Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018
ABSTRACT: Objectives: A retrospective study was undertaken to investigate the circulating dengue virus (DENV) serotypes and genotypes in India in 2018. Methods: In total, 4963 samples referred to virus research diagnostic laboratories (n=21), the Indian Council of Medical Research-National Institut...
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Elsevier
2021-10-01
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| Series: | International Journal of Infectious Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971221006780 |
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| author | K. Alagarasu J.A. Patil M.B. Kakade A.M. More B. Yogesh P. Newase S.M. Jadhav D. Parashar H. Kaur N. Gupta N. Vijay J. Narayan P.S. Shah Dr Ajanta Sharma Dr Amita Jain Dr Anudita Bhargava Dr B. Anukumar Dr Ashok M Dr Bharti Malhotra Dr B.J. Borkakoty Dr Debasis Biswas Dr SC Jaryal Dr Kanwardeep Singh Dhingra Dr K. Kaveri Dr P.K. Khatri Dr Manoj Kumar Dr K. Nagamani Dr Rahul Dhodapkar Dr Rupinder Bakshi Dr Paramjeet S. Gill Dr Sanghamitra Pati Dr Shanta Dutta Dr Shantala G Dr Usha Kalawat Dr Disha A. Patel Dr P. Vijayachari |
| author_facet | K. Alagarasu J.A. Patil M.B. Kakade A.M. More B. Yogesh P. Newase S.M. Jadhav D. Parashar H. Kaur N. Gupta N. Vijay J. Narayan P.S. Shah Dr Ajanta Sharma Dr Amita Jain Dr Anudita Bhargava Dr B. Anukumar Dr Ashok M Dr Bharti Malhotra Dr B.J. Borkakoty Dr Debasis Biswas Dr SC Jaryal Dr Kanwardeep Singh Dhingra Dr K. Kaveri Dr P.K. Khatri Dr Manoj Kumar Dr K. Nagamani Dr Rahul Dhodapkar Dr Rupinder Bakshi Dr Paramjeet S. Gill Dr Sanghamitra Pati Dr Shanta Dutta Dr Shantala G Dr Usha Kalawat Dr Disha A. Patel Dr P. Vijayachari |
| author_sort | K. Alagarasu |
| collection | DOAJ |
| description | ABSTRACT: Objectives: A retrospective study was undertaken to investigate the circulating dengue virus (DENV) serotypes and genotypes in India in 2018. Methods: In total, 4963 samples referred to virus research diagnostic laboratories (n=21), the Indian Council of Medical Research-National Institute of Virology (ICMR-NIV) and ICMR-NIV field units (n=2) for diagnosis of dengue in 2018 were tested using a real-time reverse transcription polymerase chain reaction assay for the presence of DENV serotypes. Representative samples were sequenced for the envelope (E) gene. Results: Regional diversity was observed with regard to the dominant circulating serotypes. DENV-2 was found to be the most common serotype in many states. Thrombocytopenia, petechiae and malaise were associated with DENV-2 infection. Phylogenetic analyses of DENV E gene sequences revealed the circulation of genotypes I and V of DENV-1, two lineages of DENV-2 genotype IV, DENV-3 genotype III and DENV-4 genotype I. Conclusions: This study found regional differences in the prevalence of circulating DENV serotypes in India, and provides baseline data for continuous molecular surveillance. Molecular surveillance may have implications for predicting large-scale outbreaks of dengue if regional shifts in the predominantly circulating serotypes and genotypes are detected during the early phase of the dengue season. |
| first_indexed | 2024-12-17T12:24:21Z |
| format | Article |
| id | doaj.art-adfbe8aa704b4222a86b334785bc277e |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-17T12:24:21Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-adfbe8aa704b4222a86b334785bc277e2022-12-21T21:48:50ZengElsevierInternational Journal of Infectious Diseases1201-97122021-10-01111242252Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018K. Alagarasu0J.A. Patil1M.B. Kakade2A.M. More3B. Yogesh4P. Newase5S.M. Jadhav6D. Parashar7H. Kaur8N. Gupta9N. Vijay10J. Narayan11P.S. Shah12Dr Ajanta Sharma13Dr Amita Jain14Dr Anudita Bhargava15Dr B. Anukumar16Dr Ashok M17Dr Bharti Malhotra18Dr B.J. Borkakoty19Dr Debasis Biswas20Dr SC Jaryal21Dr Kanwardeep Singh Dhingra22Dr K. Kaveri23Dr P.K. Khatri24Dr Manoj Kumar25Dr K. Nagamani26Dr Rahul Dhodapkar27Dr Rupinder Bakshi28Dr Paramjeet S. Gill29Dr Sanghamitra Pati30Dr Shanta Dutta31Dr Shantala G32Dr Usha Kalawat33Dr Disha A. Patel34Dr P. Vijayachari35Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, India; Corresponding author. Address: Dengue/Chikungunya Group, ICMR-National Institute of Virology, Pune 411001, Maharashtra, India.Dengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, IndiaDengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, IndiaDengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, IndiaDengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, IndiaDengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, IndiaDengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, IndiaDengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, IndiaVirology Unit, Indian Council of Medical Research, New Delhi, IndiaVirology Unit, Indian Council of Medical Research, New Delhi, IndiaVirology Unit, Indian Council of Medical Research, New Delhi, IndiaVirology Unit, Indian Council of Medical Research, New Delhi, IndiaDengue and Chikungunya Group, ICMR-National Institute of Virology, Pune, Maharashtra, IndiaGauhati Medical College, GauhatiKing George Medical University, LucknowAll India Institute of Medical Sciences, RaipurICMR-NIV Field Unit, AlapuzhaICMR-NIV Filed Unit, BengaluruSMS Medical College, JaipurICMR-Regional Research Medical Centre, DibrugarhAll India Institute of Medical Sciences, BhopalDr. Rajendra Prasad Government Medical College, TandaGovernment Medical College, AmritsarKing Institute of Preventive Medicine & Research, Guindy, ChennaiSN Medical College, JodhpurRajendra Institute of Medical Sciences, RanchiGandhi Medical College, SecunderabadJawaharlal Nehru Institute of Postgraduate Medical Education and Research, PuducherryGovernment Medical College, PatialaPost Graduate Institute of Medical Sciences, RohtakRegional Medical Research Centre, BhubaneswarICMR-National Institute of Cholera & Enteric Diseases, KolkataBangalore Medical College & Research Institute, BengaluruSri Venkateswara Institute of Medical Sciences, TirupatiB.J. Medical College, AhmedabadICMR-Regional Research Medical Centre, Port BlairABSTRACT: Objectives: A retrospective study was undertaken to investigate the circulating dengue virus (DENV) serotypes and genotypes in India in 2018. Methods: In total, 4963 samples referred to virus research diagnostic laboratories (n=21), the Indian Council of Medical Research-National Institute of Virology (ICMR-NIV) and ICMR-NIV field units (n=2) for diagnosis of dengue in 2018 were tested using a real-time reverse transcription polymerase chain reaction assay for the presence of DENV serotypes. Representative samples were sequenced for the envelope (E) gene. Results: Regional diversity was observed with regard to the dominant circulating serotypes. DENV-2 was found to be the most common serotype in many states. Thrombocytopenia, petechiae and malaise were associated with DENV-2 infection. Phylogenetic analyses of DENV E gene sequences revealed the circulation of genotypes I and V of DENV-1, two lineages of DENV-2 genotype IV, DENV-3 genotype III and DENV-4 genotype I. Conclusions: This study found regional differences in the prevalence of circulating DENV serotypes in India, and provides baseline data for continuous molecular surveillance. Molecular surveillance may have implications for predicting large-scale outbreaks of dengue if regional shifts in the predominantly circulating serotypes and genotypes are detected during the early phase of the dengue season.http://www.sciencedirect.com/science/article/pii/S1201971221006780Dengue virus serotypesGenotypesMolecular surveillanceVirus Research Diagnostic Laboratory (VRDL) Network |
| spellingShingle | K. Alagarasu J.A. Patil M.B. Kakade A.M. More B. Yogesh P. Newase S.M. Jadhav D. Parashar H. Kaur N. Gupta N. Vijay J. Narayan P.S. Shah Dr Ajanta Sharma Dr Amita Jain Dr Anudita Bhargava Dr B. Anukumar Dr Ashok M Dr Bharti Malhotra Dr B.J. Borkakoty Dr Debasis Biswas Dr SC Jaryal Dr Kanwardeep Singh Dhingra Dr K. Kaveri Dr P.K. Khatri Dr Manoj Kumar Dr K. Nagamani Dr Rahul Dhodapkar Dr Rupinder Bakshi Dr Paramjeet S. Gill Dr Sanghamitra Pati Dr Shanta Dutta Dr Shantala G Dr Usha Kalawat Dr Disha A. Patel Dr P. Vijayachari Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018 International Journal of Infectious Diseases Dengue virus serotypes Genotypes Molecular surveillance Virus Research Diagnostic Laboratory (VRDL) Network |
| title | Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018 |
| title_full | Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018 |
| title_fullStr | Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018 |
| title_full_unstemmed | Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018 |
| title_short | Serotype and genotype diversity of dengue viruses circulating in India: a multi-centre retrospective study involving the Virus Research Diagnostic Laboratory Network in 2018 |
| title_sort | serotype and genotype diversity of dengue viruses circulating in india a multi centre retrospective study involving the virus research diagnostic laboratory network in 2018 |
| topic | Dengue virus serotypes Genotypes Molecular surveillance Virus Research Diagnostic Laboratory (VRDL) Network |
| url | http://www.sciencedirect.com/science/article/pii/S1201971221006780 |
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