Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte

Abstract Aim: The influence of three different culture media on the growth and biochemical composition of Ankistrodemsus gracilis microalgae is evaluated. Methods Microalgae were placed in a bath culture during 28 days, with inorganic fertilizer (NPK) and macrophyte Eichhornia crassipes as culture...

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Main Authors: Lúcia Helena Sipaúba-Tavares, Alexandra Maria Donadon Lusser Segali, Flavia Almeida Berchielli-Morais, Bruno Scardoeli-Truzzi
Format: Article
Language:English
Published: Associação Brasileira de Limnologia 2017-07-01
Series:Acta Limnologica Brasiliensia
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2017000100305&lng=en&tlng=en
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author Lúcia Helena Sipaúba-Tavares
Alexandra Maria Donadon Lusser Segali
Flavia Almeida Berchielli-Morais
Bruno Scardoeli-Truzzi
author_facet Lúcia Helena Sipaúba-Tavares
Alexandra Maria Donadon Lusser Segali
Flavia Almeida Berchielli-Morais
Bruno Scardoeli-Truzzi
author_sort Lúcia Helena Sipaúba-Tavares
collection DOAJ
description Abstract Aim: The influence of three different culture media on the growth and biochemical composition of Ankistrodemsus gracilis microalgae is evaluated. Methods Microalgae were placed in a bath culture during 28 days, with inorganic fertilizer (NPK) and macrophyte Eichhornia crassipes as culture medium to compare the growth of microalgae and commercial medium CHU12. Protein and lipid contents, water conditions of the culture medium and physiological parameters were assessed weekly. Results Growth rate, macro-minerals, micro-nutrients and nutritional values were similar to commercial culture medium in the media with inorganic fertilizer and macrophyte. The biological indexes of microalgae were not influenced by weekly differences during the study period. Although lipid contents, nitrogen and growth rate of A. gracilis were higher in inorganic fertilizer and macrophyte media; Mn, B, Cu, S, dry matter, ash content, cell volume, TOC and total length were similar among the media used. The same high cell density 25 x 105 cells.mL-1 was obtained in NPK and in CHU12 medium respectively after 11 and 14 days cultivation. Conclusion Culture media inorganic fertilizer (NPK) and macrophyte (Eichhornia crassipes), were adequate and may replace the commercial medium CHU12 for the cultivation of microalgae A. gracilis, resulting in high nutritional composition, high biomass, high nutrients and low-cost.
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spelling doaj.art-adf4dc3c22d2490b8696a189702dda212022-12-22T01:33:08ZengAssociação Brasileira de LimnologiaActa Limnologica Brasiliensia2179-975X2017-07-0129010.1590/s2179-975x3916S2179-975X2017000100305Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyteLúcia Helena Sipaúba-TavaresAlexandra Maria Donadon Lusser SegaliFlavia Almeida Berchielli-MoraisBruno Scardoeli-TruzziAbstract Aim: The influence of three different culture media on the growth and biochemical composition of Ankistrodemsus gracilis microalgae is evaluated. Methods Microalgae were placed in a bath culture during 28 days, with inorganic fertilizer (NPK) and macrophyte Eichhornia crassipes as culture medium to compare the growth of microalgae and commercial medium CHU12. Protein and lipid contents, water conditions of the culture medium and physiological parameters were assessed weekly. Results Growth rate, macro-minerals, micro-nutrients and nutritional values were similar to commercial culture medium in the media with inorganic fertilizer and macrophyte. The biological indexes of microalgae were not influenced by weekly differences during the study period. Although lipid contents, nitrogen and growth rate of A. gracilis were higher in inorganic fertilizer and macrophyte media; Mn, B, Cu, S, dry matter, ash content, cell volume, TOC and total length were similar among the media used. The same high cell density 25 x 105 cells.mL-1 was obtained in NPK and in CHU12 medium respectively after 11 and 14 days cultivation. Conclusion Culture media inorganic fertilizer (NPK) and macrophyte (Eichhornia crassipes), were adequate and may replace the commercial medium CHU12 for the cultivation of microalgae A. gracilis, resulting in high nutritional composition, high biomass, high nutrients and low-cost.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2017000100305&lng=en&tlng=enEichhornia crassipesNPK (20-5-20)composição bioquimícameio de cultura
spellingShingle Lúcia Helena Sipaúba-Tavares
Alexandra Maria Donadon Lusser Segali
Flavia Almeida Berchielli-Morais
Bruno Scardoeli-Truzzi
Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte
Acta Limnologica Brasiliensia
Eichhornia crassipes
NPK (20-5-20)
composição bioquimíca
meio de cultura
title Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte
title_full Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte
title_fullStr Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte
title_full_unstemmed Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte
title_short Development of low-cost culture media for Ankistrodesmus gracilis based on inorganic fertilizer and macrophyte
title_sort development of low cost culture media for ankistrodesmus gracilis based on inorganic fertilizer and macrophyte
topic Eichhornia crassipes
NPK (20-5-20)
composição bioquimíca
meio de cultura
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2179-975X2017000100305&lng=en&tlng=en
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AT flaviaalmeidaberchiellimorais developmentoflowcostculturemediaforankistrodesmusgracilisbasedoninorganicfertilizerandmacrophyte
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