Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shoots
Anthocyanins, a categories of metabolites derived from flavonoid biosynthesis pathway, are responsible for the red color of peach. Anthocyanin metabolism is modulated by a complicated regulatory network. In this study, our results demonstrated that PpNAC25 was a component of anthocyanin regulatory n...
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2022-01-01
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| Series: | Fruit Research |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0021 |
| _version_ | 1797293184518193152 |
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| author | Mingxi Geng Yun Shao Mengmeng Zhang Xianbo Zheng Bin Tan Wei Wang Langlang Zhang Xia Ye Ming Li Jidong Li Jun Cheng Jiancan Feng |
| author_facet | Mingxi Geng Yun Shao Mengmeng Zhang Xianbo Zheng Bin Tan Wei Wang Langlang Zhang Xia Ye Ming Li Jidong Li Jun Cheng Jiancan Feng |
| author_sort | Mingxi Geng |
| collection | DOAJ |
| description | Anthocyanins, a categories of metabolites derived from flavonoid biosynthesis pathway, are responsible for the red color of peach. Anthocyanin metabolism is modulated by a complicated regulatory network. In this study, our results demonstrated that PpNAC25 was a component of anthocyanin regulatory network in peach. PpNAC25 showed a closed relationship with the well-known ripening-related gene NOR (no-ripening) and was highly expressed in peach fruit. Overexpression of PpNAC25 in poplar resulted in a redder shoot tips compared with EV control. PpNAC25 overexpression upregulated the anthocyanin biosynthetic and transportation genes in transcript levels and then increased anthocyanin contents. In Y1H and Luc/Ren assay, PpNAC25 bound to the promoter of the anthocyanin-activator PpMYB10.1 and PpMYB10.2 and activated its transcript. Moreover, PpNAC25 formed a heterodimer with PpNAC1, an anthocyanin-regulator. Our researches provide evidence that PpNAC25 may be a positive regulator of anthocyanin biosynthesis in peach fruit. |
| first_indexed | 2024-03-07T20:09:12Z |
| format | Article |
| id | doaj.art-adc7dda9b7b043999cef02e0f2b1dd9f |
| institution | Directory Open Access Journal |
| issn | 2769-4615 |
| language | English |
| last_indexed | 2024-03-07T20:09:12Z |
| publishDate | 2022-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Fruit Research |
| spelling | doaj.art-adc7dda9b7b043999cef02e0f2b1dd9f2024-02-28T03:19:35ZengMaximum Academic PressFruit Research2769-46152022-01-01211910.48130/FruRes-2022-0021FruRes-2022-0021Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shootsMingxi Geng0Yun Shao1Mengmeng Zhang2Xianbo Zheng3Bin Tan4Wei Wang5Langlang Zhang6Xia Ye7Ming Li8Jidong Li9Jun Cheng10Jiancan Feng11College of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaCollege of Horticulture, Henan Agricultural University, 95 Wenhua Road, Zhengzhou 450002, ChinaAnthocyanins, a categories of metabolites derived from flavonoid biosynthesis pathway, are responsible for the red color of peach. Anthocyanin metabolism is modulated by a complicated regulatory network. In this study, our results demonstrated that PpNAC25 was a component of anthocyanin regulatory network in peach. PpNAC25 showed a closed relationship with the well-known ripening-related gene NOR (no-ripening) and was highly expressed in peach fruit. Overexpression of PpNAC25 in poplar resulted in a redder shoot tips compared with EV control. PpNAC25 overexpression upregulated the anthocyanin biosynthetic and transportation genes in transcript levels and then increased anthocyanin contents. In Y1H and Luc/Ren assay, PpNAC25 bound to the promoter of the anthocyanin-activator PpMYB10.1 and PpMYB10.2 and activated its transcript. Moreover, PpNAC25 formed a heterodimer with PpNAC1, an anthocyanin-regulator. Our researches provide evidence that PpNAC25 may be a positive regulator of anthocyanin biosynthesis in peach fruit.https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0021peachanthocyaninnac transcription factormyb 10.1. |
| spellingShingle | Mingxi Geng Yun Shao Mengmeng Zhang Xianbo Zheng Bin Tan Wei Wang Langlang Zhang Xia Ye Ming Li Jidong Li Jun Cheng Jiancan Feng Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shoots Fruit Research peach anthocyanin nac transcription factor myb 10.1. |
| title | Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shoots |
| title_full | Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shoots |
| title_fullStr | Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shoots |
| title_full_unstemmed | Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shoots |
| title_short | Overexpression of peach NAC25 promotes anthocyanin biosynthesis in poplar shoots |
| title_sort | overexpression of peach nac25 promotes anthocyanin biosynthesis in poplar shoots |
| topic | peach anthocyanin nac transcription factor myb 10.1. |
| url | https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0021 |
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