A/H1N1 and A/H3N2 subtypes. Although the egg-based platform can supply enough seasonal influenza vaccines, it cannot meet surging demands during influenza pandemics. Therefore, multi-purpose platforms are desirable for pandemic preparedness. The Vero cell-based production platform is widely used for human vaccines and could be a potential multi-purpose platform for pandemic influenza vaccines. However, many wild-type and egg-derived influenza viruses cannot grow efficiently in Vero cells. Therefore, it is critical to develop Vero cell-derived high-growth MDVs for pandemic preparedness. In this study, we evaluated two in-house MDVs (Vero-15 and VB5) and two external MDVs (PR8 and PR8-HY) to generate Vero cell-derived HGRs for five avian influenza viruses (AIVs) with pandemic potentials (H5N1 clade 2.3.4, H5N1 clade 2.3.2.1, American-lineage H5N2, H7N9 first wave and H7N9 fifth wave). Overall, no single MDV could generate HGRs for all five AIVs, but this goal could be achieved by employing two in-house MDVs (vB5 and Vero-15). In immunization studies, mice received two doses of Vero cell-derived inactivated H5N1 and H7N9 whole virus antigens adjuvanted with alum and developed robust antibody responses.https://www.mdpi.com/2076-393X/8/4/626pandemic preparednessVero cell-derived influenza vaccinemaster donor virus |
| spellingShingle | Po-Ling Chen Tsai-Teng Tzeng Alan Yung-Chih Hu Lily Hui-Ching Wang Min-Shi Lee Development and Evaluation of Vero Cell-Derived Master Donor Viruses for Influenza Pandemic Preparedness Vaccines pandemic preparedness Vero cell-derived influenza vaccine master donor virus |
| title | Development and Evaluation of Vero Cell-Derived Master Donor Viruses for Influenza Pandemic Preparedness |
| title_full | Development and Evaluation of Vero Cell-Derived Master Donor Viruses for Influenza Pandemic Preparedness |
| title_fullStr | Development and Evaluation of Vero Cell-Derived Master Donor Viruses for Influenza Pandemic Preparedness |
| title_full_unstemmed | Development and Evaluation of Vero Cell-Derived Master Donor Viruses for Influenza Pandemic Preparedness |
| title_short | Development and Evaluation of Vero Cell-Derived Master Donor Viruses for Influenza Pandemic Preparedness |
| title_sort | development and evaluation of vero cell derived master donor viruses for influenza pandemic preparedness |
| topic | pandemic preparedness Vero cell-derived influenza vaccine master donor virus |
| url | https://www.mdpi.com/2076-393X/8/4/626 |
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