Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress
Calcium-dependent protein kinases (CDPK) are implicated in signaling transduction in eukaryotic organisms. It is largely unknown whether <i>StCDPK28</i> plays a role in the response to water deficiency and osmotic stress in potato plants (<i>Solanum tuberosum</i> L.). Potato...
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MDPI AG
2022-08-01
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| Series: | International Journal of Molecular Sciences |
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| author | Xi Zhu Fangfang Wang Shigui Li Ya Feng Jiangwei Yang Ning Zhang Huaijun Si |
| author_facet | Xi Zhu Fangfang Wang Shigui Li Ya Feng Jiangwei Yang Ning Zhang Huaijun Si |
| author_sort | Xi Zhu |
| collection | DOAJ |
| description | Calcium-dependent protein kinases (CDPK) are implicated in signaling transduction in eukaryotic organisms. It is largely unknown whether <i>StCDPK28</i> plays a role in the response to water deficiency and osmotic stress in potato plants (<i>Solanum tuberosum</i> L.). Potato cv. Zihuabai was cultivated under natural, moderate, and severe water deficiency conditions; to induce osmotic stress, potato plants were treated with 10% or 20% PEG. <i>StCDPK28</i>-overexpression and <i>StCDPK28</i>-knockdown plants were constructed. <i>StCDPKs</i> were evaluated by qRT-PCR. The subcellular location of the StCDPK28 protein was observed with confocal scanning laser microscopy. Phenotypic changes were indicated by photosynthetic activity, the contents of H<sub>2</sub>O<sub>2</sub>, MDA and proline, and the activities of CAT, SOD and POD. Results showed water deficiency and osmotic stress altered <i>StCDPK</i> expression patterns. StCDPK28 exhibited a membrane, cytosolic and nuclear localization. Water deficiency and osmotic stress induced <i>StCDPK28</i> upregulation. Photosynthetic activity was enhanced by <i>StCDPK28</i> overexpression, while decreased by <i>StCDPK2</i> knockdown under water deficiency and osmotic stress. <i>StCDPK28</i> overexpression decreased H<sub>2</sub>O<sub>2</sub> and MDA, and increased proline, while <i>StCDPK28</i> knockdown showed reverse results, compared with the wild type, in response to water deficiency and osmotic stress. <i>StCDPK28</i> overexpression increased the activities of CAT, SOD and POD, while <i>StCDPK28</i>-knockdown plants indicated the reverse trend under water deficiency and osmotic stress conditions. Regulation of StCDPK28 expression could be a promising approach to improve the tolerance ability of potato plants in response to drought or high salt media. |
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| spelling | doaj.art-adc7c652fa474dceb03304e8b3d54dc02023-12-01T22:58:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012315879510.3390/ijms23158795Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic StressXi Zhu0Fangfang Wang1Shigui Li2Ya Feng3Jiangwei Yang4Ning Zhang5Huaijun Si6State Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaState Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou 730070, ChinaCalcium-dependent protein kinases (CDPK) are implicated in signaling transduction in eukaryotic organisms. It is largely unknown whether <i>StCDPK28</i> plays a role in the response to water deficiency and osmotic stress in potato plants (<i>Solanum tuberosum</i> L.). Potato cv. Zihuabai was cultivated under natural, moderate, and severe water deficiency conditions; to induce osmotic stress, potato plants were treated with 10% or 20% PEG. <i>StCDPK28</i>-overexpression and <i>StCDPK28</i>-knockdown plants were constructed. <i>StCDPKs</i> were evaluated by qRT-PCR. The subcellular location of the StCDPK28 protein was observed with confocal scanning laser microscopy. Phenotypic changes were indicated by photosynthetic activity, the contents of H<sub>2</sub>O<sub>2</sub>, MDA and proline, and the activities of CAT, SOD and POD. Results showed water deficiency and osmotic stress altered <i>StCDPK</i> expression patterns. StCDPK28 exhibited a membrane, cytosolic and nuclear localization. Water deficiency and osmotic stress induced <i>StCDPK28</i> upregulation. Photosynthetic activity was enhanced by <i>StCDPK28</i> overexpression, while decreased by <i>StCDPK2</i> knockdown under water deficiency and osmotic stress. <i>StCDPK28</i> overexpression decreased H<sub>2</sub>O<sub>2</sub> and MDA, and increased proline, while <i>StCDPK28</i> knockdown showed reverse results, compared with the wild type, in response to water deficiency and osmotic stress. <i>StCDPK28</i> overexpression increased the activities of CAT, SOD and POD, while <i>StCDPK28</i>-knockdown plants indicated the reverse trend under water deficiency and osmotic stress conditions. Regulation of StCDPK28 expression could be a promising approach to improve the tolerance ability of potato plants in response to drought or high salt media.https://www.mdpi.com/1422-0067/23/15/8795<i>StCDPK28</i>drought stressosmotic stresspotato |
| spellingShingle | Xi Zhu Fangfang Wang Shigui Li Ya Feng Jiangwei Yang Ning Zhang Huaijun Si Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress International Journal of Molecular Sciences <i>StCDPK28</i> drought stress osmotic stress potato |
| title | Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress |
| title_full | Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress |
| title_fullStr | Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress |
| title_full_unstemmed | Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress |
| title_short | Calcium-Dependent Protein Kinase 28 Maintains Potato Photosynthesis and Its Tolerance under Water Deficiency and Osmotic Stress |
| title_sort | calcium dependent protein kinase 28 maintains potato photosynthesis and its tolerance under water deficiency and osmotic stress |
| topic | <i>StCDPK28</i> drought stress osmotic stress potato |
| url | https://www.mdpi.com/1422-0067/23/15/8795 |
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