Rapid determination of serum albumin partition coefficients using affinity chromatography
Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target side are derived from nominal concentrations....
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| Format: | Article |
| Language: | English |
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Elsevier
2022-10-01
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| Series: | Environmental Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666765722001193 |
| _version_ | 1798013805345636352 |
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| author | Nadin Ulrich Alexander Böhme |
| author_facet | Nadin Ulrich Alexander Böhme |
| author_sort | Nadin Ulrich |
| collection | DOAJ |
| description | Sorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target side are derived from nominal concentrations. Sorption to serum albumin is often characterized by the serum albumin-water partition coefficient KSA/W, which is traditionally determined using dialysis experiments. However, dialysis approaches are time-consuming and require a large amount of pure protein. Thus, we investigated affinity chromatography as a fast alternative to characterize the sorption of chemicals to serum albumin. To this end, the application of two types of serum albumin columns (bovine vs. human), different temperature settings (30°C vs. 37°C), and the impact of 2-propanol as modifier in the mobile phase were evaluated. Moreover, a linear regression model was calibrated with 49 experimentally determined log KSA/W values from the literature to predict sorption to serum albumin directly from the retention factor log kHSA.In addition, we tested a prediction approach based on linear solvation energy relationships and compared the two procedures. Further, we compiled a dataset of log kHSA values for 378 chemicals, which we classified according to their ionization state and structure, and according to environmentally relevant classes like persistent, mobile, and toxic chemicals, pesticides, and plastic additives. There was no specific trend observed for particular classes of chemicals, which means that there is a demand for determining the sorption to serum albumin for these chemicals for the correction of the effect concentration in bioassays, e.g., and to assess bioaccumulation in higher tier organisms such as fish or rodents. |
| first_indexed | 2024-04-11T15:07:22Z |
| format | Article |
| id | doaj.art-adf488b4b80b4395acf132adf6ab3d47 |
| institution | Directory Open Access Journal |
| issn | 2666-7657 |
| language | English |
| last_indexed | 2024-04-11T15:07:22Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environmental Advances |
| spelling | doaj.art-adf488b4b80b4395acf132adf6ab3d472022-12-22T04:16:44ZengElsevierEnvironmental Advances2666-76572022-10-019100284Rapid determination of serum albumin partition coefficients using affinity chromatographyNadin Ulrich0Alexander Böhme1Corresponding author.; Department of Ecological Chemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, GermanyDepartment of Ecological Chemistry, Helmholtz Centre for Environmental Research - UFZ, Permoserstrasse 15, Leipzig D-04318, GermanySorption to serum albumin is essential for characterizing the distribution of chemicals in organisms. It impacts the concentration of the freely dissolved chemical in vivo and in vitro, and needs to be considered when effect concentrations at the target side are derived from nominal concentrations. Sorption to serum albumin is often characterized by the serum albumin-water partition coefficient KSA/W, which is traditionally determined using dialysis experiments. However, dialysis approaches are time-consuming and require a large amount of pure protein. Thus, we investigated affinity chromatography as a fast alternative to characterize the sorption of chemicals to serum albumin. To this end, the application of two types of serum albumin columns (bovine vs. human), different temperature settings (30°C vs. 37°C), and the impact of 2-propanol as modifier in the mobile phase were evaluated. Moreover, a linear regression model was calibrated with 49 experimentally determined log KSA/W values from the literature to predict sorption to serum albumin directly from the retention factor log kHSA.In addition, we tested a prediction approach based on linear solvation energy relationships and compared the two procedures. Further, we compiled a dataset of log kHSA values for 378 chemicals, which we classified according to their ionization state and structure, and according to environmentally relevant classes like persistent, mobile, and toxic chemicals, pesticides, and plastic additives. There was no specific trend observed for particular classes of chemicals, which means that there is a demand for determining the sorption to serum albumin for these chemicals for the correction of the effect concentration in bioassays, e.g., and to assess bioaccumulation in higher tier organisms such as fish or rodents.http://www.sciencedirect.com/science/article/pii/S2666765722001193Serum albumin partition coefficientAffinity chromatographyLinear solvation energy relationshipLSER |
| spellingShingle | Nadin Ulrich Alexander Böhme Rapid determination of serum albumin partition coefficients using affinity chromatography Environmental Advances Serum albumin partition coefficient Affinity chromatography Linear solvation energy relationship LSER |
| title | Rapid determination of serum albumin partition coefficients using affinity chromatography |
| title_full | Rapid determination of serum albumin partition coefficients using affinity chromatography |
| title_fullStr | Rapid determination of serum albumin partition coefficients using affinity chromatography |
| title_full_unstemmed | Rapid determination of serum albumin partition coefficients using affinity chromatography |
| title_short | Rapid determination of serum albumin partition coefficients using affinity chromatography |
| title_sort | rapid determination of serum albumin partition coefficients using affinity chromatography |
| topic | Serum albumin partition coefficient Affinity chromatography Linear solvation energy relationship LSER |
| url | http://www.sciencedirect.com/science/article/pii/S2666765722001193 |
| work_keys_str_mv | AT nadinulrich rapiddeterminationofserumalbuminpartitioncoefficientsusingaffinitychromatography AT alexanderbohme rapiddeterminationofserumalbuminpartitioncoefficientsusingaffinitychromatography |