Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevis
Lindane is an organochlorine pesticide belonging to persistent organic pollutants (POPs) that has been widely used to treat agricultural pests. It is of particular concern because of its toxicity, persistence and tendency to bioaccumulate in terrestrial and aquatic ecosystems. In this context, we as...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-04-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/15/4/108 |
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| author | Stabili Loredana Pizzolante Graziano Morgante Antonio Nonnis Marzano Carlotta Longo Caterina Aresta Antonella Maria Zambonin Carlo Corriero Giuseppe Alifano Pietro |
| author_facet | Stabili Loredana Pizzolante Graziano Morgante Antonio Nonnis Marzano Carlotta Longo Caterina Aresta Antonella Maria Zambonin Carlo Corriero Giuseppe Alifano Pietro |
| author_sort | Stabili Loredana |
| collection | DOAJ |
| description | Lindane is an organochlorine pesticide belonging to persistent organic pollutants (POPs) that has been widely used to treat agricultural pests. It is of particular concern because of its toxicity, persistence and tendency to bioaccumulate in terrestrial and aquatic ecosystems. In this context, we assessed the role of bacteria associated with the sponge Hymeniacidon perlevis in lindane degradation. Seven bacteria isolates were characterized and identified. These isolates showed a remarkable capacity to utilize lindane as a sole carbon source leading to a percentage of residual lindane ranging from 3% to 13% after 12 days of incubation with the pesticide. The lindane metabolite, 1,3–6-pentachloro-cyclohexene, was identified as result of lindane degradation and determined by gas chromatography–mass spectrometry (GC–MS). The bacteria capable of lindane degradation were identified on the basis of the phenotypic characterization by morphological, biochemical and cultural tests, completed with 16S rDNA sequence analysis, and assigned to Mameliella phaeodactyli, Pseudovibrio ascidiaceicola, Oceanicaulis stylophorae, Ruegeria atlantica and to three new uncharacterized species. The results obtained are a prelude to the development of future strategies for the in situ bioremediation of lindane. |
| first_indexed | 2024-04-13T07:07:58Z |
| format | Article |
| id | doaj.art-adb99243cb024bd3a37fb557e6ee1a0d |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T07:07:58Z |
| publishDate | 2017-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-adb99243cb024bd3a37fb557e6ee1a0d2022-12-22T02:56:56ZengMDPI AGMarine Drugs1660-33972017-04-0115410810.3390/md15040108md15040108Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevisStabili Loredana0Pizzolante Graziano1Morgante Antonio2Nonnis Marzano Carlotta3Longo Caterina4Aresta Antonella Maria5Zambonin Carlo6Corriero Giuseppe7Alifano Pietro8Istituto per l’Ambiente Marino Costiero, Unità Operativa di Supporto di Taranto, CNR, Via Roma 3, 74123 Taranto, ItalyDipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, 73100 Lecce, ItalyDipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, 73100 Lecce, ItalyDipartimento di Biologia, Università di Bari Aldo Moro, 70125 Bari, ItalyDipartimento di Biologia, Università di Bari Aldo Moro, 70125 Bari, ItalyDipartimento di Chimica, Università di Bari Aldo Moro, 70125 Bari, ItalyDipartimento di Chimica, Università di Bari Aldo Moro, 70125 Bari, ItalyDipartimento di Biologia, Università di Bari Aldo Moro, 70125 Bari, ItalyDipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, 73100 Lecce, ItalyLindane is an organochlorine pesticide belonging to persistent organic pollutants (POPs) that has been widely used to treat agricultural pests. It is of particular concern because of its toxicity, persistence and tendency to bioaccumulate in terrestrial and aquatic ecosystems. In this context, we assessed the role of bacteria associated with the sponge Hymeniacidon perlevis in lindane degradation. Seven bacteria isolates were characterized and identified. These isolates showed a remarkable capacity to utilize lindane as a sole carbon source leading to a percentage of residual lindane ranging from 3% to 13% after 12 days of incubation with the pesticide. The lindane metabolite, 1,3–6-pentachloro-cyclohexene, was identified as result of lindane degradation and determined by gas chromatography–mass spectrometry (GC–MS). The bacteria capable of lindane degradation were identified on the basis of the phenotypic characterization by morphological, biochemical and cultural tests, completed with 16S rDNA sequence analysis, and assigned to Mameliella phaeodactyli, Pseudovibrio ascidiaceicola, Oceanicaulis stylophorae, Ruegeria atlantica and to three new uncharacterized species. The results obtained are a prelude to the development of future strategies for the in situ bioremediation of lindane.http://www.mdpi.com/1660-3397/15/4/108H. perlevissponge-associated bacteriagas chromatography–mass spectrometry (GC–MS)lindane1, 3–6-pentachloro-cyclohexenebioremediation |
| spellingShingle | Stabili Loredana Pizzolante Graziano Morgante Antonio Nonnis Marzano Carlotta Longo Caterina Aresta Antonella Maria Zambonin Carlo Corriero Giuseppe Alifano Pietro Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevis Marine Drugs H. perlevis sponge-associated bacteria gas chromatography–mass spectrometry (GC–MS) lindane 1, 3–6-pentachloro-cyclohexene bioremediation |
| title | Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevis |
| title_full | Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevis |
| title_fullStr | Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevis |
| title_full_unstemmed | Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevis |
| title_short | Lindane Bioremediation Capability of Bacteria Associated with the Demosponge Hymeniacidon perlevis |
| title_sort | lindane bioremediation capability of bacteria associated with the demosponge hymeniacidon perlevis |
| topic | H. perlevis sponge-associated bacteria gas chromatography–mass spectrometry (GC–MS) lindane 1, 3–6-pentachloro-cyclohexene bioremediation |
| url | http://www.mdpi.com/1660-3397/15/4/108 |
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