Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold Nanoparticles
Biosynthesis of gold nanoparticles (AuNPs) is emerging as a better alternative to traditional chemical-based techniques. During this study, extracts of different marine algae species Ulva rigida (green algae), Cystoseira myrica (brown Algae), and Gracilaria foliifera (red Algae) were utilized as red...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2022-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/anticancer-and-antimicrobial-activity-of-red-sea-seaweeds-extracts-mediated-gold-nanoparticles/ |
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| author | Rabaa Algotiml Ali Gab-alla Roshdi Seoudi Hussein H. Abulreesh Iqbal Ahmad Khaled Elbanna |
| author_facet | Rabaa Algotiml Ali Gab-alla Roshdi Seoudi Hussein H. Abulreesh Iqbal Ahmad Khaled Elbanna |
| author_sort | Rabaa Algotiml |
| collection | DOAJ |
| description | Biosynthesis of gold nanoparticles (AuNPs) is emerging as a better alternative to traditional chemical-based techniques. During this study, extracts of different marine algae species Ulva rigida (green algae), Cystoseira myrica (brown Algae), and Gracilaria foliifera (red Algae) were utilized as reducing and capping agents to synthesize AuNPs. AuNPs capped by U. rigida, C. myrica, and G. foliifera were confirmed by the appearance of surface plasmonic bands at 528, 540, and 543 nm, respectively. Transmission electron microscopy revealed mostly spherical shapes of AuNPs having a size of about 9 nm, 11 nm, and 13 nm for C. myrica, and G. foliifera extracts, respectively. Fourier transform-infrared spectroscopy (FTIR) illustrated the major chemical constituents of U. rigida, C. myrica, and G. foliifera. LC50 values of the biosynthesized AuNPs against Artemia salina nauplii were calculated at a range of concentrations (5-188 μg ml−1) after 16 to 24h. AuNPs concentration-dependent lethality was noted and U. rigida extracts-mediated AuNPs presented the lowest cytotoxicity. The biosynthesized AuNPs exhibited significant anticancer activity (86.83%) against MCF-7 cell lines (human breast adenocarcinoma cell lines) at 188 µg/ml concentration. G. foliifera demonstrated the highest anticancer value (92.13%) followed by C. myrica (89.82%), and U. rigida (86.83%), respectively. The AuNPs synthesized by different algal extracts showed variable antimicrobial activity against the tested pathogenic microorganisms. AuNPs of U. rigida extracts showed significant antimicrobial activity against dermatophytic fungi Trichosporon cataneum (30 mm) followed by Trichophyton mantigrophytes (25 mm). Furthermore, it also exhibited mild activity against Escherichia coli (17 mm), Cryptococcus neoformans (15 mm), Candida albicans (13 mm), and Staphylococcus aureus (11mm), respectively whereas no effects were observed against Bacillus cereus. To conclude, AuNPs can be effectively synthesized by marine algal species, and particularly U. rigida extracts could be effective reducing agents for the green AuNPs synthesis. These AuNPs could potentially serve as efficient alternative anticancer agents against human breast adenocarcinoma and anti-dermatophytes associated with skin infections. |
| first_indexed | 2024-12-21T10:54:46Z |
| format | Article |
| id | doaj.art-adfb4bddee0641eab2623345b30bd0b4 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-21T10:54:46Z |
| publishDate | 2022-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-adfb4bddee0641eab2623345b30bd0b42022-12-21T19:06:32ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2022-03-01161207225https://doi.org/10.22207/JPAM.16.1.11Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold NanoparticlesRabaa AlgotimlAli Gab-allaRoshdi SeoudiHussein H. Abulreeshhttps://orcid.org/0000-0002-3289-696XIqbal Ahmadhttps://orcid.org/0000-0001-8447-4497Khaled Elbannahttps://orcid.org/0000-0002-2011-9119Biosynthesis of gold nanoparticles (AuNPs) is emerging as a better alternative to traditional chemical-based techniques. During this study, extracts of different marine algae species Ulva rigida (green algae), Cystoseira myrica (brown Algae), and Gracilaria foliifera (red Algae) were utilized as reducing and capping agents to synthesize AuNPs. AuNPs capped by U. rigida, C. myrica, and G. foliifera were confirmed by the appearance of surface plasmonic bands at 528, 540, and 543 nm, respectively. Transmission electron microscopy revealed mostly spherical shapes of AuNPs having a size of about 9 nm, 11 nm, and 13 nm for C. myrica, and G. foliifera extracts, respectively. Fourier transform-infrared spectroscopy (FTIR) illustrated the major chemical constituents of U. rigida, C. myrica, and G. foliifera. LC50 values of the biosynthesized AuNPs against Artemia salina nauplii were calculated at a range of concentrations (5-188 μg ml−1) after 16 to 24h. AuNPs concentration-dependent lethality was noted and U. rigida extracts-mediated AuNPs presented the lowest cytotoxicity. The biosynthesized AuNPs exhibited significant anticancer activity (86.83%) against MCF-7 cell lines (human breast adenocarcinoma cell lines) at 188 µg/ml concentration. G. foliifera demonstrated the highest anticancer value (92.13%) followed by C. myrica (89.82%), and U. rigida (86.83%), respectively. The AuNPs synthesized by different algal extracts showed variable antimicrobial activity against the tested pathogenic microorganisms. AuNPs of U. rigida extracts showed significant antimicrobial activity against dermatophytic fungi Trichosporon cataneum (30 mm) followed by Trichophyton mantigrophytes (25 mm). Furthermore, it also exhibited mild activity against Escherichia coli (17 mm), Cryptococcus neoformans (15 mm), Candida albicans (13 mm), and Staphylococcus aureus (11mm), respectively whereas no effects were observed against Bacillus cereus. To conclude, AuNPs can be effectively synthesized by marine algal species, and particularly U. rigida extracts could be effective reducing agents for the green AuNPs synthesis. These AuNPs could potentially serve as efficient alternative anticancer agents against human breast adenocarcinoma and anti-dermatophytes associated with skin infections.https://microbiologyjournal.org/anticancer-and-antimicrobial-activity-of-red-sea-seaweeds-extracts-mediated-gold-nanoparticles/marine algaebiosynthesized gold nanoparticlescytotoxicityartemia salinaanticancerbreast cancer cell linesantibacterialantifungaldermatophytes |
| spellingShingle | Rabaa Algotiml Ali Gab-alla Roshdi Seoudi Hussein H. Abulreesh Iqbal Ahmad Khaled Elbanna Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold Nanoparticles Journal of Pure and Applied Microbiology marine algae biosynthesized gold nanoparticles cytotoxicity artemia salina anticancer breast cancer cell lines antibacterial antifungal dermatophytes |
| title | Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold Nanoparticles |
| title_full | Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold Nanoparticles |
| title_fullStr | Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold Nanoparticles |
| title_full_unstemmed | Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold Nanoparticles |
| title_short | Anticancer and Antimicrobial Activity of Red Sea Seaweeds Extracts-Mediated Gold Nanoparticles |
| title_sort | anticancer and antimicrobial activity of red sea seaweeds extracts mediated gold nanoparticles |
| topic | marine algae biosynthesized gold nanoparticles cytotoxicity artemia salina anticancer breast cancer cell lines antibacterial antifungal dermatophytes |
| url | https://microbiologyjournal.org/anticancer-and-antimicrobial-activity-of-red-sea-seaweeds-extracts-mediated-gold-nanoparticles/ |
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