Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration
Objective: San-Cao granule (SCG), a traditional Chinese herb formula, has been used for treating autoimmune hepatitis (AIH) in our clinics for a long time. However, its active ingredients and mechanisms of action were still unknown due to its complicated chemical compositions. In the present study,...
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| Format: | Article |
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Frontiers Media S.A.
2018-06-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2018.00624/full |
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| author | YuXue Yang YuXue Yang Ping Zhang Yingying Wang Yingying Wang Shizhang Wei Lu Zhang Lu Zhang Jiabo Wang Xiaohua Lu Xiaohua Lu Houqin Zhou Houqin Zhou Ruisheng Li Jianxia Wen Jianxia Wen Xuelin Zhou Haotian Li Kun Li Yanling Zhao |
| author_facet | YuXue Yang YuXue Yang Ping Zhang Yingying Wang Yingying Wang Shizhang Wei Lu Zhang Lu Zhang Jiabo Wang Xiaohua Lu Xiaohua Lu Houqin Zhou Houqin Zhou Ruisheng Li Jianxia Wen Jianxia Wen Xuelin Zhou Haotian Li Kun Li Yanling Zhao |
| author_sort | YuXue Yang |
| collection | DOAJ |
| description | Objective: San-Cao granule (SCG), a traditional Chinese herb formula, has been used for treating autoimmune hepatitis (AIH) in our clinics for a long time. However, its active ingredients and mechanisms of action were still unknown due to its complicated chemical compositions. In the present study, the pharmacological study of SCG on acute liver injury induced by Concanavalin A (Con A) was performed to provide a scientific evidence for SCG against liver injury.Methods: In order to screen active components and predicate mechanisms of action, an “ingredients-target-disease” interaction network was constructed by network pharmacology. Then, the pharmacological study was performed to evaluate the therapeutic effect and the underlying mechanisms of SCG on Con A-induced liver injury in mice.Results: This research demonstrated the pharmacological effect of SCG on Con A-induced liver injury, which was through improving the liver function, relieving the pathological changes of liver tissue, decreasing the level of pro-inflammatory cytokines, and thus balancing the pro- and anti-inflammatory cytokines. And the anti-inflammatory of SCG may advantage over the ursodeoxycholic acid (UDCA). Network pharmacology analysis revealed that the pharmacological effect of SCG might be related to its active ingredients of taraxanthin, dihydrotanshinone I, isotanshinone I, γ-sitosterol, 3β-acetyl-20,25-epoxydammarane-24α, and δ-7-stigmastenol. The hepatoprotective effect of SCG was reflected by suppressing Con A-induced apoptosis which was mediated by TRAIL and FASL.Conclusion: The combination of network pharmacology and experimental data has revealed the anti-apoptotic effect of SCG against Con A-induced liver injury. |
| first_indexed | 2024-04-13T17:14:53Z |
| format | Article |
| id | doaj.art-adbc484735384928a8a41dfd4b21d5b9 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-13T17:14:53Z |
| publishDate | 2018-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-adbc484735384928a8a41dfd4b21d5b92022-12-22T02:38:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122018-06-01910.3389/fphar.2018.00624338569Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action ExplorationYuXue Yang0YuXue Yang1Ping Zhang2Yingying Wang3Yingying Wang4Shizhang Wei5Lu Zhang6Lu Zhang7Jiabo Wang8Xiaohua Lu9Xiaohua Lu10Houqin Zhou11Houqin Zhou12Ruisheng Li13Jianxia Wen14Jianxia Wen15Xuelin Zhou16Haotian Li17Kun Li18Yanling Zhao19College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaDepartment of Integrative Medical Center, 302 Military Hospital of China, Beijing, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaDepartment of Integrative Medical Center, 302 Military Hospital of China, Beijing, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaExperimental Laboratory Center, 302 Military Hospital of China, Beijing, ChinaCollege of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaDepartment of Pharmacy, 302 Military Hospital of China, Beijing, ChinaObjective: San-Cao granule (SCG), a traditional Chinese herb formula, has been used for treating autoimmune hepatitis (AIH) in our clinics for a long time. However, its active ingredients and mechanisms of action were still unknown due to its complicated chemical compositions. In the present study, the pharmacological study of SCG on acute liver injury induced by Concanavalin A (Con A) was performed to provide a scientific evidence for SCG against liver injury.Methods: In order to screen active components and predicate mechanisms of action, an “ingredients-target-disease” interaction network was constructed by network pharmacology. Then, the pharmacological study was performed to evaluate the therapeutic effect and the underlying mechanisms of SCG on Con A-induced liver injury in mice.Results: This research demonstrated the pharmacological effect of SCG on Con A-induced liver injury, which was through improving the liver function, relieving the pathological changes of liver tissue, decreasing the level of pro-inflammatory cytokines, and thus balancing the pro- and anti-inflammatory cytokines. And the anti-inflammatory of SCG may advantage over the ursodeoxycholic acid (UDCA). Network pharmacology analysis revealed that the pharmacological effect of SCG might be related to its active ingredients of taraxanthin, dihydrotanshinone I, isotanshinone I, γ-sitosterol, 3β-acetyl-20,25-epoxydammarane-24α, and δ-7-stigmastenol. The hepatoprotective effect of SCG was reflected by suppressing Con A-induced apoptosis which was mediated by TRAIL and FASL.Conclusion: The combination of network pharmacology and experimental data has revealed the anti-apoptotic effect of SCG against Con A-induced liver injury.https://www.frontiersin.org/article/10.3389/fphar.2018.00624/fullSan-Cao granulehepatoprotective effectactive ingredients predictionCon A-induced liver injuryanti-apoptosis |
| spellingShingle | YuXue Yang YuXue Yang Ping Zhang Yingying Wang Yingying Wang Shizhang Wei Lu Zhang Lu Zhang Jiabo Wang Xiaohua Lu Xiaohua Lu Houqin Zhou Houqin Zhou Ruisheng Li Jianxia Wen Jianxia Wen Xuelin Zhou Haotian Li Kun Li Yanling Zhao Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration Frontiers in Pharmacology San-Cao granule hepatoprotective effect active ingredients prediction Con A-induced liver injury anti-apoptosis |
| title | Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration |
| title_full | Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration |
| title_fullStr | Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration |
| title_full_unstemmed | Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration |
| title_short | Hepatoprotective Effect of San-Cao Granule on Con A-Induced Liver Injury in Mice and Mechanisms of Action Exploration |
| title_sort | hepatoprotective effect of san cao granule on con a induced liver injury in mice and mechanisms of action exploration |
| topic | San-Cao granule hepatoprotective effect active ingredients prediction Con A-induced liver injury anti-apoptosis |
| url | https://www.frontiersin.org/article/10.3389/fphar.2018.00624/full |
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