Highly Sensitive Fluorescence Sensor for Carrageenan from a Composite Methylcellulose/Polyacrylate Membrane
Carrageenans are linear sulphated polysaccharides that are commonly added into confectionery products but may exert a detrimental effect to human health. A new and simpler way of carrageenan determination based on an optical sensor utilizing a methylcellulose/poly(n-butyl acrylate) (Mc/PnBA) composi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-09-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/20/18/5043 |
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| author | Riyadh Abdulmalek Hassan Lee Yook Heng Ling Ling Tan |
| author_facet | Riyadh Abdulmalek Hassan Lee Yook Heng Ling Ling Tan |
| author_sort | Riyadh Abdulmalek Hassan |
| collection | DOAJ |
| description | Carrageenans are linear sulphated polysaccharides that are commonly added into confectionery products but may exert a detrimental effect to human health. A new and simpler way of carrageenan determination based on an optical sensor utilizing a methylcellulose/poly(n-butyl acrylate) (Mc/PnBA) composite membrane with immobilized methylene blue (MB) was developed. The hydrophilic Mc polymer membrane was successfully modified with a more hydrophobic acrylic polymer. This was to produce an insoluble membrane at room temperature where MB reagent could be immobilized to build an optical sensor for carrageenan analysis. The fluorescence intensity of MB in the composite membrane was found to be proportional to the carrageenan concentrations in a linear manner (1.0–20.0 mg L<sup>−1</sup>, R<sup>2</sup> = 0.992) and with a detection limit at 0.4 mg L<sup>−1</sup>. Recovery of spiked carrageenan into commercial fruit juice products showed percentage recoveries between 90% and 102%. The optical sensor has the advantages of improved sensitivity and better selectivity to carrageenan when compared to other types of hydrocolloids. Its sensitivity was comparable to most sophisticated techniques for carageenan analysis but better than other types of optical sensors. Thus, this sensor provides a simple, rapid, and sensitive means for carageenan analysis. |
| first_indexed | 2024-03-10T16:33:08Z |
| format | Article |
| id | doaj.art-addb9cae3acb48228bc2bc9e59d582db |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T16:33:08Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-addb9cae3acb48228bc2bc9e59d582db2023-11-20T12:40:16ZengMDPI AGSensors1424-82202020-09-012018504310.3390/s20185043Highly Sensitive Fluorescence Sensor for Carrageenan from a Composite Methylcellulose/Polyacrylate MembraneRiyadh Abdulmalek Hassan0Lee Yook Heng1Ling Ling Tan2School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, MalaysiaSchool of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, MalaysiaSoutheast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, MalaysiaCarrageenans are linear sulphated polysaccharides that are commonly added into confectionery products but may exert a detrimental effect to human health. A new and simpler way of carrageenan determination based on an optical sensor utilizing a methylcellulose/poly(n-butyl acrylate) (Mc/PnBA) composite membrane with immobilized methylene blue (MB) was developed. The hydrophilic Mc polymer membrane was successfully modified with a more hydrophobic acrylic polymer. This was to produce an insoluble membrane at room temperature where MB reagent could be immobilized to build an optical sensor for carrageenan analysis. The fluorescence intensity of MB in the composite membrane was found to be proportional to the carrageenan concentrations in a linear manner (1.0–20.0 mg L<sup>−1</sup>, R<sup>2</sup> = 0.992) and with a detection limit at 0.4 mg L<sup>−1</sup>. Recovery of spiked carrageenan into commercial fruit juice products showed percentage recoveries between 90% and 102%. The optical sensor has the advantages of improved sensitivity and better selectivity to carrageenan when compared to other types of hydrocolloids. Its sensitivity was comparable to most sophisticated techniques for carageenan analysis but better than other types of optical sensors. Thus, this sensor provides a simple, rapid, and sensitive means for carageenan analysis.https://www.mdpi.com/1424-8220/20/18/5043fluorescence sensorpolymer blendcarrageenanmethylene blue |
| spellingShingle | Riyadh Abdulmalek Hassan Lee Yook Heng Ling Ling Tan Highly Sensitive Fluorescence Sensor for Carrageenan from a Composite Methylcellulose/Polyacrylate Membrane Sensors fluorescence sensor polymer blend carrageenan methylene blue |
| title | Highly Sensitive Fluorescence Sensor for Carrageenan from a Composite Methylcellulose/Polyacrylate Membrane |
| title_full | Highly Sensitive Fluorescence Sensor for Carrageenan from a Composite Methylcellulose/Polyacrylate Membrane |
| title_fullStr | Highly Sensitive Fluorescence Sensor for Carrageenan from a Composite Methylcellulose/Polyacrylate Membrane |
| title_full_unstemmed | Highly Sensitive Fluorescence Sensor for Carrageenan from a Composite Methylcellulose/Polyacrylate Membrane |
| title_short | Highly Sensitive Fluorescence Sensor for Carrageenan from a Composite Methylcellulose/Polyacrylate Membrane |
| title_sort | highly sensitive fluorescence sensor for carrageenan from a composite methylcellulose polyacrylate membrane |
| topic | fluorescence sensor polymer blend carrageenan methylene blue |
| url | https://www.mdpi.com/1424-8220/20/18/5043 |
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