Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex
Rehabilitative exercise following a brain stroke has beneficial effects on the morphological plasticity of neurons. Particularly, voluntary running exercise after focal cerebral ischemia promotes functional recovery and ameliorates ischemia-induced dendritic spine loss in the peri-infarct motor cort...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | IBRO Neuroscience Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667242123000143 |
| _version_ | 1797804548934336512 |
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| author | Natsumi Yamaguchi Toshinori Sawano Jin Nakatani Akiko Nakano-Doi Takayuki Nakagomi Tomohiro Matsuyama Hidekazu Tanaka |
| author_facet | Natsumi Yamaguchi Toshinori Sawano Jin Nakatani Akiko Nakano-Doi Takayuki Nakagomi Tomohiro Matsuyama Hidekazu Tanaka |
| author_sort | Natsumi Yamaguchi |
| collection | DOAJ |
| description | Rehabilitative exercise following a brain stroke has beneficial effects on the morphological plasticity of neurons. Particularly, voluntary running exercise after focal cerebral ischemia promotes functional recovery and ameliorates ischemia-induced dendritic spine loss in the peri-infarct motor cortex layer 5. Moreover, neuronal morphology is affected by changes in the perineuronal environment. Glial cells, whose phenotypes may be altered by exercise, are known to play a pivotal role in the formation of this perineuronal environment. Herein, we investigated the effects of voluntary running exercise on glial cells after middle cerebral artery occlusion. Voluntary running exercise increased the population of glial fibrillary acidic protein-positive astrocytes born between post-operative days (POD) 0 and 3 on POD15 in the peri-infarct cortex. After exercise, transcriptomic analysis of post-ischemic astrocytes revealed 10 upregulated and 70 downregulated genes. Furthermore, gene ontology analysis showed that the 70 downregulated genes were significantly associated with neuronal morphology. In addition, exercise reduced the number of astrocytes expressing lipocalin 2, a regulator of dendritic spine density, on POD15. Our results suggest that exercise modifies the composition of astrocytic population and their phenotype. |
| first_indexed | 2024-03-13T05:38:51Z |
| format | Article |
| id | doaj.art-adb5e95c45594ce7921eb570726b6be2 |
| institution | Directory Open Access Journal |
| issn | 2667-2421 |
| language | English |
| last_indexed | 2024-03-13T05:38:51Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | IBRO Neuroscience Reports |
| spelling | doaj.art-adb5e95c45594ce7921eb570726b6be22023-06-14T04:34:40ZengElsevierIBRO Neuroscience Reports2667-24212023-06-0114253263Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortexNatsumi Yamaguchi0Toshinori Sawano1Jin Nakatani2Akiko Nakano-Doi3Takayuki Nakagomi4Tomohiro Matsuyama5Hidekazu Tanaka6Pharmacology Laboratory, Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, 1–1-1 Noji-Higashi, Kusatsu, Shiga 525–8577, Japan; Ritsumeikan Advanced Research Academy, 1 Nishinokyo-Suzaku-cho, Nakagyo-ku, Kyoto 604–8520, JapanPharmacology Laboratory, Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, 1–1-1 Noji-Higashi, Kusatsu, Shiga 525–8577, Japan; Corresponding authors.Pharmacology Laboratory, Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, 1–1-1 Noji-Higashi, Kusatsu, Shiga 525–8577, JapanInstitute for Advanced Medical Sciences, Hyogo College of Medicine, 1–1 Mukogawacho, Nishinomiya 663–8501, Japan; Department of Therapeutic Progress in Brain Diseases, Hyogo College of Medicine, 1–1 Mukogawacho, Nishinomiya 663–8501, JapanInstitute for Advanced Medical Sciences, Hyogo College of Medicine, 1–1 Mukogawacho, Nishinomiya 663–8501, Japan; Department of Therapeutic Progress in Brain Diseases, Hyogo College of Medicine, 1–1 Mukogawacho, Nishinomiya 663–8501, JapanDepartment of Therapeutic Progress in Brain Diseases, Hyogo College of Medicine, 1–1 Mukogawacho, Nishinomiya 663–8501, JapanPharmacology Laboratory, Department of Biomedical Sciences, College of Life Sciences, Ritsumeikan University, 1–1-1 Noji-Higashi, Kusatsu, Shiga 525–8577, Japan; Corresponding authors.Rehabilitative exercise following a brain stroke has beneficial effects on the morphological plasticity of neurons. Particularly, voluntary running exercise after focal cerebral ischemia promotes functional recovery and ameliorates ischemia-induced dendritic spine loss in the peri-infarct motor cortex layer 5. Moreover, neuronal morphology is affected by changes in the perineuronal environment. Glial cells, whose phenotypes may be altered by exercise, are known to play a pivotal role in the formation of this perineuronal environment. Herein, we investigated the effects of voluntary running exercise on glial cells after middle cerebral artery occlusion. Voluntary running exercise increased the population of glial fibrillary acidic protein-positive astrocytes born between post-operative days (POD) 0 and 3 on POD15 in the peri-infarct cortex. After exercise, transcriptomic analysis of post-ischemic astrocytes revealed 10 upregulated and 70 downregulated genes. Furthermore, gene ontology analysis showed that the 70 downregulated genes were significantly associated with neuronal morphology. In addition, exercise reduced the number of astrocytes expressing lipocalin 2, a regulator of dendritic spine density, on POD15. Our results suggest that exercise modifies the composition of astrocytic population and their phenotype.http://www.sciencedirect.com/science/article/pii/S2667242123000143Cerebral ischemiaVoluntary running exerciseAstrocytesTranscriptomeProliferation |
| spellingShingle | Natsumi Yamaguchi Toshinori Sawano Jin Nakatani Akiko Nakano-Doi Takayuki Nakagomi Tomohiro Matsuyama Hidekazu Tanaka Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex IBRO Neuroscience Reports Cerebral ischemia Voluntary running exercise Astrocytes Transcriptome Proliferation |
| title | Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex |
| title_full | Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex |
| title_fullStr | Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex |
| title_full_unstemmed | Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex |
| title_short | Voluntary running exercise modifies astrocytic population and features in the peri-infarct cortex |
| title_sort | voluntary running exercise modifies astrocytic population and features in the peri infarct cortex |
| topic | Cerebral ischemia Voluntary running exercise Astrocytes Transcriptome Proliferation |
| url | http://www.sciencedirect.com/science/article/pii/S2667242123000143 |
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