The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based Protein Phosphatases, Maintains Cellular Homeostasis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>
ABSTRACT Trypanosoma brucei, the infectious agent of a deadly disease known as African trypanosomiasis, undergoes various stresses during its digenetic life cycle. We previously showed that downregulation of T. brucei mitochondrial inner membrane protein translocase 50 (TbTim50), an aspartate-based...
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2019-08-01
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| Series: | mSphere |
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| Online Access: | https://journals.asm.org/doi/10.1128/mSphere.00353-19 |
| _version_ | 1818856358718472192 |
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| author | Anuj Tripathi Ujjal K. Singha Victor Paromov Salisha Hill Siddharth Pratap Kristie Rose Minu Chaudhuri |
| author_facet | Anuj Tripathi Ujjal K. Singha Victor Paromov Salisha Hill Siddharth Pratap Kristie Rose Minu Chaudhuri |
| author_sort | Anuj Tripathi |
| collection | DOAJ |
| description | ABSTRACT Trypanosoma brucei, the infectious agent of a deadly disease known as African trypanosomiasis, undergoes various stresses during its digenetic life cycle. We previously showed that downregulation of T. brucei mitochondrial inner membrane protein translocase 50 (TbTim50), an aspartate-based protein phosphatase and a component of the translocase of the mitochondrial inner membrane (TIM), increased the tolerance of procyclic cells to oxidative stress. Using comparative proteomics analysis and further validating the proteomics results by immunoblotting, here we discovered that TbTim50 downregulation caused an approximately 5-fold increase in the levels of PIP39, which is also an aspartate-based protein phosphatase and is primarily localized in glycosomes. A moderate upregulation of a number of glycosomal enzymes was also noticed due to TbTim50 knockdown. We found that the rate of mitochondrial ATP production by oxidative phosphorylation decreased and that substrate-level phosphorylation increased due to depletion of TbTim50. These results were correlated with relative increases in the levels of trypanosome alternative oxidase and hexokinase and a reduced-growth phenotype in low-glucose medium. The levels and activity of the mitochondrial superoxide dismutase and glutaredoxin levels were increased due to TbTim50 knockdown. Furthermore, we show that TbTim50 downregulation increased the cellular AMP/ATP ratio, and as a consequence, phosphorylation of AMP-activated protein kinase (AMPK) was increased. Knocking down both TbTim50 and TbPIP39 reduced PIP39 levels as well as AMPK phosphorylation and reduced T. brucei tolerance to oxidative stress. These results suggest that TbTim50 and PIP39, two protein phosphatases in mitochondria and glycosomes, respectively, cross talk via the AMPK pathway to maintain cellular homeostasis in the procyclic form of T. brucei. IMPORTANCE Trypanosoma brucei, the infectious agent of African trypanosomiasis, must adapt to strikingly different host environments during its digenetic life cycle. Developmental regulation of mitochondrial activities is an essential part of these processes. We have shown previously that mitochondrial inner membrane protein translocase 50 in T. brucei (TbTim50) possesses a dually specific phosphatase activity and plays a role in the cellular stress response pathway. Using proteomics analysis, here we have elucidated a novel connection between TbTim50 and a protein phosphatase of the same family, PIP39, which is also a differentiation-related protein localized in glycosomes. We found that these two protein phosphatases cross talk via the AMPK pathway and modulate cellular metabolic activities under stress. Together, our results indicate the importance of a TbTim50 and PIP39 cascade for communication between mitochondria and other cellular parts in regulation of cell homeostasis in T. brucei. |
| first_indexed | 2024-12-19T08:23:14Z |
| format | Article |
| id | doaj.art-adf9685fe8934cc8ba9b3c2b6c2aed4b |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-12-19T08:23:14Z |
| publishDate | 2019-08-01 |
| publisher | American Society for Microbiology |
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| series | mSphere |
| spelling | doaj.art-adf9685fe8934cc8ba9b3c2b6c2aed4b2022-12-21T20:29:22ZengAmerican Society for MicrobiologymSphere2379-50422019-08-014410.1128/mSphere.00353-19The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based Protein Phosphatases, Maintains Cellular Homeostasis in <named-content content-type="genus-species">Trypanosoma brucei</named-content>Anuj Tripathi0Ujjal K. Singha1Victor Paromov2Salisha Hill3Siddharth Pratap4Kristie Rose5Minu Chaudhuri6Department of Microbiology, Immunology, and Physiology, Meharry Medical College, Nashville, Tennessee, USADepartment of Microbiology, Immunology, and Physiology, Meharry Medical College, Nashville, Tennessee, USADepartment of Microbiology, Immunology, and Physiology, Meharry Medical College, Nashville, Tennessee, USADepartment of Biochemistry, Vanderbilt University, Nashville, Tennessee, USADepartment of Microbiology, Immunology, and Physiology, Meharry Medical College, Nashville, Tennessee, USADepartment of Biochemistry, Vanderbilt University, Nashville, Tennessee, USADepartment of Microbiology, Immunology, and Physiology, Meharry Medical College, Nashville, Tennessee, USAABSTRACT Trypanosoma brucei, the infectious agent of a deadly disease known as African trypanosomiasis, undergoes various stresses during its digenetic life cycle. We previously showed that downregulation of T. brucei mitochondrial inner membrane protein translocase 50 (TbTim50), an aspartate-based protein phosphatase and a component of the translocase of the mitochondrial inner membrane (TIM), increased the tolerance of procyclic cells to oxidative stress. Using comparative proteomics analysis and further validating the proteomics results by immunoblotting, here we discovered that TbTim50 downregulation caused an approximately 5-fold increase in the levels of PIP39, which is also an aspartate-based protein phosphatase and is primarily localized in glycosomes. A moderate upregulation of a number of glycosomal enzymes was also noticed due to TbTim50 knockdown. We found that the rate of mitochondrial ATP production by oxidative phosphorylation decreased and that substrate-level phosphorylation increased due to depletion of TbTim50. These results were correlated with relative increases in the levels of trypanosome alternative oxidase and hexokinase and a reduced-growth phenotype in low-glucose medium. The levels and activity of the mitochondrial superoxide dismutase and glutaredoxin levels were increased due to TbTim50 knockdown. Furthermore, we show that TbTim50 downregulation increased the cellular AMP/ATP ratio, and as a consequence, phosphorylation of AMP-activated protein kinase (AMPK) was increased. Knocking down both TbTim50 and TbPIP39 reduced PIP39 levels as well as AMPK phosphorylation and reduced T. brucei tolerance to oxidative stress. These results suggest that TbTim50 and PIP39, two protein phosphatases in mitochondria and glycosomes, respectively, cross talk via the AMPK pathway to maintain cellular homeostasis in the procyclic form of T. brucei. IMPORTANCE Trypanosoma brucei, the infectious agent of African trypanosomiasis, must adapt to strikingly different host environments during its digenetic life cycle. Developmental regulation of mitochondrial activities is an essential part of these processes. We have shown previously that mitochondrial inner membrane protein translocase 50 in T. brucei (TbTim50) possesses a dually specific phosphatase activity and plays a role in the cellular stress response pathway. Using proteomics analysis, here we have elucidated a novel connection between TbTim50 and a protein phosphatase of the same family, PIP39, which is also a differentiation-related protein localized in glycosomes. We found that these two protein phosphatases cross talk via the AMPK pathway and modulate cellular metabolic activities under stress. Together, our results indicate the importance of a TbTim50 and PIP39 cascade for communication between mitochondria and other cellular parts in regulation of cell homeostasis in T. brucei.https://journals.asm.org/doi/10.1128/mSphere.00353-19AMPKPIP39Tim50Trypanosoma bruceistress tolerance |
| spellingShingle | Anuj Tripathi Ujjal K. Singha Victor Paromov Salisha Hill Siddharth Pratap Kristie Rose Minu Chaudhuri The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based Protein Phosphatases, Maintains Cellular Homeostasis in <named-content content-type="genus-species">Trypanosoma brucei</named-content> mSphere AMPK PIP39 Tim50 Trypanosoma brucei stress tolerance |
| title | The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based Protein Phosphatases, Maintains Cellular Homeostasis in <named-content content-type="genus-species">Trypanosoma brucei</named-content> |
| title_full | The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based Protein Phosphatases, Maintains Cellular Homeostasis in <named-content content-type="genus-species">Trypanosoma brucei</named-content> |
| title_fullStr | The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based Protein Phosphatases, Maintains Cellular Homeostasis in <named-content content-type="genus-species">Trypanosoma brucei</named-content> |
| title_full_unstemmed | The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based Protein Phosphatases, Maintains Cellular Homeostasis in <named-content content-type="genus-species">Trypanosoma brucei</named-content> |
| title_short | The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based Protein Phosphatases, Maintains Cellular Homeostasis in <named-content content-type="genus-species">Trypanosoma brucei</named-content> |
| title_sort | cross talk between tbtim50 and pip39 two aspartate based protein phosphatases maintains cellular homeostasis in named content content type genus species trypanosoma brucei named content |
| topic | AMPK PIP39 Tim50 Trypanosoma brucei stress tolerance |
| url | https://journals.asm.org/doi/10.1128/mSphere.00353-19 |
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