Evaluating the use of 3'-(<it>p</it>-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions
<p>Abstract</p> <p>Background</p> <p>Given the importance of highly reactive oxygen species (hROS) as reactants in a wide range of biological, photochemical, and environmental systems there is an interest in detection and quantification of these species. The extreme rea...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-08-01
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| Series: | Geochemical Transactions |
| Online Access: | http://www.geochemicaltransactions.com/content/10/1/8 |
| _version_ | 1818148471596646400 |
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| author | Hylton Shavonne N Pedigo Christopher E Cohn Corey A Simon Sanford R Schoonen Martin AA |
| author_facet | Hylton Shavonne N Pedigo Christopher E Cohn Corey A Simon Sanford R Schoonen Martin AA |
| author_sort | Hylton Shavonne N |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Given the importance of highly reactive oxygen species (hROS) as reactants in a wide range of biological, photochemical, and environmental systems there is an interest in detection and quantification of these species. The extreme reactivity of the hROS, which includes hydroxyl radicals, presents an analytical challenge. 3'-(<it>p</it>-Aminophenyl) fluorescein (APF) is a relatively new probe used for measuring hROS. Here, we further evaluate the use of APF as a method for the detection of hydroxyl radicals in particle suspensions.</p> <p>Results</p> <p>Particle-generated hROS can be quantified with an estimated detection limit of 50 nM. Measurements of hROS in two National Institute of Standards and Technology (NIST 2709 and 2710) soil suspensions and a pyrite suspension show non-linear particle dose-response curves for hROS generation. APF can also be used in solutions containing no dissolved molecular oxygen (O<sub>2</sub>) to determine the role of O<sub>2 </sub>in the formation of hROS. Results confirm that O<sub>2 </sub>is mechanistically important in the formation of hROS by dissolved ferrous iron and in pyrite suspensions.</p> <p>Conclusion</p> <p>Given the non-linear dose-response curves for particle generation of hROS, we recommend using several particle loadings in experiments aimed to compare particles for their hROS generation potential. The method presented here is specific to hROS and simple to perform. The analysis can be conducted in mobile labs as only basic laboratory equipment is required.</p> |
| first_indexed | 2024-12-11T12:51:40Z |
| format | Article |
| id | doaj.art-addb04c030ad4876bec47e58d6dda980 |
| institution | Directory Open Access Journal |
| issn | 1467-4866 |
| language | English |
| last_indexed | 2024-12-11T12:51:40Z |
| publishDate | 2009-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Geochemical Transactions |
| spelling | doaj.art-addb04c030ad4876bec47e58d6dda9802022-12-22T01:06:40ZengBMCGeochemical Transactions1467-48662009-08-01101810.1186/1467-4866-10-8Evaluating the use of 3'-(<it>p</it>-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensionsHylton Shavonne NPedigo Christopher ECohn Corey ASimon Sanford RSchoonen Martin AA<p>Abstract</p> <p>Background</p> <p>Given the importance of highly reactive oxygen species (hROS) as reactants in a wide range of biological, photochemical, and environmental systems there is an interest in detection and quantification of these species. The extreme reactivity of the hROS, which includes hydroxyl radicals, presents an analytical challenge. 3'-(<it>p</it>-Aminophenyl) fluorescein (APF) is a relatively new probe used for measuring hROS. Here, we further evaluate the use of APF as a method for the detection of hydroxyl radicals in particle suspensions.</p> <p>Results</p> <p>Particle-generated hROS can be quantified with an estimated detection limit of 50 nM. Measurements of hROS in two National Institute of Standards and Technology (NIST 2709 and 2710) soil suspensions and a pyrite suspension show non-linear particle dose-response curves for hROS generation. APF can also be used in solutions containing no dissolved molecular oxygen (O<sub>2</sub>) to determine the role of O<sub>2 </sub>in the formation of hROS. Results confirm that O<sub>2 </sub>is mechanistically important in the formation of hROS by dissolved ferrous iron and in pyrite suspensions.</p> <p>Conclusion</p> <p>Given the non-linear dose-response curves for particle generation of hROS, we recommend using several particle loadings in experiments aimed to compare particles for their hROS generation potential. The method presented here is specific to hROS and simple to perform. The analysis can be conducted in mobile labs as only basic laboratory equipment is required.</p>http://www.geochemicaltransactions.com/content/10/1/8 |
| spellingShingle | Hylton Shavonne N Pedigo Christopher E Cohn Corey A Simon Sanford R Schoonen Martin AA Evaluating the use of 3'-(<it>p</it>-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions Geochemical Transactions |
| title | Evaluating the use of 3'-(<it>p</it>-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions |
| title_full | Evaluating the use of 3'-(<it>p</it>-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions |
| title_fullStr | Evaluating the use of 3'-(<it>p</it>-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions |
| title_full_unstemmed | Evaluating the use of 3'-(<it>p</it>-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions |
| title_short | Evaluating the use of 3'-(<it>p</it>-Aminophenyl) fluorescein for determining the formation of highly reactive oxygen species in particle suspensions |
| title_sort | evaluating the use of 3 it p it aminophenyl fluorescein for determining the formation of highly reactive oxygen species in particle suspensions |
| url | http://www.geochemicaltransactions.com/content/10/1/8 |
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