| Summary: | Broomcorn millet Huangjiu brewing is usually divided into primary fermentation and post-fermentation. Microbial succession is the major factor influencing the development of the typical Huangjiu flavor. Here, we report the changes in flavor substances and microbial community during the primary fermentation of broomcorn millet Huangjiu. Results indicated that a total of 161 volatile flavor compounds were measured during primary fermentation, and estragole was detected for the first time in broomcorn millet Huangjiu. A total of 82 bacteria genera were identified. <i>Pediococcus</i>, <i>Pantoea</i>, and <i>Weissella</i> were the dominant genera. <i>Saccharomyces</i> and <i>Rhizopus</i> were dominant among the 30 fungal genera. Correlation analysis showed that 102 microorganisms were involved in major flavor substance production during primary fermentation, <i>Lactobacillus</i>, <i>Photobacterium</i>, <i>Hyphodontia</i>, <i>Aquicella</i>, <i>Erysipelothrix</i>, <i>Idiomarina</i>, <i>Paraphaeosphaeria</i>, and <i>Sulfuritalea</i> were most associated with flavoring substances. Four bacteria, <i>Lactobacillus</i> (R1), <i>Photobacterium</i> (R2), <i>Idiomarina</i> (R3), and <i>Pediococcus</i> (R4), were isolated and identified from wheat Qu, which were added to wine Qu to prepare four kinds of fortified Qu (QR1, QR2, QR3, QR4). QR1 and QR2 fermentation can enhance the quality of Huangjiu. This work reveals the correlation between microorganisms and volatile flavor compounds and is beneficial for regulating the micro-ecosystem and flavor of the broomcorn millet Huangjiu.
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