Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In Planta
The ability of the Pseudomonas syringae pv. phaseolicola harpin (HrpZPsph) to elicit hypersensitive response was investigated in three Nicotiana genotypes. The hrpZPsph gene was placed under chemical regulation (tetracycline induction) in TetR+ Nicotiana tabacum cv. Wisconsin 38 (W38) or was transie...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2000-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.12.1366 |
| _version_ | 1818915186889719808 |
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| author | Anastasia P. Tampakaki Nickolas J. Panopoulos |
| author_facet | Anastasia P. Tampakaki Nickolas J. Panopoulos |
| author_sort | Anastasia P. Tampakaki |
| collection | DOAJ |
| description | The ability of the Pseudomonas syringae pv. phaseolicola harpin (HrpZPsph) to elicit hypersensitive response was investigated in three Nicotiana genotypes. The hrpZPsph gene was placed under chemical regulation (tetracycline induction) in TetR+ Nicotiana tabacum cv. Wisconsin 38 (W38) or was transiently expressed in N. benthamiana following infection with a PVX-derived vector and in three Nicotiana genotypes by agroinfiltration. The constructs were designed to express either the canonical form of harpin (HrpZPsph) or an N-terminally extended version of the protein carrying the signal peptide portion of the tobacco pathogenesis-related protein PR1a (SP-HrpZPsph). Stable transformants of N. tabacum cv. W38 did not develop necrosis upon induction with tetracycline, probably as a result of insufficient harpin accumulation. In contrast, N. benthamiana plants infected with the PVX constructs produced high concentrations of harpin in biologically active form, but only those expressing the secretable form of harpin developed necrotic symptoms. These symptoms were less severe than those caused by PVX∷avrPto; however, they were accompanied by induction of hsr203J, a hypersensitive response-specific gene transcript. These results suggest that the plant cellular receptor(s) for harpin is extracellular. |
| first_indexed | 2024-12-19T23:58:17Z |
| format | Article |
| id | doaj.art-adeebed8250b441ebf601496f40ce57c |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:58:17Z |
| publishDate | 2000-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-adeebed8250b441ebf601496f40ce57c2022-12-21T20:00:55ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-12-0113121366137410.1094/MPMI.2000.13.12.1366Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In PlantaAnastasia P. TampakakiNickolas J. PanopoulosThe ability of the Pseudomonas syringae pv. phaseolicola harpin (HrpZPsph) to elicit hypersensitive response was investigated in three Nicotiana genotypes. The hrpZPsph gene was placed under chemical regulation (tetracycline induction) in TetR+ Nicotiana tabacum cv. Wisconsin 38 (W38) or was transiently expressed in N. benthamiana following infection with a PVX-derived vector and in three Nicotiana genotypes by agroinfiltration. The constructs were designed to express either the canonical form of harpin (HrpZPsph) or an N-terminally extended version of the protein carrying the signal peptide portion of the tobacco pathogenesis-related protein PR1a (SP-HrpZPsph). Stable transformants of N. tabacum cv. W38 did not develop necrosis upon induction with tetracycline, probably as a result of insufficient harpin accumulation. In contrast, N. benthamiana plants infected with the PVX constructs produced high concentrations of harpin in biologically active form, but only those expressing the secretable form of harpin developed necrotic symptoms. These symptoms were less severe than those caused by PVX∷avrPto; however, they were accompanied by induction of hsr203J, a hypersensitive response-specific gene transcript. These results suggest that the plant cellular receptor(s) for harpin is extracellular.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.12.1366bacterial hypersensitive response elicitorbean halo blight pathogenhrp-hrc genestype III secreted protein |
| spellingShingle | Anastasia P. Tampakaki Nickolas J. Panopoulos Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In Planta Molecular Plant-Microbe Interactions bacterial hypersensitive response elicitor bean halo blight pathogen hrp-hrc genes type III secreted protein |
| title | Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In Planta |
| title_full | Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In Planta |
| title_fullStr | Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In Planta |
| title_full_unstemmed | Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In Planta |
| title_short | Elicitation of Hypersensitive Cell Death by Extracellularly Targeted HrpZPsph Produced In Planta |
| title_sort | elicitation of hypersensitive cell death by extracellularly targeted hrpzpsph produced in planta |
| topic | bacterial hypersensitive response elicitor bean halo blight pathogen hrp-hrc genes type III secreted protein |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.12.1366 |
| work_keys_str_mv | AT anastasiaptampakaki elicitationofhypersensitivecelldeathbyextracellularlytargetedhrpzpsphproducedinplanta AT nickolasjpanopoulos elicitationofhypersensitivecelldeathbyextracellularlytargetedhrpzpsphproducedinplanta |