Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water
Abstract Background A visual, rapid, simple method was developed based on a loop-mediated isothermal amplification (LAMP) assay to detect Vibrio vulnificus in aquatic products and aquaculture waters. Results Genomic DNA was extracted from Vibrio vulnificus using the boiling method, and optimized pri...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-10-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12866-022-02656-1 |
| _version_ | 1797986036083589120 |
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| author | Zhuo Tian Lili Yang Xin Qi Qiuyue Zheng Dejing Shang Jijuan Cao |
| author_facet | Zhuo Tian Lili Yang Xin Qi Qiuyue Zheng Dejing Shang Jijuan Cao |
| author_sort | Zhuo Tian |
| collection | DOAJ |
| description | Abstract Background A visual, rapid, simple method was developed based on a loop-mediated isothermal amplification (LAMP) assay to detect Vibrio vulnificus in aquatic products and aquaculture waters. Results Genomic DNA was extracted from Vibrio vulnificus using the boiling method, and optimized primers were used to detect the gyrB gene using a visual LAMP method. The sensitivity of the assay was 10 fg/μL, and the obtained results were stable and reliable. Out of 655 aquatic product samples and 558 aquaculture water samples, the positive rates of Vibrio vulnificus detection were 9.01% and 8.60%, respectively, which are markedly higher than those of the traditional culture identification methods. Conclusion The relatively simple technical requirements, low equipment cost, and rapid detection make the visual LAMP method for the detection of Vibrio vulnificus a convenient choice for field detection in the aquaculture industry. |
| first_indexed | 2024-04-11T07:26:45Z |
| format | Article |
| id | doaj.art-adde0576473e450088bafca99fe6b5d7 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-11T07:26:45Z |
| publishDate | 2022-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-adde0576473e450088bafca99fe6b5d72022-12-22T04:37:02ZengBMCBMC Microbiology1471-21802022-10-0122111110.1186/s12866-022-02656-1Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental waterZhuo Tian0Lili Yang1Xin Qi2Qiuyue Zheng3Dejing Shang4Jijuan Cao5Liaoning Normal UniversityKey Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu UniversityDalian Customs Technology CenterKey Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu UniversityLiaoning Normal UniversityKey Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu UniversityAbstract Background A visual, rapid, simple method was developed based on a loop-mediated isothermal amplification (LAMP) assay to detect Vibrio vulnificus in aquatic products and aquaculture waters. Results Genomic DNA was extracted from Vibrio vulnificus using the boiling method, and optimized primers were used to detect the gyrB gene using a visual LAMP method. The sensitivity of the assay was 10 fg/μL, and the obtained results were stable and reliable. Out of 655 aquatic product samples and 558 aquaculture water samples, the positive rates of Vibrio vulnificus detection were 9.01% and 8.60%, respectively, which are markedly higher than those of the traditional culture identification methods. Conclusion The relatively simple technical requirements, low equipment cost, and rapid detection make the visual LAMP method for the detection of Vibrio vulnificus a convenient choice for field detection in the aquaculture industry.https://doi.org/10.1186/s12866-022-02656-1Vibrio vulnificusAquatic productsAquaculture watersVisualLoop-mediated isothermal amplification (LAMP) |
| spellingShingle | Zhuo Tian Lili Yang Xin Qi Qiuyue Zheng Dejing Shang Jijuan Cao Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water BMC Microbiology Vibrio vulnificus Aquatic products Aquaculture waters Visual Loop-mediated isothermal amplification (LAMP) |
| title | Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water |
| title_full | Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water |
| title_fullStr | Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water |
| title_full_unstemmed | Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water |
| title_short | Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water |
| title_sort | visual lamp method for the detection of vibrio vulnificus in aquatic products and environmental water |
| topic | Vibrio vulnificus Aquatic products Aquaculture waters Visual Loop-mediated isothermal amplification (LAMP) |
| url | https://doi.org/10.1186/s12866-022-02656-1 |
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