Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s.
Insecticides remain a main tool for the control of arthropod vectors. The urgency to prevent the insurgence of insecticide resistance and the perspective to find new target sites, for the development of novel molecules, are fuelling the study of the molecular mechanisms involved in insect defence ag...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-03-01
|
| Series: | Insects |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2075-4450/10/3/66 |
| _version_ | 1818837757928144896 |
|---|---|
| author | Valentina Mastrantonio Marco Ferrari Agata Negri Tommaso Sturmo Guido Favia Daniele Porretta Sara Epis Sandra Urbanelli |
| author_facet | Valentina Mastrantonio Marco Ferrari Agata Negri Tommaso Sturmo Guido Favia Daniele Porretta Sara Epis Sandra Urbanelli |
| author_sort | Valentina Mastrantonio |
| collection | DOAJ |
| description | Insecticides remain a main tool for the control of arthropod vectors. The urgency to prevent the insurgence of insecticide resistance and the perspective to find new target sites, for the development of novel molecules, are fuelling the study of the molecular mechanisms involved in insect defence against xenobiotic compounds. In this study, we have investigated if ATP-binding cassette (ABC) transporters, a major component of the defensome machinery, are involved in defence against the insecticide permethrin, in susceptible larvae of the malaria vector Anopheles gambiae sensu stricto. Bioassays were performed with permethrin alone, or in combination with an ABC transporter inhibitor. Then we have investigated the expression profiles of five ABC transporter genes at different time points following permethrin exposure, to assess their expression patterns across time. The inhibition of ABC transporters increased the larval mortality by about 15-fold. Likewise, three genes were up-regulated after exposure to permethrin, showing different patterns of expression across the 48 h. Our results provide the first evidences of ABC transporters involvement in defence against a toxic in larvae of An. gambiae s.s. and show that the gene expression response is modulated across time, being continuous, but stronger at the earliest and latest times after exposure. |
| first_indexed | 2024-12-19T03:27:35Z |
| format | Article |
| id | doaj.art-addf38225e0040599dc3f3e128fbc478 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-12-19T03:27:35Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-addf38225e0040599dc3f3e128fbc4782022-12-21T20:37:34ZengMDPI AGInsects2075-44502019-03-011036610.3390/insects10030066insects10030066Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s.Valentina Mastrantonio0Marco Ferrari1Agata Negri2Tommaso Sturmo3Guido Favia4Daniele Porretta5Sara Epis6Sandra Urbanelli7Department of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biosciences and Pediatric Clinical Research Center, University of Milan, 20133 Milan, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalySchool of Bioscience and Veterinary Medicine, University of Camerino, 62032 Macerata, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biosciences and Pediatric Clinical Research Center, University of Milan, 20133 Milan, ItalyDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, ItalyInsecticides remain a main tool for the control of arthropod vectors. The urgency to prevent the insurgence of insecticide resistance and the perspective to find new target sites, for the development of novel molecules, are fuelling the study of the molecular mechanisms involved in insect defence against xenobiotic compounds. In this study, we have investigated if ATP-binding cassette (ABC) transporters, a major component of the defensome machinery, are involved in defence against the insecticide permethrin, in susceptible larvae of the malaria vector Anopheles gambiae sensu stricto. Bioassays were performed with permethrin alone, or in combination with an ABC transporter inhibitor. Then we have investigated the expression profiles of five ABC transporter genes at different time points following permethrin exposure, to assess their expression patterns across time. The inhibition of ABC transporters increased the larval mortality by about 15-fold. Likewise, three genes were up-regulated after exposure to permethrin, showing different patterns of expression across the 48 h. Our results provide the first evidences of ABC transporters involvement in defence against a toxic in larvae of An. gambiae s.s. and show that the gene expression response is modulated across time, being continuous, but stronger at the earliest and latest times after exposure.http://www.mdpi.com/2075-4450/10/3/66insecticide stressmosquitoesvector-controlchemical defensomeABC transporterspyrethroids |
| spellingShingle | Valentina Mastrantonio Marco Ferrari Agata Negri Tommaso Sturmo Guido Favia Daniele Porretta Sara Epis Sandra Urbanelli Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s. Insects insecticide stress mosquitoes vector-control chemical defensome ABC transporters pyrethroids |
| title | Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s. |
| title_full | Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s. |
| title_fullStr | Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s. |
| title_full_unstemmed | Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s. |
| title_short | Insecticide Exposure Triggers a Modulated Expression of ABC Transporter Genes in Larvae of Anopheles gambiae s.s. |
| title_sort | insecticide exposure triggers a modulated expression of abc transporter genes in larvae of anopheles gambiae s s |
| topic | insecticide stress mosquitoes vector-control chemical defensome ABC transporters pyrethroids |
| url | http://www.mdpi.com/2075-4450/10/3/66 |
| work_keys_str_mv | AT valentinamastrantonio insecticideexposuretriggersamodulatedexpressionofabctransportergenesinlarvaeofanophelesgambiaess AT marcoferrari insecticideexposuretriggersamodulatedexpressionofabctransportergenesinlarvaeofanophelesgambiaess AT agatanegri insecticideexposuretriggersamodulatedexpressionofabctransportergenesinlarvaeofanophelesgambiaess AT tommasosturmo insecticideexposuretriggersamodulatedexpressionofabctransportergenesinlarvaeofanophelesgambiaess AT guidofavia insecticideexposuretriggersamodulatedexpressionofabctransportergenesinlarvaeofanophelesgambiaess AT danieleporretta insecticideexposuretriggersamodulatedexpressionofabctransportergenesinlarvaeofanophelesgambiaess AT saraepis insecticideexposuretriggersamodulatedexpressionofabctransportergenesinlarvaeofanophelesgambiaess AT sandraurbanelli insecticideexposuretriggersamodulatedexpressionofabctransportergenesinlarvaeofanophelesgambiaess |