Stem cells in end-to-side neurorrhaphy. Experimental study in rats
Abstract Purpose: To evaluate the influence of mesenchymal stem cells from adipose tissue in the end-to-side neurorrhaphy, focusing in the nerve regeneration and the muscle reinnervation in acute trauma. Methods: 140 animals were randomly divided in seven groups: control, denervated, end-to-side n...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2021-01-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020001200206&tlng=en |
| _version_ | 1819106296507400192 |
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| author | Geruza Rezende Paiva Fausto Viterbo Elenice Deffune Maria Aparecida Domingues Custódio |
| author_facet | Geruza Rezende Paiva Fausto Viterbo Elenice Deffune Maria Aparecida Domingues Custódio |
| author_sort | Geruza Rezende Paiva |
| collection | DOAJ |
| description | Abstract Purpose: To evaluate the influence of mesenchymal stem cells from adipose tissue in the end-to-side neurorrhaphy, focusing in the nerve regeneration and the muscle reinnervation in acute trauma. Methods: 140 animals were randomly divided in seven groups: control, denervated, end-to-side neurorrhaphy between distal stump of common peroneal nerve and tibial nerve (ESN), ESN wrapped in fascia, ESN wrapped in fascia and platelet gel, ESN wrapped in platelet gel, ESN wrapped in fascia and platelet gel within stem cells (without culture) removed from the adipose tissue. Mass measurements of the animal and of cranial tibial muscles, electromyography, walking track analysis tests and histological examinations of the nerves and muscles after 180 days was performed. Results: In the groups where the ESN was performed, the results were always better when compared to the denervated group, showing reinnervation in all ESN groups. The most sensitive methods were walking track and histological analysis. Only the group with stem cells showed values similar to the control group, as well as the functional indices of peroneal nerve and the number of nerve fibers in the peroneal nerve. Conclusions: Stem cells were effective in ESN according with the functional index of the peroneal nerve, evaluated by walking track analysis and the number of nerve fibers in the peroneal nerve. |
| first_indexed | 2024-12-22T02:35:53Z |
| format | Article |
| id | doaj.art-adb8ec758a034c65b950f7db232bf5d1 |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-22T02:35:53Z |
| publishDate | 2021-01-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-adb8ec758a034c65b950f7db232bf5d12022-12-21T18:41:46ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502021-01-01351210.1590/acb351207Stem cells in end-to-side neurorrhaphy. Experimental study in ratsGeruza Rezende Paivahttps://orcid.org/0000-0001-8397-8300Fausto Viterbohttps://orcid.org/0000-0001-7282-7566Elenice Deffunehttps://orcid.org/0000-0002-0533-3248Maria Aparecida Domingues Custódiohttps://orcid.org/0000-0002-4023-3410Abstract Purpose: To evaluate the influence of mesenchymal stem cells from adipose tissue in the end-to-side neurorrhaphy, focusing in the nerve regeneration and the muscle reinnervation in acute trauma. Methods: 140 animals were randomly divided in seven groups: control, denervated, end-to-side neurorrhaphy between distal stump of common peroneal nerve and tibial nerve (ESN), ESN wrapped in fascia, ESN wrapped in fascia and platelet gel, ESN wrapped in platelet gel, ESN wrapped in fascia and platelet gel within stem cells (without culture) removed from the adipose tissue. Mass measurements of the animal and of cranial tibial muscles, electromyography, walking track analysis tests and histological examinations of the nerves and muscles after 180 days was performed. Results: In the groups where the ESN was performed, the results were always better when compared to the denervated group, showing reinnervation in all ESN groups. The most sensitive methods were walking track and histological analysis. Only the group with stem cells showed values similar to the control group, as well as the functional indices of peroneal nerve and the number of nerve fibers in the peroneal nerve. Conclusions: Stem cells were effective in ESN according with the functional index of the peroneal nerve, evaluated by walking track analysis and the number of nerve fibers in the peroneal nerve.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020001200206&tlng=enStem CellsMicrosurgeryPeripheral NervesNerve RegenerationRats |
| spellingShingle | Geruza Rezende Paiva Fausto Viterbo Elenice Deffune Maria Aparecida Domingues Custódio Stem cells in end-to-side neurorrhaphy. Experimental study in rats Acta Cirúrgica Brasileira Stem Cells Microsurgery Peripheral Nerves Nerve Regeneration Rats |
| title | Stem cells in end-to-side neurorrhaphy. Experimental study in rats |
| title_full | Stem cells in end-to-side neurorrhaphy. Experimental study in rats |
| title_fullStr | Stem cells in end-to-side neurorrhaphy. Experimental study in rats |
| title_full_unstemmed | Stem cells in end-to-side neurorrhaphy. Experimental study in rats |
| title_short | Stem cells in end-to-side neurorrhaphy. Experimental study in rats |
| title_sort | stem cells in end to side neurorrhaphy experimental study in rats |
| topic | Stem Cells Microsurgery Peripheral Nerves Nerve Regeneration Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502020001200206&tlng=en |
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