Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective Fraction
Background & Aims: Today, the emergence of antimicrobial resistance against conventional antibiotics is increasing. Many researchers consider plants with antimicrobial properties as a good alternative. In this study, the antimicrobial activity of Ajuga chamaecistus Ging. Subsp. Scoparia (Bioss)...
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| Format: | Article |
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Kerman University of Medical Sciences
2014-07-01
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| Series: | Journal of Kerman University of Medical Sciences |
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| Online Access: | https://jkmu.kmu.ac.ir/article_16201_51e6853c32a1347378c56d268bba7119.pdf |
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| author | M.H Moshefi M Mehrabani M Moeini F Saffari |
| author_facet | M.H Moshefi M Mehrabani M Moeini F Saffari |
| author_sort | M.H Moshefi |
| collection | DOAJ |
| description | Background & Aims: Today, the emergence of antimicrobial resistance against conventional antibiotics is increasing. Many researchers consider plants with antimicrobial properties as a good alternative. In this study, the antimicrobial activity of Ajuga chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. on 8 bacterial strains wasinvestigated. Methods: The leaf extract was prepared by methanolic maceration. The concentrated, dried extract was fractionated by different solvents including petroleum ether, dichloro methane, and ethyl acetate. Determination of minimum inhibitory concentration (MIC) of crude extract was performed using agar dilution method. Disk diffusion method was used for antimicrobial assay of different fractions. Then, the chemical constituents of the most effective fraction were separated on thin-layer chromatography (TLC) plates. Then, the prepared chromatograms were analyzed using immersion bioautography. Results: According to disk diffusion method, ethyl acetate fraction had stronger antibacterial activity against all tested bacteria. Bioautography of thisfraction showed antibacterial activity against Staphylococcus aureus in Rf = 0.9. However, all tested bacteria had inhibition spots in the site of fraction loading. Conclusion: Antibacterial activity of ethyl acetate fraction will appear if all components are used together. |
| first_indexed | 2024-03-13T02:08:23Z |
| format | Article |
| id | doaj.art-addf322b83084feeb9120c922a4f637c |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:08:23Z |
| publishDate | 2014-07-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-addf322b83084feeb9120c922a4f637c2023-07-01T05:25:39ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432014-07-0121431332016201Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective FractionM.H Moshefi0M Mehrabani1M Moeini2F Saffari3Professor, Department of Pharmaceutics, School of Pharmacy, Kerman University of Medial Sciences, Kerman, IranAssociate Professor, Department of Pharmacognosy, School of Pharmacy, Kerman University of Medical Sciences, Kerman, IranPharmacistPh.D. Candidate of Medical Bacteriology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, IranBackground & Aims: Today, the emergence of antimicrobial resistance against conventional antibiotics is increasing. Many researchers consider plants with antimicrobial properties as a good alternative. In this study, the antimicrobial activity of Ajuga chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. on 8 bacterial strains wasinvestigated. Methods: The leaf extract was prepared by methanolic maceration. The concentrated, dried extract was fractionated by different solvents including petroleum ether, dichloro methane, and ethyl acetate. Determination of minimum inhibitory concentration (MIC) of crude extract was performed using agar dilution method. Disk diffusion method was used for antimicrobial assay of different fractions. Then, the chemical constituents of the most effective fraction were separated on thin-layer chromatography (TLC) plates. Then, the prepared chromatograms were analyzed using immersion bioautography. Results: According to disk diffusion method, ethyl acetate fraction had stronger antibacterial activity against all tested bacteria. Bioautography of thisfraction showed antibacterial activity against Staphylococcus aureus in Rf = 0.9. However, all tested bacteria had inhibition spots in the site of fraction loading. Conclusion: Antibacterial activity of ethyl acetate fraction will appear if all components are used together.https://jkmu.kmu.ac.ir/article_16201_51e6853c32a1347378c56d268bba7119.pdfajuga chamaecistusantibacterial activityimmersion bioautography |
| spellingShingle | M.H Moshefi M Mehrabani M Moeini F Saffari Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective Fraction Journal of Kerman University of Medical Sciences ajuga chamaecistus antibacterial activity immersion bioautography |
| title | Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective Fraction |
| title_full | Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective Fraction |
| title_fullStr | Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective Fraction |
| title_full_unstemmed | Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective Fraction |
| title_short | Study of Antibacterial Effects of Different Fractions of Leaves Extract of Ajuga Chamaecistus Ging. Subsp. Scoparia (Bioss) Rech. f. and Bioautography of Effective Fraction |
| title_sort | study of antibacterial effects of different fractions of leaves extract of ajuga chamaecistus ging subsp scoparia bioss rech f and bioautography of effective fraction |
| topic | ajuga chamaecistus antibacterial activity immersion bioautography |
| url | https://jkmu.kmu.ac.ir/article_16201_51e6853c32a1347378c56d268bba7119.pdf |
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