Plasma Branched-Chain Amino Acid Concentrations and Glucose Homeostasis in Kidney Transplant Recipients and Candidates
Background: Post-transplant diabetes mellitus (PTDM) encompasses new-onset and previously unrecognized type 2 diabetes. Kidney failure masks type 2 diabetes. Branched-chain amino acids (BCAA) are closely associated with glucose metabolism. Therefore, understanding BCAA metabolism both in kidney fail...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SAGE Publishing
2023-04-01
|
Series: | Canadian Journal of Kidney Health and Disease |
Online Access: | https://doi.org/10.1177/20543581231168085 |
_version_ | 1797844207723872256 |
---|---|
author | G. V. Ramesh Prasad M. M. Nash W. Yuan D. Beriault M. Yazdanpanah P. W. Connelly |
author_facet | G. V. Ramesh Prasad M. M. Nash W. Yuan D. Beriault M. Yazdanpanah P. W. Connelly |
author_sort | G. V. Ramesh Prasad |
collection | DOAJ |
description | Background: Post-transplant diabetes mellitus (PTDM) encompasses new-onset and previously unrecognized type 2 diabetes. Kidney failure masks type 2 diabetes. Branched-chain amino acids (BCAA) are closely associated with glucose metabolism. Therefore, understanding BCAA metabolism both in kidney failure and after kidney transplantation may inform PTDM mechanisms. Objective: To understand the impact of present or absent kidney function on plasma BCAA concentrations. Design: Cross-sectional study of kidney transplant recipients and kidney transplant candidates. Setting: Large kidney transplant center in Toronto, Canada. Measurements: We measured plasma BCAA and aromatic amino acid (AAA) concentrations in 45 pre-kidney transplant candidates (15 with type 2 diabetes, 30 without type 2 diabetes) and 45 post-kidney transplant recipients (15 PTDM, 30 non-PTDM), along with insulin resistance and sensitivity by 75 g oral glucose loading for those in each group without type 2 diabetes. Methods: Plasma AA concentrations were analyzed using MassChrom AA Analysis and compared between groups. The insulin sensitivity for oral glucose tolerance tests or Matsuda index (a measure of whole-body insulin resistance), Homeostatic Model Assessment for Insulin Resistance (a measure of hepatic insulin resistance), and Insulin Secretion-Sensitivity Index-2 (ISSI-2, a measure of pancreatic β-cell response) was calculated from fasting insulin and glucose concentrations, and compared with BCAA concentrations. Results: Each BCAA concentration was higher in post-transplant subjects than pre-transplant subjects ( P < .001 for leucine, isoleucine, valine). In post-transplant subjects, each BCAA concentration was higher in PTDM versus non-PTDM (odds ratio for PTDM 3-4 per 1 SD increase in BCAA concentration, P < .001 for each). Tyrosine concentrations were also higher in post-transplant subjects than pre-transplant subjects, but tyrosine did not differ by PTDM status. By contrast, neither BCAA nor AAA concentrations were different in pre-transplant subjects with or without type 2 diabetes. Whole-body insulin resistance, hepatic insulin resistance, and pancreatic β-cell response did not differ between nondiabetic post-transplant and pre-transplant subjects. Branched-chain amino acid concentrations correlated with the Matsuda index and Homeostatic Model Assessment for Insulin Resistance ( P < .05 for each) only in nondiabetic post-transplant subjects—not in nondiabetic pre-transplant subjects. Branched-chain amino acid concentrations did not correlate with ISSI-2 in either pre-transplant or post-transplant subjects. Limitations: The sample size was small, and subjects were not studied prospectively for the development of type 2 diabetes. Conclusions: Plasma BCAA concentrations are higher post-transplant in type 2 diabetic states, but do not differ by diabetes status in the presence of kidney failure. The association of BCAA with measures of hepatic insulin resistance among nondiabetic post-transplant patients is consistent with impaired BCAA metabolism as a characteristic of kidney transplantation. |
first_indexed | 2024-04-09T17:18:37Z |
format | Article |
id | doaj.art-adf2d362bb374078ad5fa6a8b53e0a82 |
institution | Directory Open Access Journal |
issn | 2054-3581 |
language | English |
last_indexed | 2024-04-09T17:18:37Z |
publishDate | 2023-04-01 |
publisher | SAGE Publishing |
record_format | Article |
series | Canadian Journal of Kidney Health and Disease |
spelling | doaj.art-adf2d362bb374078ad5fa6a8b53e0a822023-04-19T07:33:26ZengSAGE PublishingCanadian Journal of Kidney Health and Disease2054-35812023-04-011010.1177/20543581231168085Plasma Branched-Chain Amino Acid Concentrations and Glucose Homeostasis in Kidney Transplant Recipients and CandidatesG. V. Ramesh Prasad0M. M. Nash1W. Yuan2D. Beriault3M. Yazdanpanah4P. W. Connelly5Kidney Transplant Program, St. Michael’s Hospital, Toronto, ON, CanadaKidney Transplant Program, St. Michael’s Hospital, Toronto, ON, CanadaKidney Transplant Program, St. Michael’s Hospital, Toronto, ON, CanadaDepartment of Laboratory Medicine, University of Toronto, ON, CanadaDepartment of Laboratory Medicine, University of Toronto, ON, CanadaDepartment of Medicine, University of Toronto, ON, CanadaBackground: Post-transplant diabetes mellitus (PTDM) encompasses new-onset and previously unrecognized type 2 diabetes. Kidney failure masks type 2 diabetes. Branched-chain amino acids (BCAA) are closely associated with glucose metabolism. Therefore, understanding BCAA metabolism both in kidney failure and after kidney transplantation may inform PTDM mechanisms. Objective: To understand the impact of present or absent kidney function on plasma BCAA concentrations. Design: Cross-sectional study of kidney transplant recipients and kidney transplant candidates. Setting: Large kidney transplant center in Toronto, Canada. Measurements: We measured plasma BCAA and aromatic amino acid (AAA) concentrations in 45 pre-kidney transplant candidates (15 with type 2 diabetes, 30 without type 2 diabetes) and 45 post-kidney transplant recipients (15 PTDM, 30 non-PTDM), along with insulin resistance and sensitivity by 75 g oral glucose loading for those in each group without type 2 diabetes. Methods: Plasma AA concentrations were analyzed using MassChrom AA Analysis and compared between groups. The insulin sensitivity for oral glucose tolerance tests or Matsuda index (a measure of whole-body insulin resistance), Homeostatic Model Assessment for Insulin Resistance (a measure of hepatic insulin resistance), and Insulin Secretion-Sensitivity Index-2 (ISSI-2, a measure of pancreatic β-cell response) was calculated from fasting insulin and glucose concentrations, and compared with BCAA concentrations. Results: Each BCAA concentration was higher in post-transplant subjects than pre-transplant subjects ( P < .001 for leucine, isoleucine, valine). In post-transplant subjects, each BCAA concentration was higher in PTDM versus non-PTDM (odds ratio for PTDM 3-4 per 1 SD increase in BCAA concentration, P < .001 for each). Tyrosine concentrations were also higher in post-transplant subjects than pre-transplant subjects, but tyrosine did not differ by PTDM status. By contrast, neither BCAA nor AAA concentrations were different in pre-transplant subjects with or without type 2 diabetes. Whole-body insulin resistance, hepatic insulin resistance, and pancreatic β-cell response did not differ between nondiabetic post-transplant and pre-transplant subjects. Branched-chain amino acid concentrations correlated with the Matsuda index and Homeostatic Model Assessment for Insulin Resistance ( P < .05 for each) only in nondiabetic post-transplant subjects—not in nondiabetic pre-transplant subjects. Branched-chain amino acid concentrations did not correlate with ISSI-2 in either pre-transplant or post-transplant subjects. Limitations: The sample size was small, and subjects were not studied prospectively for the development of type 2 diabetes. Conclusions: Plasma BCAA concentrations are higher post-transplant in type 2 diabetic states, but do not differ by diabetes status in the presence of kidney failure. The association of BCAA with measures of hepatic insulin resistance among nondiabetic post-transplant patients is consistent with impaired BCAA metabolism as a characteristic of kidney transplantation.https://doi.org/10.1177/20543581231168085 |
spellingShingle | G. V. Ramesh Prasad M. M. Nash W. Yuan D. Beriault M. Yazdanpanah P. W. Connelly Plasma Branched-Chain Amino Acid Concentrations and Glucose Homeostasis in Kidney Transplant Recipients and Candidates Canadian Journal of Kidney Health and Disease |
title | Plasma Branched-Chain Amino Acid Concentrations and Glucose Homeostasis in Kidney Transplant Recipients and Candidates |
title_full | Plasma Branched-Chain Amino Acid Concentrations and Glucose Homeostasis in Kidney Transplant Recipients and Candidates |
title_fullStr | Plasma Branched-Chain Amino Acid Concentrations and Glucose Homeostasis in Kidney Transplant Recipients and Candidates |
title_full_unstemmed | Plasma Branched-Chain Amino Acid Concentrations and Glucose Homeostasis in Kidney Transplant Recipients and Candidates |
title_short | Plasma Branched-Chain Amino Acid Concentrations and Glucose Homeostasis in Kidney Transplant Recipients and Candidates |
title_sort | plasma branched chain amino acid concentrations and glucose homeostasis in kidney transplant recipients and candidates |
url | https://doi.org/10.1177/20543581231168085 |
work_keys_str_mv | AT gvrameshprasad plasmabranchedchainaminoacidconcentrationsandglucosehomeostasisinkidneytransplantrecipientsandcandidates AT mmnash plasmabranchedchainaminoacidconcentrationsandglucosehomeostasisinkidneytransplantrecipientsandcandidates AT wyuan plasmabranchedchainaminoacidconcentrationsandglucosehomeostasisinkidneytransplantrecipientsandcandidates AT dberiault plasmabranchedchainaminoacidconcentrationsandglucosehomeostasisinkidneytransplantrecipientsandcandidates AT myazdanpanah plasmabranchedchainaminoacidconcentrationsandglucosehomeostasisinkidneytransplantrecipientsandcandidates AT pwconnelly plasmabranchedchainaminoacidconcentrationsandglucosehomeostasisinkidneytransplantrecipientsandcandidates |