Potential Antimalarial Activity of Artemether/Lumefantrine/Doxycycline: A Study in Mice Infected with Plasmodium berghei
Antimalarial drug resistance is one of the greatest challenges towards eradicating malaria. Exploring new combination therapies can overcome resistance challenges. The present study examined the antiplasmodial effect of artemether/lumefantrine/doxycycline (A/L/D) on a mouse model infected with Plasm...
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| Format: | Article |
| Language: | English |
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State Islamic University Sunan Kalijaga
2022-02-01
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| Series: | Biology, Medicine & Natural Product Chemistry |
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| Online Access: | https://sciencebiology.org/index.php/BIOMEDICH/article/view/167 |
| _version_ | 1818502797362987008 |
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| author | Udeme Owunari Georgewill Elias Adikwu |
| author_facet | Udeme Owunari Georgewill Elias Adikwu |
| author_sort | Udeme Owunari Georgewill |
| collection | DOAJ |
| description | Antimalarial drug resistance is one of the greatest challenges towards eradicating malaria. Exploring new combination therapies can overcome resistance challenges. The present study examined the antiplasmodial effect of artemether/lumefantrine/doxycycline (A/L/D) on a mouse model infected with Plasmodium berghei. Adult Swiss albino mice (22-30g) intraperitoneally infected with blood containing 1x107 Plasmodium berghei were randomly grouped and orally treated daily with D (2.2 mg/kg), A/L (1.71/13.7 mg/kg) and A/L/D. The negative control was treated daily with normal saline (0.2ml) whereas the positive control was treated daily with chloroquine (CQ) (10mg/kg). After treatment, blood samples were assessed for percentage parasitemia and biochemical parameters. Mice were observed for mean survival time (MST). D, A/L and A/L/D produced significant decreases in percentage parasitemia levels at p<0.05; p<0.01 and p<0.001, respectively when compared to negative control. In the curative test, D, A/L and A/L/D produced 60.4%, 70.3%, and 90.0% parasitemia inhibitions, respectively whereas CQ produced 76.0% parasitemia inhibition. D, A/L, A/L/D and CQ produced 63.2 %, 80.1%, 92.3% and 83.6% parasitemia inhibitions, respectively in the suppressive test. D, A/L, and A/L/D prevented Plasmodium berghei-induced alterations in biochemical parameters by increasing packed cell volume, red blood cells, hemoglobin, and high-density lipoprotein and decreasing white blood cells, total cholesterol, low-density lipoprotein cholesterol, and triglyceride levels significantly at p<0.05 and p<0.01 and p<0.001, respectively when compared to the negative control. A/L/D produced significant antiplasmodial activity therefore, it may be used clinically for the treatment of malaria. |
| first_indexed | 2024-12-10T21:15:14Z |
| format | Article |
| id | doaj.art-adeb8c1dafb1471cb9cade1fc064e11d |
| institution | Directory Open Access Journal |
| issn | 2089-6514 2540-9328 |
| language | English |
| last_indexed | 2024-12-10T21:15:14Z |
| publishDate | 2022-02-01 |
| publisher | State Islamic University Sunan Kalijaga |
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| series | Biology, Medicine & Natural Product Chemistry |
| spelling | doaj.art-adeb8c1dafb1471cb9cade1fc064e11d2022-12-22T01:33:20ZengState Islamic University Sunan KalijagaBiology, Medicine & Natural Product Chemistry2089-65142540-93282022-02-011111610.14421/biomedich.2022.111.1-6123Potential Antimalarial Activity of Artemether/Lumefantrine/Doxycycline: A Study in Mice Infected with Plasmodium bergheiUdeme Owunari Georgewill0Elias Adikwu1Department of Pharmacology, Faculty of Basic Clinical Sciences, University of Port Harcourt, Rivers StateDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, Niger Delta University, Bayelsa StateAntimalarial drug resistance is one of the greatest challenges towards eradicating malaria. Exploring new combination therapies can overcome resistance challenges. The present study examined the antiplasmodial effect of artemether/lumefantrine/doxycycline (A/L/D) on a mouse model infected with Plasmodium berghei. Adult Swiss albino mice (22-30g) intraperitoneally infected with blood containing 1x107 Plasmodium berghei were randomly grouped and orally treated daily with D (2.2 mg/kg), A/L (1.71/13.7 mg/kg) and A/L/D. The negative control was treated daily with normal saline (0.2ml) whereas the positive control was treated daily with chloroquine (CQ) (10mg/kg). After treatment, blood samples were assessed for percentage parasitemia and biochemical parameters. Mice were observed for mean survival time (MST). D, A/L and A/L/D produced significant decreases in percentage parasitemia levels at p<0.05; p<0.01 and p<0.001, respectively when compared to negative control. In the curative test, D, A/L and A/L/D produced 60.4%, 70.3%, and 90.0% parasitemia inhibitions, respectively whereas CQ produced 76.0% parasitemia inhibition. D, A/L, A/L/D and CQ produced 63.2 %, 80.1%, 92.3% and 83.6% parasitemia inhibitions, respectively in the suppressive test. D, A/L, and A/L/D prevented Plasmodium berghei-induced alterations in biochemical parameters by increasing packed cell volume, red blood cells, hemoglobin, and high-density lipoprotein and decreasing white blood cells, total cholesterol, low-density lipoprotein cholesterol, and triglyceride levels significantly at p<0.05 and p<0.01 and p<0.001, respectively when compared to the negative control. A/L/D produced significant antiplasmodial activity therefore, it may be used clinically for the treatment of malaria.https://sciencebiology.org/index.php/BIOMEDICH/article/view/167antiplasmodialartemethertetracyclineantimalariallumefantrineplasmodium berghei |
| spellingShingle | Udeme Owunari Georgewill Elias Adikwu Potential Antimalarial Activity of Artemether/Lumefantrine/Doxycycline: A Study in Mice Infected with Plasmodium berghei Biology, Medicine & Natural Product Chemistry antiplasmodial artemether tetracycline antimalarial lumefantrine plasmodium berghei |
| title | Potential Antimalarial Activity of Artemether/Lumefantrine/Doxycycline: A Study in Mice Infected with Plasmodium berghei |
| title_full | Potential Antimalarial Activity of Artemether/Lumefantrine/Doxycycline: A Study in Mice Infected with Plasmodium berghei |
| title_fullStr | Potential Antimalarial Activity of Artemether/Lumefantrine/Doxycycline: A Study in Mice Infected with Plasmodium berghei |
| title_full_unstemmed | Potential Antimalarial Activity of Artemether/Lumefantrine/Doxycycline: A Study in Mice Infected with Plasmodium berghei |
| title_short | Potential Antimalarial Activity of Artemether/Lumefantrine/Doxycycline: A Study in Mice Infected with Plasmodium berghei |
| title_sort | potential antimalarial activity of artemether lumefantrine doxycycline a study in mice infected with plasmodium berghei |
| topic | antiplasmodial artemether tetracycline antimalarial lumefantrine plasmodium berghei |
| url | https://sciencebiology.org/index.php/BIOMEDICH/article/view/167 |
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