Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of <i>Cornus macrophylla</i> Bioss (Bark)
The objective of the current study was to evaluate the phytochemical and pharmacological potential of the <i>Cornus macrophylla</i>. <i>C. macrophylla</i> belongs to the family Cornaceae. It is locally known as khadang and is used for the treatment of different diseases such...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/13/4081 |
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| author | Ali Khan Aini Pervaiz Bushra Ansari Riaz Ullah Syed Muhammad Mukarram Shah Haroon Khan Muhammad Saeed Jan Fida Hussain Mohammad Ijaz Khan Ghadeer M. Albadrani Ahmed E. Altyar Mohamed M. Abdel-Daim |
| author_facet | Ali Khan Aini Pervaiz Bushra Ansari Riaz Ullah Syed Muhammad Mukarram Shah Haroon Khan Muhammad Saeed Jan Fida Hussain Mohammad Ijaz Khan Ghadeer M. Albadrani Ahmed E. Altyar Mohamed M. Abdel-Daim |
| author_sort | Ali Khan |
| collection | DOAJ |
| description | The objective of the current study was to evaluate the phytochemical and pharmacological potential of the <i>Cornus macrophylla</i>. <i>C. macrophylla</i> belongs to the family Cornaceae. It is locally known as khadang and is used for the treatment of different diseases such as analgesic, tonic, diuretic, malaria, inflammation, allergy, infections, cancer, diabetes, and lipid peroxidative. The crude extract and different fractions of <i>C. macrophyll</i> were evaluated by gas chromatography and mass spectroscopy (GC-MS), which identified the most potent bioactive phytochemicals. The antioxidant ability of <i>C. macrophylla</i> was studied by 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and 1,1 diphenyl-2-picryl-hydrazyl (DPPH) methods. The crude and subsequent fractions of the <i>C. macrophylla</i> were also tested against anti-inflammatory enzymes using COX-2 (Cyclooxygenase-2) and 5-LOX (5-lipoxygenase) assays. The molecular docking was carried out using molecular operating environment (MOE) software. The GC-MS study of <i>C. macrophylla</i> confirmed forty-eight compounds in ethyl acetate (Et.AC) fraction and revealed that the Et.AC fraction was the most active fraction. The antioxidant ability of the Et.AC fraction showed an IC<sub>50</sub> values of 09.54 μg/mL and 7.8 μg/mL against ABTS and DPPH assay respectively. Among all the fractions of <i>C. macrophylla</i>, Et.AC showed excellent activity against COX-2 and 5-LOX enzyme. The observed IC<sub>50</sub> values were 93.35 μg/mL against COX-2 and 75.64 μg/mL for 5-LOX respectively. Molecular docking studies supported these in vitro results and confirmed the anti-inflammatory potential of <i>C. macrophylla. C. macrophylla</i> has promising potential as a source for the development of new drugs against inflammation in the future. |
| first_indexed | 2024-03-09T04:01:44Z |
| format | Article |
| id | doaj.art-adda69eb6e874415b81a14e6fc1dd397 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:01:44Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-adda69eb6e874415b81a14e6fc1dd3972023-12-03T14:13:16ZengMDPI AGMolecules1420-30492022-06-012713408110.3390/molecules27134081Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of <i>Cornus macrophylla</i> Bioss (Bark)Ali Khan0Aini Pervaiz1Bushra Ansari2Riaz Ullah3Syed Muhammad Mukarram Shah4Haroon Khan5Muhammad Saeed Jan6Fida Hussain7Mohammad Ijaz Khan8Ghadeer M. Albadrani9Ahmed E. Altyar10Mohamed M. Abdel-Daim11Department of Pharmacy, University of Swabi, Ambar 94640, PakistanDepartment of Pharmacy, University of Swabi, Ambar 94640, PakistanDepartement of Pharmacy, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmacy, University of Swabi, Ambar 94640, PakistanDepartement of Pharmacy, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Pharmacy, University of Swabi, Ambar 94640, PakistanDepartment of Pharmacy, University of Swabi, Ambar 94640, PakistanDepartment of Pharmacy, University of Swabi, Ambar 94640, PakistanDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi ArabiaDepartment Pharmacy Practice, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi ArabiaPharmacy Program, Department of Pharmaceutical Sciences, Batterjee Medical College, Jeddah 21442, Saudi ArabiaThe objective of the current study was to evaluate the phytochemical and pharmacological potential of the <i>Cornus macrophylla</i>. <i>C. macrophylla</i> belongs to the family Cornaceae. It is locally known as khadang and is used for the treatment of different diseases such as analgesic, tonic, diuretic, malaria, inflammation, allergy, infections, cancer, diabetes, and lipid peroxidative. The crude extract and different fractions of <i>C. macrophyll</i> were evaluated by gas chromatography and mass spectroscopy (GC-MS), which identified the most potent bioactive phytochemicals. The antioxidant ability of <i>C. macrophylla</i> was studied by 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid (ABTS) and 1,1 diphenyl-2-picryl-hydrazyl (DPPH) methods. The crude and subsequent fractions of the <i>C. macrophylla</i> were also tested against anti-inflammatory enzymes using COX-2 (Cyclooxygenase-2) and 5-LOX (5-lipoxygenase) assays. The molecular docking was carried out using molecular operating environment (MOE) software. The GC-MS study of <i>C. macrophylla</i> confirmed forty-eight compounds in ethyl acetate (Et.AC) fraction and revealed that the Et.AC fraction was the most active fraction. The antioxidant ability of the Et.AC fraction showed an IC<sub>50</sub> values of 09.54 μg/mL and 7.8 μg/mL against ABTS and DPPH assay respectively. Among all the fractions of <i>C. macrophylla</i>, Et.AC showed excellent activity against COX-2 and 5-LOX enzyme. The observed IC<sub>50</sub> values were 93.35 μg/mL against COX-2 and 75.64 μg/mL for 5-LOX respectively. Molecular docking studies supported these in vitro results and confirmed the anti-inflammatory potential of <i>C. macrophylla. C. macrophylla</i> has promising potential as a source for the development of new drugs against inflammation in the future.https://www.mdpi.com/1420-3049/27/13/4081GC-MSanti-inflammatoryantioxidant<i>Cornus macrophylla</i> |
| spellingShingle | Ali Khan Aini Pervaiz Bushra Ansari Riaz Ullah Syed Muhammad Mukarram Shah Haroon Khan Muhammad Saeed Jan Fida Hussain Mohammad Ijaz Khan Ghadeer M. Albadrani Ahmed E. Altyar Mohamed M. Abdel-Daim Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of <i>Cornus macrophylla</i> Bioss (Bark) Molecules GC-MS anti-inflammatory antioxidant <i>Cornus macrophylla</i> |
| title | Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of <i>Cornus macrophylla</i> Bioss (Bark) |
| title_full | Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of <i>Cornus macrophylla</i> Bioss (Bark) |
| title_fullStr | Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of <i>Cornus macrophylla</i> Bioss (Bark) |
| title_full_unstemmed | Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of <i>Cornus macrophylla</i> Bioss (Bark) |
| title_short | Phytochemical Profiling, Anti-Inflammatory, Anti-Oxidant and In-Silico Approach of <i>Cornus macrophylla</i> Bioss (Bark) |
| title_sort | phytochemical profiling anti inflammatory anti oxidant and in silico approach of i cornus macrophylla i bioss bark |
| topic | GC-MS anti-inflammatory antioxidant <i>Cornus macrophylla</i> |
| url | https://www.mdpi.com/1420-3049/27/13/4081 |
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