Epidemiological Evidence for Fecal-Oral Transmission of Murine Kobuvirus
BackgroundMurine Kobuvirus (MuKV) is a novel picornavirus of the genus Kobuvirus, and was first identified in the feces of murine rodents in the USA in 2011. There is limited information on the transmission route of MuKV. Thus, we conducted a study to investigate virus detection rates in fecal, seru...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2022.865605/full |
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| author | Yuhan Gao Wenqiao He Jiaqi Fu Yongzhi Li Huan He Qing Chen |
| author_facet | Yuhan Gao Wenqiao He Jiaqi Fu Yongzhi Li Huan He Qing Chen |
| author_sort | Yuhan Gao |
| collection | DOAJ |
| description | BackgroundMurine Kobuvirus (MuKV) is a novel picornavirus of the genus Kobuvirus, and was first identified in the feces of murine rodents in the USA in 2011. There is limited information on the transmission route of MuKV. Thus, we conducted a study to investigate virus detection rates in fecal, serum, throat, and lung tissue samples from murine rodents.ResultsA total of 413 fecal samples, 385 lung samples, 269 throat swab samples, and 183 serum samples were collected from 413 murine rodents (Rattus norvegicus, Rattus tanezumi, and Rattus rattus) captured in urban Shenzhen. Kobuviruses were detected via RT-PCR. Only fecal samples were positive, with prevalence rates of 34.9% in Rattus norvegicus and 29.4% in Rattus tanezumi. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions indicated that all of the MuKV sequences obtained belonged to Aichivirus A, and were genetically closely related to other MuKVs reported in China, Hungary, and the USA. Twenty-eight full-length MuKV sequences were acquired. Phylogenetic analysis of two sequences randomly selected from the two species (SZ59 and SZ171) indicated that they shared very high nucleotide and amino acid identity with one another (94.0 and 99.3%, respectively), and comparison with human Kobuvirus revealed amino acid identity values of ~80%. Additionally, a sewage-derived sequence shared high similarity with the rat-derived sequences identified in this study, with respective nucleotide and amino acid identity values from 86.5 and 90.7% to 87.2 and 91.1%.ConclusionThe results of the current study provide evidence that murine Kobuvirus is transmitted via the fecal-oral route. |
| first_indexed | 2024-04-13T06:48:38Z |
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| language | English |
| last_indexed | 2024-04-13T06:48:38Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-addfa6500390477988352502efac82552022-12-22T02:57:28ZengFrontiers Media S.A.Frontiers in Public Health2296-25652022-04-011010.3389/fpubh.2022.865605865605Epidemiological Evidence for Fecal-Oral Transmission of Murine KobuvirusYuhan GaoWenqiao HeJiaqi FuYongzhi LiHuan HeQing ChenBackgroundMurine Kobuvirus (MuKV) is a novel picornavirus of the genus Kobuvirus, and was first identified in the feces of murine rodents in the USA in 2011. There is limited information on the transmission route of MuKV. Thus, we conducted a study to investigate virus detection rates in fecal, serum, throat, and lung tissue samples from murine rodents.ResultsA total of 413 fecal samples, 385 lung samples, 269 throat swab samples, and 183 serum samples were collected from 413 murine rodents (Rattus norvegicus, Rattus tanezumi, and Rattus rattus) captured in urban Shenzhen. Kobuviruses were detected via RT-PCR. Only fecal samples were positive, with prevalence rates of 34.9% in Rattus norvegicus and 29.4% in Rattus tanezumi. Phylogenetic analysis based on partial 3D and complete VP1 sequence regions indicated that all of the MuKV sequences obtained belonged to Aichivirus A, and were genetically closely related to other MuKVs reported in China, Hungary, and the USA. Twenty-eight full-length MuKV sequences were acquired. Phylogenetic analysis of two sequences randomly selected from the two species (SZ59 and SZ171) indicated that they shared very high nucleotide and amino acid identity with one another (94.0 and 99.3%, respectively), and comparison with human Kobuvirus revealed amino acid identity values of ~80%. Additionally, a sewage-derived sequence shared high similarity with the rat-derived sequences identified in this study, with respective nucleotide and amino acid identity values from 86.5 and 90.7% to 87.2 and 91.1%.ConclusionThe results of the current study provide evidence that murine Kobuvirus is transmitted via the fecal-oral route.https://www.frontiersin.org/articles/10.3389/fpubh.2022.865605/fullgenetic characteristicKobuvirusmurine rodentphylogenetic analysisprevalencetransmission route |
| spellingShingle | Yuhan Gao Wenqiao He Jiaqi Fu Yongzhi Li Huan He Qing Chen Epidemiological Evidence for Fecal-Oral Transmission of Murine Kobuvirus Frontiers in Public Health genetic characteristic Kobuvirus murine rodent phylogenetic analysis prevalence transmission route |
| title | Epidemiological Evidence for Fecal-Oral Transmission of Murine Kobuvirus |
| title_full | Epidemiological Evidence for Fecal-Oral Transmission of Murine Kobuvirus |
| title_fullStr | Epidemiological Evidence for Fecal-Oral Transmission of Murine Kobuvirus |
| title_full_unstemmed | Epidemiological Evidence for Fecal-Oral Transmission of Murine Kobuvirus |
| title_short | Epidemiological Evidence for Fecal-Oral Transmission of Murine Kobuvirus |
| title_sort | epidemiological evidence for fecal oral transmission of murine kobuvirus |
| topic | genetic characteristic Kobuvirus murine rodent phylogenetic analysis prevalence transmission route |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2022.865605/full |
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