Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in Rabbits
Ochratoxin A (OTA) is second only to aflatoxin in toxicity among mycotoxins. Recent studies have shown that selenomethionine (SeMet) has a protective effect on mycotoxin-induced toxicity. The purpose of this study was to investigate the protective effect and mechanism of SeMet on OTA-induced liver i...
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MDPI AG
2022-09-01
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| Series: | Toxins |
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| author | Ziqiang Zhang Jingyi Xu Xin Zhang Jiajia Wang Hui Xie Yingying Sun Qianwen Zhang Zhaoyang Chang Yumei Liu |
| author_facet | Ziqiang Zhang Jingyi Xu Xin Zhang Jiajia Wang Hui Xie Yingying Sun Qianwen Zhang Zhaoyang Chang Yumei Liu |
| author_sort | Ziqiang Zhang |
| collection | DOAJ |
| description | Ochratoxin A (OTA) is second only to aflatoxin in toxicity among mycotoxins. Recent studies have shown that selenomethionine (SeMet) has a protective effect on mycotoxin-induced toxicity. The purpose of this study was to investigate the protective effect and mechanism of SeMet on OTA-induced liver injury in rabbits. Sixty 35-day-old rabbits with similar body weight were randomly divided into five groups: control group, OTA group (0.2 mg/kg OTA), OTA + 0.2 mg/kg SeMet group, OTA + 0.4 mg/kg SeMet group and OTA + 0.6 mg/kg SeMet group. Rabbits were fed different doses of the SeMet diet for 21 d, and OTA was administered for one week from day 15 (the control group was provided the same dose of NaHCO<sub>3</sub> solution). The results showed that 0.4 mg/kg SeMet could significantly improve the liver injury induced by OTA poisoning. SeMet supplementation can improve the changes in physiological blood indexes caused by OTA poisoning in rabbits and alleviate pathological damage to the rabbit liver. SeMet also increased the activities of SOD, GSH-Px and T-AOC and significantly decreased the contents of ROS, MDA, IL-1β, IL-6 and TNF-α, effectively alleviating the oxidative stress and inflammatory response caused by OTA poisoning. In addition, OTA poisoning inhibits Nrf2 and HO-1 levels, ultimately leading to peroxide reaction, while SeMet activates the Nrf2 signaling pathway and enhances the expression of the HO-1 downstream Nrf2 gene. These results suggest that Se protects the liver from OTA-induced hepatotoxicity by regulating Nrf2/HO-1 expression. |
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| language | English |
| last_indexed | 2024-03-09T22:19:28Z |
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| spelling | doaj.art-adc662faa27d42b29fcbddafedb594ea2023-11-23T19:17:00ZengMDPI AGToxins2072-66512022-09-0114962810.3390/toxins14090628Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in RabbitsZiqiang Zhang0Jingyi Xu1Xin Zhang2Jiajia Wang3Hui Xie4Yingying Sun5Qianwen Zhang6Zhaoyang Chang7Yumei Liu8College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaCollege of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, ChinaOchratoxin A (OTA) is second only to aflatoxin in toxicity among mycotoxins. Recent studies have shown that selenomethionine (SeMet) has a protective effect on mycotoxin-induced toxicity. The purpose of this study was to investigate the protective effect and mechanism of SeMet on OTA-induced liver injury in rabbits. Sixty 35-day-old rabbits with similar body weight were randomly divided into five groups: control group, OTA group (0.2 mg/kg OTA), OTA + 0.2 mg/kg SeMet group, OTA + 0.4 mg/kg SeMet group and OTA + 0.6 mg/kg SeMet group. Rabbits were fed different doses of the SeMet diet for 21 d, and OTA was administered for one week from day 15 (the control group was provided the same dose of NaHCO<sub>3</sub> solution). The results showed that 0.4 mg/kg SeMet could significantly improve the liver injury induced by OTA poisoning. SeMet supplementation can improve the changes in physiological blood indexes caused by OTA poisoning in rabbits and alleviate pathological damage to the rabbit liver. SeMet also increased the activities of SOD, GSH-Px and T-AOC and significantly decreased the contents of ROS, MDA, IL-1β, IL-6 and TNF-α, effectively alleviating the oxidative stress and inflammatory response caused by OTA poisoning. In addition, OTA poisoning inhibits Nrf2 and HO-1 levels, ultimately leading to peroxide reaction, while SeMet activates the Nrf2 signaling pathway and enhances the expression of the HO-1 downstream Nrf2 gene. These results suggest that Se protects the liver from OTA-induced hepatotoxicity by regulating Nrf2/HO-1 expression.https://www.mdpi.com/2072-6651/14/9/628Ochratoxin A (OTA)Nrf2oxidative stressinflammationselenomethionine (SeMet) |
| spellingShingle | Ziqiang Zhang Jingyi Xu Xin Zhang Jiajia Wang Hui Xie Yingying Sun Qianwen Zhang Zhaoyang Chang Yumei Liu Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in Rabbits Toxins Ochratoxin A (OTA) Nrf2 oxidative stress inflammation selenomethionine (SeMet) |
| title | Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in Rabbits |
| title_full | Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in Rabbits |
| title_fullStr | Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in Rabbits |
| title_full_unstemmed | Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in Rabbits |
| title_short | Protective Effect of SeMet on Liver Injury Induced by Ochratoxin A in Rabbits |
| title_sort | protective effect of semet on liver injury induced by ochratoxin a in rabbits |
| topic | Ochratoxin A (OTA) Nrf2 oxidative stress inflammation selenomethionine (SeMet) |
| url | https://www.mdpi.com/2072-6651/14/9/628 |
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