Common and unique testosterone and 17 beta-estradiol degradation mechanisms in Comamonas testosteroni JLU460ET by transcriptome analysis
Strain C. testosteroni JLU460ET was isolated for testosterone and 17 beta-estradiol degradation by our group. In this study, strain C. testosteroni JLU460ET was induced by testosterone and 17 beta-estradiol and then subjected to transcriptome analysis. There were 2,047 upregulated genes after 3 h of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-10-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1238855/full |
| _version_ | 1797648882591596544 |
|---|---|
| author | Ze Wang Mingming Chen Na Liu Yongkang Zhao Jintao Ru Chuanyu Qin Tingdi Zhang |
| author_facet | Ze Wang Mingming Chen Na Liu Yongkang Zhao Jintao Ru Chuanyu Qin Tingdi Zhang |
| author_sort | Ze Wang |
| collection | DOAJ |
| description | Strain C. testosteroni JLU460ET was isolated for testosterone and 17 beta-estradiol degradation by our group. In this study, strain C. testosteroni JLU460ET was induced by testosterone and 17 beta-estradiol and then subjected to transcriptome analysis. There were 2,047 upregulated genes after 3 h of testosterone induction, 2,040 upregulated genes after 13 h of testosterone induction, 2,078 upregulated genes after 3 h of 17 beta-estradiol induction, and 2,095 upregulated genes after 13 h of 17 beta-estradiol induction. Significantly upregulated genes were mainly involved in steroid and aromatic compound degradation. A 100 kb steroid-degrading gene cluster was found by transcriptome analysis, which included 92 annotated genes and 58 novel genes. Among them, MucB/RseB and Fiu are secretory proteins for sensing substrates in the environment. MFS-1 and TonB are transporters of testosterone and 17 beta-estradiol. Ring-cleavage enzymes and beta-oxidation enzymes are important for degradation. The genes upregulated by both substrates were almost the same, but the degree of induction by testosterone was higher than that by 17 beta-estradiol. Nine upregulated genes were selected for verification by quantitative real-time polymerase chain reaction (qRT–PCR). The qRT-PCR results were consistent with the transcriptome sequencing results. In this study, the common and unique metabolic mechanisms of testosterone and 17 beta-estradiol were compared by transcriptome analysis in C. testosteroni JLU460ET for the first time. |
| first_indexed | 2024-03-11T15:38:22Z |
| format | Article |
| id | doaj.art-adf543676f834a5885224ca59a0aaeb7 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-11T15:38:22Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-adf543676f834a5885224ca59a0aaeb72023-10-26T13:47:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-10-011410.3389/fmicb.2023.12388551238855Common and unique testosterone and 17 beta-estradiol degradation mechanisms in Comamonas testosteroni JLU460ET by transcriptome analysisZe Wang0Mingming Chen1Na Liu2Yongkang Zhao3Jintao Ru4Chuanyu Qin5Tingdi Zhang6Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun, Jilin, ChinaKey Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun, Jilin, ChinaDepartment of Ecology, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong, ChinaKey Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun, Jilin, ChinaKey Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun, Jilin, ChinaKey Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun, Jilin, ChinaKey Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun, Jilin, ChinaStrain C. testosteroni JLU460ET was isolated for testosterone and 17 beta-estradiol degradation by our group. In this study, strain C. testosteroni JLU460ET was induced by testosterone and 17 beta-estradiol and then subjected to transcriptome analysis. There were 2,047 upregulated genes after 3 h of testosterone induction, 2,040 upregulated genes after 13 h of testosterone induction, 2,078 upregulated genes after 3 h of 17 beta-estradiol induction, and 2,095 upregulated genes after 13 h of 17 beta-estradiol induction. Significantly upregulated genes were mainly involved in steroid and aromatic compound degradation. A 100 kb steroid-degrading gene cluster was found by transcriptome analysis, which included 92 annotated genes and 58 novel genes. Among them, MucB/RseB and Fiu are secretory proteins for sensing substrates in the environment. MFS-1 and TonB are transporters of testosterone and 17 beta-estradiol. Ring-cleavage enzymes and beta-oxidation enzymes are important for degradation. The genes upregulated by both substrates were almost the same, but the degree of induction by testosterone was higher than that by 17 beta-estradiol. Nine upregulated genes were selected for verification by quantitative real-time polymerase chain reaction (qRT–PCR). The qRT-PCR results were consistent with the transcriptome sequencing results. In this study, the common and unique metabolic mechanisms of testosterone and 17 beta-estradiol were compared by transcriptome analysis in C. testosteroni JLU460ET for the first time.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1238855/fullC. testosteroni JLU460ETtestosterone17 beta-estradioltranscriptomedegradation |
| spellingShingle | Ze Wang Mingming Chen Na Liu Yongkang Zhao Jintao Ru Chuanyu Qin Tingdi Zhang Common and unique testosterone and 17 beta-estradiol degradation mechanisms in Comamonas testosteroni JLU460ET by transcriptome analysis Frontiers in Microbiology C. testosteroni JLU460ET testosterone 17 beta-estradiol transcriptome degradation |
| title | Common and unique testosterone and 17 beta-estradiol degradation mechanisms in Comamonas testosteroni JLU460ET by transcriptome analysis |
| title_full | Common and unique testosterone and 17 beta-estradiol degradation mechanisms in Comamonas testosteroni JLU460ET by transcriptome analysis |
| title_fullStr | Common and unique testosterone and 17 beta-estradiol degradation mechanisms in Comamonas testosteroni JLU460ET by transcriptome analysis |
| title_full_unstemmed | Common and unique testosterone and 17 beta-estradiol degradation mechanisms in Comamonas testosteroni JLU460ET by transcriptome analysis |
| title_short | Common and unique testosterone and 17 beta-estradiol degradation mechanisms in Comamonas testosteroni JLU460ET by transcriptome analysis |
| title_sort | common and unique testosterone and 17 beta estradiol degradation mechanisms in comamonas testosteroni jlu460et by transcriptome analysis |
| topic | C. testosteroni JLU460ET testosterone 17 beta-estradiol transcriptome degradation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1238855/full |
| work_keys_str_mv | AT zewang commonanduniquetestosteroneand17betaestradioldegradationmechanismsincomamonastestosteronijlu460etbytranscriptomeanalysis AT mingmingchen commonanduniquetestosteroneand17betaestradioldegradationmechanismsincomamonastestosteronijlu460etbytranscriptomeanalysis AT naliu commonanduniquetestosteroneand17betaestradioldegradationmechanismsincomamonastestosteronijlu460etbytranscriptomeanalysis AT yongkangzhao commonanduniquetestosteroneand17betaestradioldegradationmechanismsincomamonastestosteronijlu460etbytranscriptomeanalysis AT jintaoru commonanduniquetestosteroneand17betaestradioldegradationmechanismsincomamonastestosteronijlu460etbytranscriptomeanalysis AT chuanyuqin commonanduniquetestosteroneand17betaestradioldegradationmechanismsincomamonastestosteronijlu460etbytranscriptomeanalysis AT tingdizhang commonanduniquetestosteroneand17betaestradioldegradationmechanismsincomamonastestosteronijlu460etbytranscriptomeanalysis |