Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells
Thailand's second-leading cause of death is lung cancer. Thai indigenous herbal plants are sought after as an alternative treatment against lung cancer. This work aimed to examine cytotoxic, antiproliferative and antimigratory capacities of different parts of Streblus asper (SA) from northeast...
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| Format: | Article |
| Language: | English |
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AcademicPres
2023-06-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13112 |
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| author | Thipphiya KARIRAT Thitiyakorn PHANITCHA Wanatsuda WIBOONCHAT Worachot SAENGHA Sirirat DEESEENTHUM Issaraporn SOMBOONWATTANAKUL Chadaporn SENAKUN Eakapol WANGKAHART Sirinya PHOLCHAMAT Phitcharat SUNTHAMALA Vijitra LUANG-IN |
| author_facet | Thipphiya KARIRAT Thitiyakorn PHANITCHA Wanatsuda WIBOONCHAT Worachot SAENGHA Sirirat DEESEENTHUM Issaraporn SOMBOONWATTANAKUL Chadaporn SENAKUN Eakapol WANGKAHART Sirinya PHOLCHAMAT Phitcharat SUNTHAMALA Vijitra LUANG-IN |
| author_sort | Thipphiya KARIRAT |
| collection | DOAJ |
| description |
Thailand's second-leading cause of death is lung cancer. Thai indigenous herbal plants are sought after as an alternative treatment against lung cancer. This work aimed to examine cytotoxic, antiproliferative and antimigratory capacities of different parts of Streblus asper (SA) from northeastern Thailand on A549 lung cancer cells. Plant leaves, twigs, bark and wood were used for ethanolic extraction by maceration. The highest cytotoxicity of 85.46% was found in twig extract (IC50 57.46 µg/mL) assessed by an MTT assay. The lowest IC50 (18.42 µg/mL) was also found in twig extract using a clonogenic assay indicating its most antiproliferative activity in a long-term therapy. In addition, all SA extracts displayed antimigratory activity against A549 cells in a dose-dependent fashion, especially twig extract. Apoptotic characteristics were noticeable in SA extract treated cells. The maximum DPPH-scavenging activity, FRAP value, total phenolic and flavonoid content were found in twig extract. GC-MS analysis revealed that twig extract contained four prominent components namely ethyl-α-D-glucopyranoside, hexadecanoic acid, ethyl ester, lupeol and γ-sitosterol. Real-time PCR results showed that genes (Bcl-2, Bax, p21, and cytochrome c) linked to apoptosis were significantly affected by all SA extracts. The various SA components' ethanolic extracts exhibited moderate-to-high cytotoxic action towards A549 cells. This work will significantly advance the utilization of the plant as an alternative source of medicine for rural Thais, and it paves the way for future research to determine the active compound(s) and anticipate new drug candidates.
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| first_indexed | 2024-03-13T02:18:15Z |
| format | Article |
| id | doaj.art-adb770384367413d83c25aaf27f1a19d |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-03-13T02:18:15Z |
| publishDate | 2023-06-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-adb770384367413d83c25aaf27f1a19d2023-06-30T11:05:03ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092023-06-0151210.15835/nbha51213112Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cellsThipphiya KARIRAT0Thitiyakorn PHANITCHA1Wanatsuda WIBOONCHAT2Worachot SAENGHA3Sirirat DEESEENTHUM4Issaraporn SOMBOONWATTANAKUL5Chadaporn SENAKUN6Eakapol WANGKAHART7Sirinya PHOLCHAMAT8Phitcharat SUNTHAMALA9Vijitra LUANG-IN10Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150Mahasarakham University, Walai Rukhavej Botanical Research Institute, Mahasarakham 44150Mahasarakham University, Faculty of Technology, Laboratory of Fish Immunology and Nutrigenomics, Applied Animal and Aquatic Sciences Research Unit, Division of Fisheries, Maha Sarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Maha Sarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Maha Sarakham 44150Mahasarakham University, Faculty of Technology, Department of Biotechnology, Natural Antioxidant Innovation Research Unit, Mahasarakham 44150 Thailand's second-leading cause of death is lung cancer. Thai indigenous herbal plants are sought after as an alternative treatment against lung cancer. This work aimed to examine cytotoxic, antiproliferative and antimigratory capacities of different parts of Streblus asper (SA) from northeastern Thailand on A549 lung cancer cells. Plant leaves, twigs, bark and wood were used for ethanolic extraction by maceration. The highest cytotoxicity of 85.46% was found in twig extract (IC50 57.46 µg/mL) assessed by an MTT assay. The lowest IC50 (18.42 µg/mL) was also found in twig extract using a clonogenic assay indicating its most antiproliferative activity in a long-term therapy. In addition, all SA extracts displayed antimigratory activity against A549 cells in a dose-dependent fashion, especially twig extract. Apoptotic characteristics were noticeable in SA extract treated cells. The maximum DPPH-scavenging activity, FRAP value, total phenolic and flavonoid content were found in twig extract. GC-MS analysis revealed that twig extract contained four prominent components namely ethyl-α-D-glucopyranoside, hexadecanoic acid, ethyl ester, lupeol and γ-sitosterol. Real-time PCR results showed that genes (Bcl-2, Bax, p21, and cytochrome c) linked to apoptosis were significantly affected by all SA extracts. The various SA components' ethanolic extracts exhibited moderate-to-high cytotoxic action towards A549 cells. This work will significantly advance the utilization of the plant as an alternative source of medicine for rural Thais, and it paves the way for future research to determine the active compound(s) and anticipate new drug candidates. https://www.notulaebotanicae.ro/index.php/nbha/article/view/13112A549antiproliferationapoptosiscytotoxicityStreblus asper |
| spellingShingle | Thipphiya KARIRAT Thitiyakorn PHANITCHA Wanatsuda WIBOONCHAT Worachot SAENGHA Sirirat DEESEENTHUM Issaraporn SOMBOONWATTANAKUL Chadaporn SENAKUN Eakapol WANGKAHART Sirinya PHOLCHAMAT Phitcharat SUNTHAMALA Vijitra LUANG-IN Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells Notulae Botanicae Horti Agrobotanici Cluj-Napoca A549 antiproliferation apoptosis cytotoxicity Streblus asper |
| title | Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells |
| title_full | Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells |
| title_fullStr | Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells |
| title_full_unstemmed | Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells |
| title_short | Cytotoxic and antiproliferative effects of Streblus asper from northeastern Thailand on A549 lung cancer cells |
| title_sort | cytotoxic and antiproliferative effects of streblus asper from northeastern thailand on a549 lung cancer cells |
| topic | A549 antiproliferation apoptosis cytotoxicity Streblus asper |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/13112 |
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