Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress
Calcium-dependent protein kinases (CDPK) are implicated in signaling transduction in eukaryotic organisms. It is largely unknown whether <i>StCDPK28</i> plays a role in the response to water deficiency and osmotic stress in potato plants (<i>Solanum tuberosum</i> L.). Potato...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/15/8795 |
| Summary: | Calcium-dependent protein kinases (CDPK) are implicated in signaling transduction in eukaryotic organisms. It is largely unknown whether <i>StCDPK28</i> plays a role in the response to water deficiency and osmotic stress in potato plants (<i>Solanum tuberosum</i> L.). Potato cv. Zihuabai was cultivated under natural, moderate, and severe water deficiency conditions; to induce osmotic stress, potato plants were treated with 10% or 20% PEG. <i>StCDPK28</i>-overexpression and <i>StCDPK28</i>-knockdown plants were constructed. <i>StCDPKs</i> were evaluated by qRT-PCR. The subcellular location of the StCDPK28 protein was observed with confocal scanning laser microscopy. Phenotypic changes were indicated by photosynthetic activity, the contents of H<sub>2</sub>O<sub>2</sub>, MDA and proline, and the activities of CAT, SOD and POD. Results showed water deficiency and osmotic stress altered <i>StCDPK</i> expression patterns. StCDPK28 exhibited a membrane, cytosolic and nuclear localization. Water deficiency and osmotic stress induced <i>StCDPK28</i> upregulation. Photosynthetic activity was enhanced by <i>StCDPK28</i> overexpression, while decreased by <i>StCDPK2</i> knockdown under water deficiency and osmotic stress. <i>StCDPK28</i> overexpression decreased H<sub>2</sub>O<sub>2</sub> and MDA, and increased proline, while <i>StCDPK28</i> knockdown showed reverse results, compared with the wild type, in response to water deficiency and osmotic stress. <i>StCDPK28</i> overexpression increased the activities of CAT, SOD and POD, while <i>StCDPK28</i>-knockdown plants indicated the reverse trend under water deficiency and osmotic stress conditions. Regulation of StCDPK28 expression could be a promising approach to improve the tolerance ability of potato plants in response to drought or high salt media. |
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| ISSN: | 1661-6596 1422-0067 |