Electrophoretic Deposition of Calcium Phosphates on Carbon–Carbon Composite Implants: Morphology, Phase/Chemical Composition and Biological Reactions
Despite a long period of application of metal implants, carbon–carbon medical composites are also widely used for bone defect prosthesis in surgery, dentistry, and oncology. Such implants might demonstrate excellent mechanical properties, but their biocompatibility and integration efficiency into th...
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MDPI AG
2024-03-01
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| author | Andrei S. Skriabin Petr A. Tsygankov Vladimir R. Vesnin Alexey V. Shakurov Elizaveta S. Skriabina Irina K. Sviridova Natalia S. Sergeeva Valentina A. Kirsanova Suraya A. Akhmedova Victoria V. Zherdeva Yulia S. Lukina Leonid L. Bionyshev-Abramov |
| author_facet | Andrei S. Skriabin Petr A. Tsygankov Vladimir R. Vesnin Alexey V. Shakurov Elizaveta S. Skriabina Irina K. Sviridova Natalia S. Sergeeva Valentina A. Kirsanova Suraya A. Akhmedova Victoria V. Zherdeva Yulia S. Lukina Leonid L. Bionyshev-Abramov |
| author_sort | Andrei S. Skriabin |
| collection | DOAJ |
| description | Despite a long period of application of metal implants, carbon–carbon medical composites are also widely used for bone defect prosthesis in surgery, dentistry, and oncology. Such implants might demonstrate excellent mechanical properties, but their biocompatibility and integration efficiency into the host should be improved. As a method of enhancing, the electrophoretic deposition of fine-dispersed hydroxyapatite (HAp) on porous carbon substrates might be recommended. With electron microscopy, energy dispersion X-ray and Raman spectroscopy, and X-ray diffraction, we found that the deposition and subsequent heat post-treatment (up to the temperature of 400 °C for 1 h) did not lead to any significant phase and chemical transformations of raw non-stoichometric HAp. The Ca/P ratio was ≈1.51 in the coatings. Their non-toxicity, cyto- and biocompatibility were confirmed by in vitro and in vivo studies and no adverse reactions and side effects had been detected in the test. The proposed coating and subsequent heat treatment procedures provided improved biological responses in terms of resorption and biocompatibility had been confirmed by histological, magnetic resonance and X-ray tomographic ex vivo studies on the resected implant-containing biopsy samples from the BDF1 mouse model. The obtained results are expected to be useful for modern medical material science and clinical applications. |
| first_indexed | 2024-04-24T18:11:37Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-04-24T18:11:37Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-adb5790796b64f399582c073617aa4282024-03-27T13:45:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-03-01256337510.3390/ijms25063375Electrophoretic Deposition of Calcium Phosphates on Carbon–Carbon Composite Implants: Morphology, Phase/Chemical Composition and Biological ReactionsAndrei S. Skriabin0Petr A. Tsygankov1Vladimir R. Vesnin2Alexey V. Shakurov3Elizaveta S. Skriabina4Irina K. Sviridova5Natalia S. Sergeeva6Valentina A. Kirsanova7Suraya A. Akhmedova8Victoria V. Zherdeva9Yulia S. Lukina10Leonid L. Bionyshev-Abramov11Department of Power Engineering, Bauman Moscow State Technical University, Moscow 105005, RussiaSchool of Physics, Industrial University of Santander, Bucaramanga 680002, ColombiaDepartment of Power Engineering, Bauman Moscow State Technical University, Moscow 105005, RussiaDepartment of Power Engineering, Bauman Moscow State Technical University, Moscow 105005, RussiaDepartment of Power Engineering, Bauman Moscow State Technical University, Moscow 105005, RussiaP.A. Herzen Moscow Research Oncology Institute, Branch of FSBI “National Medical Research Radiological Centre”, Ministry of Health of the Russian Federation, Moscow 125284, RussiaP.A. Herzen Moscow Research Oncology Institute, Branch of FSBI “National Medical Research Radiological Centre”, Ministry of Health of the Russian Federation, Moscow 125284, RussiaP.A. Herzen Moscow Research Oncology Institute, Branch of FSBI “National Medical Research Radiological Centre”, Ministry of Health of the Russian Federation, Moscow 125284, RussiaP.A. Herzen Moscow Research Oncology Institute, Branch of FSBI “National Medical Research Radiological Centre”, Ministry of Health of the Russian Federation, Moscow 125284, RussiaBach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow 119071, RussiaDepartment of Power Engineering, Bauman Moscow State Technical University, Moscow 105005, RussiaPriorov Central Institute for Trauma and Orthopedics, Moscow 127299, RussiaDespite a long period of application of metal implants, carbon–carbon medical composites are also widely used for bone defect prosthesis in surgery, dentistry, and oncology. Such implants might demonstrate excellent mechanical properties, but their biocompatibility and integration efficiency into the host should be improved. As a method of enhancing, the electrophoretic deposition of fine-dispersed hydroxyapatite (HAp) on porous carbon substrates might be recommended. With electron microscopy, energy dispersion X-ray and Raman spectroscopy, and X-ray diffraction, we found that the deposition and subsequent heat post-treatment (up to the temperature of 400 °C for 1 h) did not lead to any significant phase and chemical transformations of raw non-stoichometric HAp. The Ca/P ratio was ≈1.51 in the coatings. Their non-toxicity, cyto- and biocompatibility were confirmed by in vitro and in vivo studies and no adverse reactions and side effects had been detected in the test. The proposed coating and subsequent heat treatment procedures provided improved biological responses in terms of resorption and biocompatibility had been confirmed by histological, magnetic resonance and X-ray tomographic ex vivo studies on the resected implant-containing biopsy samples from the BDF1 mouse model. The obtained results are expected to be useful for modern medical material science and clinical applications.https://www.mdpi.com/1422-0067/25/6/3375carbon–carbon medical compositeshydroxyapatite coatingselectrophoretic depositionin vitro and in vivo testsradiology studies |
| spellingShingle | Andrei S. Skriabin Petr A. Tsygankov Vladimir R. Vesnin Alexey V. Shakurov Elizaveta S. Skriabina Irina K. Sviridova Natalia S. Sergeeva Valentina A. Kirsanova Suraya A. Akhmedova Victoria V. Zherdeva Yulia S. Lukina Leonid L. Bionyshev-Abramov Electrophoretic Deposition of Calcium Phosphates on Carbon–Carbon Composite Implants: Morphology, Phase/Chemical Composition and Biological Reactions International Journal of Molecular Sciences carbon–carbon medical composites hydroxyapatite coatings electrophoretic deposition in vitro and in vivo tests radiology studies |
| title | Electrophoretic Deposition of Calcium Phosphates on Carbon–Carbon Composite Implants: Morphology, Phase/Chemical Composition and Biological Reactions |
| title_full | Electrophoretic Deposition of Calcium Phosphates on Carbon–Carbon Composite Implants: Morphology, Phase/Chemical Composition and Biological Reactions |
| title_fullStr | Electrophoretic Deposition of Calcium Phosphates on Carbon–Carbon Composite Implants: Morphology, Phase/Chemical Composition and Biological Reactions |
| title_full_unstemmed | Electrophoretic Deposition of Calcium Phosphates on Carbon–Carbon Composite Implants: Morphology, Phase/Chemical Composition and Biological Reactions |
| title_short | Electrophoretic Deposition of Calcium Phosphates on Carbon–Carbon Composite Implants: Morphology, Phase/Chemical Composition and Biological Reactions |
| title_sort | electrophoretic deposition of calcium phosphates on carbon carbon composite implants morphology phase chemical composition and biological reactions |
| topic | carbon–carbon medical composites hydroxyapatite coatings electrophoretic deposition in vitro and in vivo tests radiology studies |
| url | https://www.mdpi.com/1422-0067/25/6/3375 |
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