Chemopreventive Efficacy of Ginger Extract (Zingiber officinale)
The liver cells were cultured in the presence of ginger extract at various concentrations (0-1 mg /ml) for 24 h and the cells viability and proliferation rate were evaluated by MTS and BrdU assays, while apoptosis was evaluated by colorimetric determination of caspase 8 and 3 activities. Ginger extr...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2010-06-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/19 |
| _version_ | 1811231743102943232 |
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| author | Harliansyah Harliansyah W.N. Wan Zurinah M.Y. Yasmin Anum M. Noor Azian |
| author_facet | Harliansyah Harliansyah W.N. Wan Zurinah M.Y. Yasmin Anum M. Noor Azian |
| author_sort | Harliansyah Harliansyah |
| collection | DOAJ |
| description | The liver cells were cultured in the presence of ginger extract at various concentrations (0-1 mg /ml) for 24 h and the cells viability and proliferation rate were evaluated by MTS and BrdU assays, while apoptosis was evaluated by colorimetric determination of caspase 8 and 3 activities. Ginger extract exhibited a dose dependent inhibition of viability and proliferation of WRL-68, HLE and HepG2 cells with IC50 of 569.69 ± 7.99 µg/ml, 389.71 ± 26.56 µg/ml and 358.71 ± 17.12 µg/ml respectively. Ginger extract induced apoptosis through activation of caspase-8 and 3 in a dose dependent pattern for all cells at concentration ranging from 0-500 mg/ml. We found that antiproliferative effect of ginger extract could be associated with induction of apoptosis as shown by increased activities of caspase 8 and 3.The results from this study suggest that ginger extract has chemopreventive properties against hepatoma cells HepG2 and HLE by inhibiting cellular proliferation and inducing apoptosis.
Keywords : antiproliferation, apoptosis, caspases, Zingiber officinale |
| first_indexed | 2024-04-12T10:50:12Z |
| format | Article |
| id | doaj.art-ade13d2f5abf432482a9294c7486a24c |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-04-12T10:50:12Z |
| publishDate | 2010-06-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-ade13d2f5abf432482a9294c7486a24c2022-12-22T03:36:16ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892010-06-011211812310.14499/indonesianjcanchemoprev1iss2pp118-12320Chemopreventive Efficacy of Ginger Extract (Zingiber officinale)Harliansyah Harliansyah0W.N. Wan Zurinah1M.Y. Yasmin Anum2M. Noor Azian3Departement of Biochemistry, Yarsi University School of Medicine, JakartaDepartement of Biochemistry, Faculty of Medicine, University Kebangsaan MalaysiaDepartement of Biochemistry, Faculty of Medicine, University Kebangsaan MalaysiaCentre of Lipids and Engineering and Applied Research, Universiti Teknologi MalaysiaThe liver cells were cultured in the presence of ginger extract at various concentrations (0-1 mg /ml) for 24 h and the cells viability and proliferation rate were evaluated by MTS and BrdU assays, while apoptosis was evaluated by colorimetric determination of caspase 8 and 3 activities. Ginger extract exhibited a dose dependent inhibition of viability and proliferation of WRL-68, HLE and HepG2 cells with IC50 of 569.69 ± 7.99 µg/ml, 389.71 ± 26.56 µg/ml and 358.71 ± 17.12 µg/ml respectively. Ginger extract induced apoptosis through activation of caspase-8 and 3 in a dose dependent pattern for all cells at concentration ranging from 0-500 mg/ml. We found that antiproliferative effect of ginger extract could be associated with induction of apoptosis as shown by increased activities of caspase 8 and 3.The results from this study suggest that ginger extract has chemopreventive properties against hepatoma cells HepG2 and HLE by inhibiting cellular proliferation and inducing apoptosis. Keywords : antiproliferation, apoptosis, caspases, Zingiber officinalehttps://ijcc.chemoprev.org/index.php/ijcc/article/view/19 |
| spellingShingle | Harliansyah Harliansyah W.N. Wan Zurinah M.Y. Yasmin Anum M. Noor Azian Chemopreventive Efficacy of Ginger Extract (Zingiber officinale) ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Chemopreventive Efficacy of Ginger Extract (Zingiber officinale) |
| title_full | Chemopreventive Efficacy of Ginger Extract (Zingiber officinale) |
| title_fullStr | Chemopreventive Efficacy of Ginger Extract (Zingiber officinale) |
| title_full_unstemmed | Chemopreventive Efficacy of Ginger Extract (Zingiber officinale) |
| title_short | Chemopreventive Efficacy of Ginger Extract (Zingiber officinale) |
| title_sort | chemopreventive efficacy of ginger extract zingiber officinale |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/19 |
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