Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations
Abstract Microorganisms play essential roles in the health and resilience of cnidarians. Understanding the factors influencing cnidarian microbiomes requires cross study comparisons, yet the plethora of protocols used hampers dataset integration. We unify 16S rRNA gene sequences from cnidarian micro...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-08-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-39876-6 |
| _version_ | 1797558094864056320 |
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| author | M. McCauley T. L. Goulet C. R. Jackson S. Loesgen |
| author_facet | M. McCauley T. L. Goulet C. R. Jackson S. Loesgen |
| author_sort | M. McCauley |
| collection | DOAJ |
| description | Abstract Microorganisms play essential roles in the health and resilience of cnidarians. Understanding the factors influencing cnidarian microbiomes requires cross study comparisons, yet the plethora of protocols used hampers dataset integration. We unify 16S rRNA gene sequences from cnidarian microbiome studies under a single analysis pipeline. We reprocess 12,010 cnidarian microbiome samples from 186 studies, alongside 3,388 poriferan, 370 seawater samples, and 245 cultured Symbiodiniaceae, unifying ~6.5 billion sequence reads. Samples are partitioned by hypervariable region and sequencing platform to reduce sequencing variability. This systematic review uncovers an incredible diversity of 86 archaeal and bacterial phyla associated with Cnidaria, and highlights key bacteria hosted across host sub-phylum, depth, and microhabitat. Shallow (< 30 m) water Alcyonacea and Actinaria are characterized by highly shared and relatively abundant microbial communities, unlike Scleractinia and most deeper cnidarians. Utilizing the V4 region, we find that cnidarian microbial composition, richness, diversity, and structure are primarily influenced by host phylogeny, sampling depth, and ocean body, followed by microhabitat and sampling date. We identify host and geographical generalist and specific Endozoicomonas clades within Cnidaria and Porifera. This systematic review forms a framework for understanding factors governing cnidarian microbiomes and creates a baseline for assessing stress associated dysbiosis. |
| first_indexed | 2024-03-10T17:26:41Z |
| format | Article |
| id | doaj.art-adbe663df93647868cf342f7fd82c23c |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:26:41Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-adbe663df93647868cf342f7fd82c23c2023-11-20T10:10:23ZengNature PortfolioNature Communications2041-17232023-08-0114111510.1038/s41467-023-39876-6Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associationsM. McCauley0T. L. Goulet1C. R. Jackson2S. Loesgen3Department of Chemistry, Whitney Laboratory for Marine Bioscience, University of FloridaDepartment of Biology, University of MississippiDepartment of Biology, University of MississippiDepartment of Chemistry, Whitney Laboratory for Marine Bioscience, University of FloridaAbstract Microorganisms play essential roles in the health and resilience of cnidarians. Understanding the factors influencing cnidarian microbiomes requires cross study comparisons, yet the plethora of protocols used hampers dataset integration. We unify 16S rRNA gene sequences from cnidarian microbiome studies under a single analysis pipeline. We reprocess 12,010 cnidarian microbiome samples from 186 studies, alongside 3,388 poriferan, 370 seawater samples, and 245 cultured Symbiodiniaceae, unifying ~6.5 billion sequence reads. Samples are partitioned by hypervariable region and sequencing platform to reduce sequencing variability. This systematic review uncovers an incredible diversity of 86 archaeal and bacterial phyla associated with Cnidaria, and highlights key bacteria hosted across host sub-phylum, depth, and microhabitat. Shallow (< 30 m) water Alcyonacea and Actinaria are characterized by highly shared and relatively abundant microbial communities, unlike Scleractinia and most deeper cnidarians. Utilizing the V4 region, we find that cnidarian microbial composition, richness, diversity, and structure are primarily influenced by host phylogeny, sampling depth, and ocean body, followed by microhabitat and sampling date. We identify host and geographical generalist and specific Endozoicomonas clades within Cnidaria and Porifera. This systematic review forms a framework for understanding factors governing cnidarian microbiomes and creates a baseline for assessing stress associated dysbiosis.https://doi.org/10.1038/s41467-023-39876-6 |
| spellingShingle | M. McCauley T. L. Goulet C. R. Jackson S. Loesgen Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations Nature Communications |
| title | Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations |
| title_full | Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations |
| title_fullStr | Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations |
| title_full_unstemmed | Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations |
| title_short | Systematic review of cnidarian microbiomes reveals insights into the structure, specificity, and fidelity of marine associations |
| title_sort | systematic review of cnidarian microbiomes reveals insights into the structure specificity and fidelity of marine associations |
| url | https://doi.org/10.1038/s41467-023-39876-6 |
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