Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?
Abstract Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Metho...
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| Format: | Article |
| Language: | English |
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Federação Brasileira das Sociedades de Ginecologia e Obstetrícia
2019-08-01
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| Series: | Revista Brasileira de Ginecologia e Obstetrícia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019000700449&tlng=en |
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| author | Marcelo Luis Steiner Thérèse Rachell Theodoro Shirley Gimenez Garcia Ana Maria Amaral Antonio Mader Luciano de Melo Pompei Maria Aparecida da Silva Pinhal César Eduardo Fernandes |
| author_facet | Marcelo Luis Steiner Thérèse Rachell Theodoro Shirley Gimenez Garcia Ana Maria Amaral Antonio Mader Luciano de Melo Pompei Maria Aparecida da Silva Pinhal César Eduardo Fernandes |
| author_sort | Marcelo Luis Steiner |
| collection | DOAJ |
| description | Abstract Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by realtime polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries. Results The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups. Conclusion Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats. |
| first_indexed | 2024-04-11T17:20:31Z |
| format | Article |
| id | doaj.art-adba7ee2ceff4f32ae22bce1e4010ed0 |
| institution | Directory Open Access Journal |
| issn | 0100-7203 |
| language | English |
| last_indexed | 2024-04-11T17:20:31Z |
| publishDate | 2019-08-01 |
| publisher | Federação Brasileira das Sociedades de Ginecologia e Obstetrícia |
| record_format | Article |
| series | Revista Brasileira de Ginecologia e Obstetrícia |
| spelling | doaj.art-adba7ee2ceff4f32ae22bce1e4010ed02022-12-22T04:12:31ZengFederação Brasileira das Sociedades de Ginecologia e ObstetríciaRevista Brasileira de Ginecologia e Obstetrícia0100-72032019-08-0141744945310.1055/s-0039-1693681Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone?Marcelo Luis Steinerhttps://orcid.org/0000-0002-2693-9695Thérèse Rachell TheodoroShirley Gimenez GarciaAna Maria Amaral Antonio MaderLuciano de Melo PompeiMaria Aparecida da Silva PinhalCésar Eduardo FernandesAbstract Objective To analyze the effects of estrogen alone or in combination with progestogens and tibolone (TIB) on the expression of the extracellular matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9), of perlecan, and of heparanase (HPSE) of the vascular walls of the carotid arteries. Methods A total of 30 250-day-old ovariectomized Wistar rats were orally treated for 5 weeks with: a) 1 mg/kg of estradiol benzoate (EB); b) EB + 0.2 mg/kg of medroxyprogesterone acetate (MPA); c) EB + 0.2mg/kg of norethisterone acetate (NETA); d) EB + 2 mg/kg of dydrogesterone (DI); e) 1 mg/kg of TIB; f) placebo (CTR). Following treatment, the expression of mRNA for MMP-2, MMP-9, and HPSE was analyzed by realtime polymerase chain-reaction (PCR), and the expression of MMP-2, of MMP-9, of tissue inhibitor of metalloproteinase 2 (TIMP-2), and of perlecan was quantified by immunohistochemistry in the carotid arteries. Results The groups showed significant differences on mRNA HPSE expression (p = 0.048), which was higher in the EB, EB + MPA, and TIB groups. There was no statistically significant difference in mRNA MMP-2 or MMP-9 expression. The immunohistochemical expression of MMP-2, of TIMP-2, of MMP-9, of HPSE, and of perlecan showed no differences between groups. Conclusion Estradiol alone or associated with MPA and TIB treatment can increase mRNA HSPE expression of the walls of the carotid arteries in ovariectomized rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019000700449&tlng=enextracellular matrixproteoglycansatherosclerosisestrogenprogestogentibolone |
| spellingShingle | Marcelo Luis Steiner Thérèse Rachell Theodoro Shirley Gimenez Garcia Ana Maria Amaral Antonio Mader Luciano de Melo Pompei Maria Aparecida da Silva Pinhal César Eduardo Fernandes Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? Revista Brasileira de Ginecologia e Obstetrícia extracellular matrix proteoglycans atherosclerosis estrogen progestogen tibolone |
| title | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
| title_full | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
| title_fullStr | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
| title_full_unstemmed | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
| title_short | Is the Expression of the Components of the Carotid Matrix of Rats Influenced by Estrogen, Progestin and Tibolone? |
| title_sort | is the expression of the components of the carotid matrix of rats influenced by estrogen progestin and tibolone |
| topic | extracellular matrix proteoglycans atherosclerosis estrogen progestogen tibolone |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-72032019000700449&tlng=en |
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