Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases
India being a developing country mainly depends on livestock sector for its economy. However, nowadays, there is emergence and reemergence of more transboundary animal diseases. The existing diagnostic techniques are not so quick and with less specificity. To reduce the economy loss, there should be...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Veterinary World
2016-05-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.9/May-2016/6.pdf |
| _version_ | 1818645769869066240 |
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| author | Pravas Ranjan Sahoo Kamadev Sethy Swagat Mohapatra Debasis Panda |
| author_facet | Pravas Ranjan Sahoo Kamadev Sethy Swagat Mohapatra Debasis Panda |
| author_sort | Pravas Ranjan Sahoo |
| collection | DOAJ |
| description | India being a developing country mainly depends on livestock sector for its economy. However, nowadays, there is emergence and reemergence of more transboundary animal diseases. The existing diagnostic techniques are not so quick and with less specificity. To reduce the economy loss, there should be a development of rapid, reliable, robust diagnostic technique, which can work with high degree of sensitivity and specificity. Loop mediated isothermal amplification assay is a rapid gene amplification technique that amplifies nucleic acid under an isothermal condition with a set of designed primers spanning eight distinct sequences of the target. This assay can be used as an emerging powerful, innovative gene amplification diagnostic tool against various pathogens of livestock diseases. This review is to highlight the basic concept and methodology of this assay in livestock disease. |
| first_indexed | 2024-12-17T00:36:01Z |
| format | Article |
| id | doaj.art-add8ab4477ba47009bffe2a65443c40e |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-17T00:36:01Z |
| publishDate | 2016-05-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-add8ab4477ba47009bffe2a65443c40e2022-12-21T22:10:09ZengVeterinary WorldVeterinary World0972-89882231-09162016-05-019546546910.14202/vetworld.2016.465-469Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseasesPravas Ranjan Sahoo0Kamadev Sethy1Swagat Mohapatra2Debasis Panda3Department of Veterinary Biochemistry, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India; pravasvet86@gmail.comDepartment of Animal Nutrition, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India; babuivri@gmail.comDepartment of Veterinary Physiology, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India; swagat.physiology@gmail.comDepartment of Veterinary Physiology, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India; bapu8280@mail.comIndia being a developing country mainly depends on livestock sector for its economy. However, nowadays, there is emergence and reemergence of more transboundary animal diseases. The existing diagnostic techniques are not so quick and with less specificity. To reduce the economy loss, there should be a development of rapid, reliable, robust diagnostic technique, which can work with high degree of sensitivity and specificity. Loop mediated isothermal amplification assay is a rapid gene amplification technique that amplifies nucleic acid under an isothermal condition with a set of designed primers spanning eight distinct sequences of the target. This assay can be used as an emerging powerful, innovative gene amplification diagnostic tool against various pathogens of livestock diseases. This review is to highlight the basic concept and methodology of this assay in livestock disease.http://www.veterinaryworld.org/Vol.9/May-2016/6.pdfdesigned primersloop-mediated isothermal amplification assaytransboundary animal diseases |
| spellingShingle | Pravas Ranjan Sahoo Kamadev Sethy Swagat Mohapatra Debasis Panda Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases Veterinary World designed primers loop-mediated isothermal amplification assay transboundary animal diseases |
| title | Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases |
| title_full | Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases |
| title_fullStr | Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases |
| title_full_unstemmed | Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases |
| title_short | Loop mediated isothermal amplification: An innovative gene amplification technique for animal diseases |
| title_sort | loop mediated isothermal amplification an innovative gene amplification technique for animal diseases |
| topic | designed primers loop-mediated isothermal amplification assay transboundary animal diseases |
| url | http://www.veterinaryworld.org/Vol.9/May-2016/6.pdf |
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