A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery
Purpose: To develop a simple method to extract the whole apolipoproteins (apo) including apoA-I in native high density lipoproteins (HDLs) and prepare discoidal Tanshinone IIA-loaded reconstituted HDL (TA-rHDLs) as a dual functional drug delivery system with plaque-site target and therapeutic promis...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-05-01
|
| Series: | Journal of Pharmacy & Pharmaceutical Sciences |
| Online Access: | https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/24334 |
| _version_ | 1797719585761263616 |
|---|---|
| author | Wenli Zhang Ji Wang Junting Jia Liang Chen Zimei Wu Jianping Liu |
| author_facet | Wenli Zhang Ji Wang Junting Jia Liang Chen Zimei Wu Jianping Liu |
| author_sort | Wenli Zhang |
| collection | DOAJ |
| description | Purpose: To develop a simple method to extract the whole apolipoproteins (apo) including apoA-I in native high density lipoproteins (HDLs) and prepare discoidal Tanshinone IIA-loaded reconstituted HDL (TA-rHDLs) as a dual functional drug delivery system with plaque-site target and therapeutic promises in atherosclerotic lesions. Methods: A method based on isoelectric precipitation coupled with organic solvent precipitation was developed to isolate the whole apolipoproteins (apos). TA-rHDLs were prepared by incubating the resultant apos with liposomes and the incubation conditions were optimized using fluorescence quenching experiment. TA-rHDLs were characterized in terms of size, zeta potential, morphology, interaction between lipid and apos, safety, and bionic function. Results: The extraction results showed that the yield of the HDL apos was 82.4%, with 59% being apoA-I type, similar ratio of apoA-I in the native apos. TA-rHDL prepared were disc-like with an average diameter of 157.6 ± 4.8 nm, zeta potential of -20.90 ± 0.15 mV, and entrapment efficiency of (90.13 ± 1.4) %. The interaction between the lipids and apos was electrostatic and hydrophobic force and was associated with amino acid sequence. Haemolysis and cytotoxicity assays showed good biocompatibility of TA-rHDL. Sterol efflux assay from macrophages mediated by TA-rHDLs and structure remodeling behavior from discs to spheres proved that TA-rHDL could resemble the biological activity of native nascent HDL irrespective of the size. Conclusions: The simple approach to isolate apos may provide a convenient and economical resource to support the development of rHDL as a potential targeting nanocarrier for lipophilic cardiovascular drugs.
This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page. |
| first_indexed | 2024-03-12T09:08:10Z |
| format | Article |
| id | doaj.art-adf1a8d98b504495934cbc0bbae1cf5c |
| institution | Directory Open Access Journal |
| issn | 1482-1826 |
| language | English |
| last_indexed | 2024-03-12T09:08:10Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmaceutical Sciences |
| spelling | doaj.art-adf1a8d98b504495934cbc0bbae1cf5c2023-09-02T15:12:46ZengFrontiers Media S.A.Journal of Pharmacy & Pharmaceutical Sciences1482-18262015-05-0118210.18433/J3531XA Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug DeliveryWenli Zhang0Ji Wang1Junting Jia2Liang Chen3Zimei Wu4Jianping Liu5Department of Pharmaceutics, China Pharmaceutical University, Nanjing, PR ChinaDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing, PR ChinaDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing, PR ChinaDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing, PR ChinaSchool of Pharmacy, University of Auckland, Private Bag, Auckland, New ZealandDepartment of Pharmaceutics, China Pharmaceutical University, Nanjing, PR ChinaPurpose: To develop a simple method to extract the whole apolipoproteins (apo) including apoA-I in native high density lipoproteins (HDLs) and prepare discoidal Tanshinone IIA-loaded reconstituted HDL (TA-rHDLs) as a dual functional drug delivery system with plaque-site target and therapeutic promises in atherosclerotic lesions. Methods: A method based on isoelectric precipitation coupled with organic solvent precipitation was developed to isolate the whole apolipoproteins (apos). TA-rHDLs were prepared by incubating the resultant apos with liposomes and the incubation conditions were optimized using fluorescence quenching experiment. TA-rHDLs were characterized in terms of size, zeta potential, morphology, interaction between lipid and apos, safety, and bionic function. Results: The extraction results showed that the yield of the HDL apos was 82.4%, with 59% being apoA-I type, similar ratio of apoA-I in the native apos. TA-rHDL prepared were disc-like with an average diameter of 157.6 ± 4.8 nm, zeta potential of -20.90 ± 0.15 mV, and entrapment efficiency of (90.13 ± 1.4) %. The interaction between the lipids and apos was electrostatic and hydrophobic force and was associated with amino acid sequence. Haemolysis and cytotoxicity assays showed good biocompatibility of TA-rHDL. Sterol efflux assay from macrophages mediated by TA-rHDLs and structure remodeling behavior from discs to spheres proved that TA-rHDL could resemble the biological activity of native nascent HDL irrespective of the size. Conclusions: The simple approach to isolate apos may provide a convenient and economical resource to support the development of rHDL as a potential targeting nanocarrier for lipophilic cardiovascular drugs. This article is open to POST-PUBLICATION REVIEW. Registered readers (see “For Readers”) may comment by clicking on ABSTRACT on the issue’s contents page.https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/24334 |
| spellingShingle | Wenli Zhang Ji Wang Junting Jia Liang Chen Zimei Wu Jianping Liu A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery Journal of Pharmacy & Pharmaceutical Sciences |
| title | A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery |
| title_full | A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery |
| title_fullStr | A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery |
| title_full_unstemmed | A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery |
| title_short | A Simple Method to Extract Whole Apolipoproteins for the Preparation of Discoidal Recombined High Density Lipoproteins as Bionic Nanocarriers for Drug Delivery |
| title_sort | simple method to extract whole apolipoproteins for the preparation of discoidal recombined high density lipoproteins as bionic nanocarriers for drug delivery |
| url | https://journals.library.ualberta.ca/jpps/index.php/JPPS/article/view/24334 |
| work_keys_str_mv | AT wenlizhang asimplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT jiwang asimplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT juntingjia asimplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT liangchen asimplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT zimeiwu asimplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT jianpingliu asimplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT wenlizhang simplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT jiwang simplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT juntingjia simplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT liangchen simplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT zimeiwu simplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery AT jianpingliu simplemethodtoextractwholeapolipoproteinsforthepreparationofdiscoidalrecombinedhighdensitylipoproteinsasbionicnanocarriersfordrugdelivery |