Antidiabetic phytodrug from Maerua angolensis DC: Formulation, standardization, in vitro and in vivo evaluations
Maerua angolensis is a shrub or small tree widely distributed in tropical Africa. The plant materials are used for various ethnomedicinal applications across the region, including the prevention and treatment of diabetes. This study was aimed to evaluate the antidiabetic potential of formulated free...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227623004805 |
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| author | Obi Peter Adigwe Bulus Adzu Florence David Tarfa Henry Omoregie Egharevba |
| author_facet | Obi Peter Adigwe Bulus Adzu Florence David Tarfa Henry Omoregie Egharevba |
| author_sort | Obi Peter Adigwe |
| collection | DOAJ |
| description | Maerua angolensis is a shrub or small tree widely distributed in tropical Africa. The plant materials are used for various ethnomedicinal applications across the region, including the prevention and treatment of diabetes. This study was aimed to evaluate the antidiabetic potential of formulated freeze-dried aqueous extract of Maerua angolensis leaves using standard in vitro and in vivo experimental models. Infusion extract of the plant's powdered dried leaves was prepared, the resultant extract was freeze-dried and formulated (denoted FIEMa). The physicochemical, quantitative and qualitative phytochemical constituents of FIEMa were determined using analytical techniques (UV-visible and HPLC). The antidiabetic activity of the reconstituted formulation was investigated using in vitro and in vivo models. The in vitro activity was assessed using Phosphomolybdenum assay and DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging activity for antioxidant effect, and α-amylase inhibition and α- glycosidase inhibition assays. The assays of the normoglycemic, oral glucose load tolerance, and single-dose alloxan (150 mg/kg) induced diabetes in rats were used for in vivo antidiabetic activity. The results show that FIEMa contains valuable phytochemicals. It exhibited antioxidant effect, α-amylase and α- glucosidase inhibition activities. FIEMa did not significantly reduce blood glucose levels (BGL) in normoglycemic mice but produced a significant reduction of BGL in the oral glucose tolerance test. In alloxan-induced diabetic rats, significant reductions of BGL were observed in groups treated with high doses of FIEMa in the single-dose study and from the second week onwards in the repeated dose study. It also exhibited less reduction in body weight compared to the control group. The findings indicate that FIEMa has an antidiabetic activity, low risk of hypoglycemia, and body weight loss. The valuable phytochemicals plausibly mediate this effect, especially the dominant betulinic acid detected in FIEMa, most likely acting in synergy. |
| first_indexed | 2024-03-07T15:46:44Z |
| format | Article |
| id | doaj.art-add81b7210a3415e92fbe5652b3c7d5b |
| institution | Directory Open Access Journal |
| issn | 2468-2276 |
| language | English |
| last_indexed | 2024-03-07T15:46:44Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj.art-add81b7210a3415e92fbe5652b3c7d5b2024-03-05T04:30:10ZengElsevierScientific African2468-22762024-03-0123e02026Antidiabetic phytodrug from Maerua angolensis DC: Formulation, standardization, in vitro and in vivo evaluationsObi Peter Adigwe0Bulus Adzu1Florence David Tarfa2Henry Omoregie Egharevba3Director General/Chief Executive Officer, National Institute for Pharmaceutical Research and Development (NIPRD), Plot 942, Cadastral Zone C16, Idu Industrial District 1B, P.M.B. 21, Garki, Abuja, NigeriaDepartment of Pharmacology and Toxicology, National Institute for Pharmaceutical Research and Development (NIPRD), Garki, Abuja, Nigeria; Corresponding author.Department of Medicinal Chemistry and Quality Control, National Institute for Pharmaceutical Research and Development (NIPRD), Garki, Abuja, NigeriaDepartment of Medicinal Plants Research and Traditional Medicine, National Institute for Pharmaceutical Research and Development (NIPRD), Garki, Abuja, NigeriaMaerua angolensis is a shrub or small tree widely distributed in tropical Africa. The plant materials are used for various ethnomedicinal applications across the region, including the prevention and treatment of diabetes. This study was aimed to evaluate the antidiabetic potential of formulated freeze-dried aqueous extract of Maerua angolensis leaves using standard in vitro and in vivo experimental models. Infusion extract of the plant's powdered dried leaves was prepared, the resultant extract was freeze-dried and formulated (denoted FIEMa). The physicochemical, quantitative and qualitative phytochemical constituents of FIEMa were determined using analytical techniques (UV-visible and HPLC). The antidiabetic activity of the reconstituted formulation was investigated using in vitro and in vivo models. The in vitro activity was assessed using Phosphomolybdenum assay and DPPH (2,2-Diphenyl-1-picrylhydrazyl) radical scavenging activity for antioxidant effect, and α-amylase inhibition and α- glycosidase inhibition assays. The assays of the normoglycemic, oral glucose load tolerance, and single-dose alloxan (150 mg/kg) induced diabetes in rats were used for in vivo antidiabetic activity. The results show that FIEMa contains valuable phytochemicals. It exhibited antioxidant effect, α-amylase and α- glucosidase inhibition activities. FIEMa did not significantly reduce blood glucose levels (BGL) in normoglycemic mice but produced a significant reduction of BGL in the oral glucose tolerance test. In alloxan-induced diabetic rats, significant reductions of BGL were observed in groups treated with high doses of FIEMa in the single-dose study and from the second week onwards in the repeated dose study. It also exhibited less reduction in body weight compared to the control group. The findings indicate that FIEMa has an antidiabetic activity, low risk of hypoglycemia, and body weight loss. The valuable phytochemicals plausibly mediate this effect, especially the dominant betulinic acid detected in FIEMa, most likely acting in synergy.http://www.sciencedirect.com/science/article/pii/S2468227623004805Maerua angolensisFormulationStandardisationAntidiabetic |
| spellingShingle | Obi Peter Adigwe Bulus Adzu Florence David Tarfa Henry Omoregie Egharevba Antidiabetic phytodrug from Maerua angolensis DC: Formulation, standardization, in vitro and in vivo evaluations Scientific African Maerua angolensis Formulation Standardisation Antidiabetic |
| title | Antidiabetic phytodrug from Maerua angolensis DC: Formulation, standardization, in vitro and in vivo evaluations |
| title_full | Antidiabetic phytodrug from Maerua angolensis DC: Formulation, standardization, in vitro and in vivo evaluations |
| title_fullStr | Antidiabetic phytodrug from Maerua angolensis DC: Formulation, standardization, in vitro and in vivo evaluations |
| title_full_unstemmed | Antidiabetic phytodrug from Maerua angolensis DC: Formulation, standardization, in vitro and in vivo evaluations |
| title_short | Antidiabetic phytodrug from Maerua angolensis DC: Formulation, standardization, in vitro and in vivo evaluations |
| title_sort | antidiabetic phytodrug from maerua angolensis dc formulation standardization in vitro and in vivo evaluations |
| topic | Maerua angolensis Formulation Standardisation Antidiabetic |
| url | http://www.sciencedirect.com/science/article/pii/S2468227623004805 |
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