Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathway
Abstract Background Gastric cancer (GC) ranks fourth among the causes of death from malignant tumors in the world. Studies have implicated the dysregulation of circRNAs with GC. However, the relationship between hsa‐circ‐0052001 and GC is unclear. Methods In our current study, we assessed the expres...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-03-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.5446 |
| _version_ | 1797854220547784704 |
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| author | Qixuan Xu Yizhou Yao Haishun Ni Jinrong Gu Xuchao Wang Linhua Jiang Bin Wang Xinguo Zhu |
| author_facet | Qixuan Xu Yizhou Yao Haishun Ni Jinrong Gu Xuchao Wang Linhua Jiang Bin Wang Xinguo Zhu |
| author_sort | Qixuan Xu |
| collection | DOAJ |
| description | Abstract Background Gastric cancer (GC) ranks fourth among the causes of death from malignant tumors in the world. Studies have implicated the dysregulation of circRNAs with GC. However, the relationship between hsa‐circ‐0052001 and GC is unclear. Methods In our current study, we assessed the expression levels of hsa‐circ‐0052001 in GC cells and tissues using quantitative real‐time PCR (qPCR). The role of hsa‐circ‐0052001 expression on the proliferation and invasion of GC cells was assessed using in vitro experiments. The role of hsa‐circ‐0052001 on the proliferation of GC cells was also analyzed using in vivo models. The pathways downstream of hsa‐circ‐0052001 were identified using bioinformatics analyses, western blot (WB) assays, and qRT‐PCR. Results We found that compared with normal gastric mucosa epithelial cells and adjacent paracancer tissues, hsa‐circ‐0052001 was overexpressed in GC cells and tissues. Also, the hsa‐circ‐0052001 level was linked to patient clinicopathological characteristics of GC. Cell proliferation and metastatic ability were inhibited in gastric cancer cells when hsa‐circ‐0052001 was knocked down in vitro and cancer growth in vivo. Mechanistically, hsa‐circ‐0052001 promoted the carcinogenesis of GC cells via the MAPK signal pathway. Conclusion Hsa‐circ‐0052001 functions as a tumor gene in promoting the progression of GC through MAPK pathway, which has provided a promising target for patients with GC. |
| first_indexed | 2024-04-09T20:02:58Z |
| format | Article |
| id | doaj.art-ade9d32502fd45a99d838c7c063e9da7 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-04-09T20:02:58Z |
| publishDate | 2023-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-ade9d32502fd45a99d838c7c063e9da72023-04-02T20:55:00ZengWileyCancer Medicine2045-76342023-03-011267246725710.1002/cam4.5446Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathwayQixuan Xu0Yizhou Yao1Haishun Ni2Jinrong Gu3Xuchao Wang4Linhua Jiang5Bin Wang6Xinguo Zhu7Department of General Surgery The First Affiliated Hospital of Soochow University Suzhou People's Republic of ChinaDepartment of General Surgery The First Affiliated Hospital of Soochow University Suzhou People's Republic of ChinaDepartment of General Surgery The First Affiliated Hospital of Soochow University Suzhou People's Republic of ChinaDepartment of General Surgery The First Affiliated Hospital of Soochow University Suzhou People's Republic of ChinaDepartment of General Surgery The First Affiliated Hospital of Soochow University Suzhou People's Republic of ChinaDepartment of General Surgery The First Affiliated Hospital of Soochow University Suzhou People's Republic of ChinaDepartment of General Surgery The First Affiliated Hospital of Soochow University Suzhou People's Republic of ChinaDepartment of General Surgery The First Affiliated Hospital of Soochow University Suzhou People's Republic of ChinaAbstract Background Gastric cancer (GC) ranks fourth among the causes of death from malignant tumors in the world. Studies have implicated the dysregulation of circRNAs with GC. However, the relationship between hsa‐circ‐0052001 and GC is unclear. Methods In our current study, we assessed the expression levels of hsa‐circ‐0052001 in GC cells and tissues using quantitative real‐time PCR (qPCR). The role of hsa‐circ‐0052001 expression on the proliferation and invasion of GC cells was assessed using in vitro experiments. The role of hsa‐circ‐0052001 on the proliferation of GC cells was also analyzed using in vivo models. The pathways downstream of hsa‐circ‐0052001 were identified using bioinformatics analyses, western blot (WB) assays, and qRT‐PCR. Results We found that compared with normal gastric mucosa epithelial cells and adjacent paracancer tissues, hsa‐circ‐0052001 was overexpressed in GC cells and tissues. Also, the hsa‐circ‐0052001 level was linked to patient clinicopathological characteristics of GC. Cell proliferation and metastatic ability were inhibited in gastric cancer cells when hsa‐circ‐0052001 was knocked down in vitro and cancer growth in vivo. Mechanistically, hsa‐circ‐0052001 promoted the carcinogenesis of GC cells via the MAPK signal pathway. Conclusion Hsa‐circ‐0052001 functions as a tumor gene in promoting the progression of GC through MAPK pathway, which has provided a promising target for patients with GC.https://doi.org/10.1002/cam4.5446circRNAgastric cancerhsa‐circ‐0052001MAPKmiR‐608 |
| spellingShingle | Qixuan Xu Yizhou Yao Haishun Ni Jinrong Gu Xuchao Wang Linhua Jiang Bin Wang Xinguo Zhu Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathway Cancer Medicine circRNA gastric cancer hsa‐circ‐0052001 MAPK miR‐608 |
| title | Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathway |
| title_full | Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathway |
| title_fullStr | Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathway |
| title_full_unstemmed | Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathway |
| title_short | Hsa‐circ‐0052001 promotes gastric cancer cell proliferation and invasion via the MAPK pathway |
| title_sort | hsa circ 0052001 promotes gastric cancer cell proliferation and invasion via the mapk pathway |
| topic | circRNA gastric cancer hsa‐circ‐0052001 MAPK miR‐608 |
| url | https://doi.org/10.1002/cam4.5446 |
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