The E46K mutation modulates α-synuclein prion replication in transgenic mice
In multiple system atrophy (MSA), the α-synuclein protein misfolds into a self-templating prion conformation that spreads throughout the brain, leading to progressive neurodegeneration. While the E46K mutation in α-synuclein causes familial Parkinson’s disease (PD), we previously discovered that thi...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-12-01
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| Series: | PLoS Pathogens |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714912/?tool=EBI |
| _version_ | 1811205181199613952 |
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| author | Sara A. M. Holec Jisoo Lee Abby Oehler Lyn Batia Aryanna Wiggins-Gamble Jeffrey Lau Felicia K. Ooi Gregory E. Merz Man Wang Daniel A. Mordes Steven H. Olson Amanda L. Woerman |
| author_facet | Sara A. M. Holec Jisoo Lee Abby Oehler Lyn Batia Aryanna Wiggins-Gamble Jeffrey Lau Felicia K. Ooi Gregory E. Merz Man Wang Daniel A. Mordes Steven H. Olson Amanda L. Woerman |
| author_sort | Sara A. M. Holec |
| collection | DOAJ |
| description | In multiple system atrophy (MSA), the α-synuclein protein misfolds into a self-templating prion conformation that spreads throughout the brain, leading to progressive neurodegeneration. While the E46K mutation in α-synuclein causes familial Parkinson’s disease (PD), we previously discovered that this mutation blocks in vitro propagation of MSA prions. Recent studies by others indicate that α-synuclein adopts a misfolded conformation in MSA in which a Greek key motif is stabilized by an intramolecular salt bridge between residues E46 and K80. Hypothesizing that the E46K mutation impedes salt bridge formation and, therefore, exerts a selective pressure that can modulate α-synuclein strain propagation, we asked whether three distinct α-synuclein prion strains could propagate in TgM47+/- mice, which express human α-synuclein with the E46K mutation. Following intracranial injection of these strains, TgM47+/- mice were resistant to MSA prion transmission, whereas recombinant E46K preformed fibrils (PFFs) transmitted neurological disease to mice and induced the formation of phosphorylated α-synuclein neuropathology. In contrast, heterotypic seeding following wild-type (WT) PFF–inoculation resulted in preclinical α-synuclein prion propagation. Moreover, when we inoculated TgM20+/- mice, which express WT human α-synuclein, with E46K PFFs, we observed delayed transmission kinetics with an incomplete attack rate. These findings suggest that the E46K mutation constrains the number of α-synuclein prion conformations that can propagate in TgM47+/- mice, expanding our understanding of the selective pressures that impact α-synuclein prion replication. Author summary The underlying cause of disease in a group of movement disorders called syncleinopathies is the misfolding of the protein α-synuclein into a shape that self-templates to spread disease. The type of synucleinopathy that a patient develops depends on the shape α-synuclein adopts during misfolding. For example, α-synuclein misfolds into one shape in patients with Parkinson’s disease (PD) and into another in patients with multiple system atrophy (MSA). These distinct forms are referred to as strains. Understanding α-synuclein strain biology, and the factors that impact strain formation and spread, is imperative for developing successful diagnostics and therapeutics. In this study, we investigated the effect of the E46K mutation in α-synuclein, which causes PD, on α-synuclein strain biology to determine how the patient’s genetic background can impact disease. Our results showed that this particular mutation modulates α-synuclein spread; while mice expressing E46K α-synuclein were resistant to MSA prion replication, synthetic E46K fibrils induced neurological disease in the mice. In contrast, heterotypic seeding of wild-type synthetic fibrils yielded inefficient propagation that could not induce neurological signs in the mice. These results indicate that patient genotype can greatly impact α-synuclein strain formation and, therefore, clinical presentation in synucleinopathy patients. |
| first_indexed | 2024-04-12T03:27:27Z |
| format | Article |
| id | doaj.art-adbceadced54473286bcda98c09c99fb |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-12T03:27:27Z |
| publishDate | 2022-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-adbceadced54473286bcda98c09c99fb2022-12-22T03:49:40ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-12-011812The E46K mutation modulates α-synuclein prion replication in transgenic miceSara A. M. HolecJisoo LeeAbby OehlerLyn BatiaAryanna Wiggins-GambleJeffrey LauFelicia K. OoiGregory E. MerzMan WangDaniel A. MordesSteven H. OlsonAmanda L. WoermanIn multiple system atrophy (MSA), the α-synuclein protein misfolds into a self-templating prion conformation that spreads throughout the brain, leading to progressive neurodegeneration. While the E46K mutation in α-synuclein causes familial Parkinson’s disease (PD), we previously discovered that this mutation blocks in vitro propagation of MSA prions. Recent studies by others indicate that α-synuclein adopts a misfolded conformation in MSA in which a Greek key motif is stabilized by an intramolecular salt bridge between residues E46 and K80. Hypothesizing that the E46K mutation impedes salt bridge formation and, therefore, exerts a selective pressure that can modulate α-synuclein strain propagation, we asked whether three distinct α-synuclein prion strains could propagate in TgM47+/- mice, which express human α-synuclein with the E46K mutation. Following intracranial injection of these strains, TgM47+/- mice were resistant to MSA prion transmission, whereas recombinant E46K preformed fibrils (PFFs) transmitted neurological disease to mice and induced the formation of phosphorylated α-synuclein neuropathology. In contrast, heterotypic seeding following wild-type (WT) PFF–inoculation resulted in preclinical α-synuclein prion propagation. Moreover, when we inoculated TgM20+/- mice, which express WT human α-synuclein, with E46K PFFs, we observed delayed transmission kinetics with an incomplete attack rate. These findings suggest that the E46K mutation constrains the number of α-synuclein prion conformations that can propagate in TgM47+/- mice, expanding our understanding of the selective pressures that impact α-synuclein prion replication. Author summary The underlying cause of disease in a group of movement disorders called syncleinopathies is the misfolding of the protein α-synuclein into a shape that self-templates to spread disease. The type of synucleinopathy that a patient develops depends on the shape α-synuclein adopts during misfolding. For example, α-synuclein misfolds into one shape in patients with Parkinson’s disease (PD) and into another in patients with multiple system atrophy (MSA). These distinct forms are referred to as strains. Understanding α-synuclein strain biology, and the factors that impact strain formation and spread, is imperative for developing successful diagnostics and therapeutics. In this study, we investigated the effect of the E46K mutation in α-synuclein, which causes PD, on α-synuclein strain biology to determine how the patient’s genetic background can impact disease. Our results showed that this particular mutation modulates α-synuclein spread; while mice expressing E46K α-synuclein were resistant to MSA prion replication, synthetic E46K fibrils induced neurological disease in the mice. In contrast, heterotypic seeding of wild-type synthetic fibrils yielded inefficient propagation that could not induce neurological signs in the mice. These results indicate that patient genotype can greatly impact α-synuclein strain formation and, therefore, clinical presentation in synucleinopathy patients.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714912/?tool=EBI |
| spellingShingle | Sara A. M. Holec Jisoo Lee Abby Oehler Lyn Batia Aryanna Wiggins-Gamble Jeffrey Lau Felicia K. Ooi Gregory E. Merz Man Wang Daniel A. Mordes Steven H. Olson Amanda L. Woerman The E46K mutation modulates α-synuclein prion replication in transgenic mice PLoS Pathogens |
| title | The E46K mutation modulates α-synuclein prion replication in transgenic mice |
| title_full | The E46K mutation modulates α-synuclein prion replication in transgenic mice |
| title_fullStr | The E46K mutation modulates α-synuclein prion replication in transgenic mice |
| title_full_unstemmed | The E46K mutation modulates α-synuclein prion replication in transgenic mice |
| title_short | The E46K mutation modulates α-synuclein prion replication in transgenic mice |
| title_sort | e46k mutation modulates α synuclein prion replication in transgenic mice |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714912/?tool=EBI |
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