Slc43a3 is a regulator of free fatty acid flux1
Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known fu...
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Format: | Article |
Language: | English |
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Elsevier
2020-05-01
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Series: | Journal of Lipid Research |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520436223 |
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author | Kathrin B. Hasbargen Wen-Jun Shen Yiqiang Zhang Xiaoming Hou Wei Wang Qui Shuo David A. Bernlohr Salman Azhar Fredric B. Kraemer |
author_facet | Kathrin B. Hasbargen Wen-Jun Shen Yiqiang Zhang Xiaoming Hou Wei Wang Qui Shuo David A. Bernlohr Salman Azhar Fredric B. Kraemer |
author_sort | Kathrin B. Hasbargen |
collection | DOAJ |
description | Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known function were screened in subcutaneous adipose samples from obese patients before and after bariatric surgery using branched DNA methodology. Among the 33 solute carrier (SLC) transporter family members screened, the expression of 14 members showed significant changes before and after bariatric surgery. One of them, Slc43a3, increased about 2.5-fold after bariatric surgery. Further investigation demonstrated that Slc43a3 is highly expressed in murine adipose tissue and induced during adipocyte differentiation in primary preadipocytes and in OP9 cells. Knockdown of Slc43a3 with siRNA in differentiated OP9 adipocytes reduced both basal and forskolin-stimulated FA efflux, while also increasing FA uptake and lipid droplet accumulation. In contrast, overexpression of Slc43a3 decreased FA uptake in differentiated OP9 cells and resulted in decreased lipid droplet accumulation. Therefore, Slc43a3 seems to regulate FA flux in adipocytes, functioning as a positive regulator of FA efflux and as a negative regulator of FA uptake. |
first_indexed | 2024-12-17T12:27:16Z |
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id | doaj.art-adee7867d43c4ae8b513887121686c5d |
institution | Directory Open Access Journal |
issn | 0022-2275 |
language | English |
last_indexed | 2024-12-17T12:27:16Z |
publishDate | 2020-05-01 |
publisher | Elsevier |
record_format | Article |
series | Journal of Lipid Research |
spelling | doaj.art-adee7867d43c4ae8b513887121686c5d2022-12-21T21:48:41ZengElsevierJournal of Lipid Research0022-22752020-05-01615734745Slc43a3 is a regulator of free fatty acid flux1Kathrin B. Hasbargen0Wen-Jun Shen1Yiqiang Zhang2Xiaoming Hou3Wei Wang4Qui Shuo5David A. Bernlohr6Salman Azhar7Fredric B. Kraemer8Division of Endocrinology, Gerontology, and Metabolism,Stanford University, Stanford, CA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CA; Department of Biochemistry,Changzhi Medical College, ShanXi, ChinaDivision of Endocrinology, Gerontology, and Metabolism,Stanford University, Stanford, CA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CADivision of Endocrinology, Gerontology, and Metabolism,Stanford University, Stanford, CA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CA; Department of Biochemistry,Changzhi Medical College, ShanXi, ChinaDivision of Endocrinology, Gerontology, and Metabolism,Stanford University, Stanford, CA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CADivision of Endocrinology, Gerontology, and Metabolism,Stanford University, Stanford, CA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CA; Department of Endocrinology,Peking University First Hospital, Beijing, ChinaVeterans Affairs Palo Alto Health Care System, Palo Alto, CADepartment of Biochemistry, Molecular Biology, and Biophysics,University of Minnesota, Minneapolis, MNDivision of Endocrinology, Gerontology, and Metabolism,Stanford University, Stanford, CA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CATo whom correspondence should be addressed; Division of Endocrinology, Gerontology, and Metabolism,Stanford University, Stanford, CA; Veterans Affairs Palo Alto Health Care System, Palo Alto, CA; Stanford Diabetes Research Center, Stanford, CA; To whom correspondence should be addressedAdipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known function were screened in subcutaneous adipose samples from obese patients before and after bariatric surgery using branched DNA methodology. Among the 33 solute carrier (SLC) transporter family members screened, the expression of 14 members showed significant changes before and after bariatric surgery. One of them, Slc43a3, increased about 2.5-fold after bariatric surgery. Further investigation demonstrated that Slc43a3 is highly expressed in murine adipose tissue and induced during adipocyte differentiation in primary preadipocytes and in OP9 cells. Knockdown of Slc43a3 with siRNA in differentiated OP9 adipocytes reduced both basal and forskolin-stimulated FA efflux, while also increasing FA uptake and lipid droplet accumulation. In contrast, overexpression of Slc43a3 decreased FA uptake in differentiated OP9 cells and resulted in decreased lipid droplet accumulation. Therefore, Slc43a3 seems to regulate FA flux in adipocytes, functioning as a positive regulator of FA efflux and as a negative regulator of FA uptake.http://www.sciencedirect.com/science/article/pii/S0022227520436223lipid dropletadipose metabolismmembrane transportersfatty acid uptakefatty acid effluxsolute carrier family 43 member 3 |
spellingShingle | Kathrin B. Hasbargen Wen-Jun Shen Yiqiang Zhang Xiaoming Hou Wei Wang Qui Shuo David A. Bernlohr Salman Azhar Fredric B. Kraemer Slc43a3 is a regulator of free fatty acid flux1 Journal of Lipid Research lipid droplet adipose metabolism membrane transporters fatty acid uptake fatty acid efflux solute carrier family 43 member 3 |
title | Slc43a3 is a regulator of free fatty acid flux1 |
title_full | Slc43a3 is a regulator of free fatty acid flux1 |
title_fullStr | Slc43a3 is a regulator of free fatty acid flux1 |
title_full_unstemmed | Slc43a3 is a regulator of free fatty acid flux1 |
title_short | Slc43a3 is a regulator of free fatty acid flux1 |
title_sort | slc43a3 is a regulator of free fatty acid flux1 |
topic | lipid droplet adipose metabolism membrane transporters fatty acid uptake fatty acid efflux solute carrier family 43 member 3 |
url | http://www.sciencedirect.com/science/article/pii/S0022227520436223 |
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