Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice
E3 ubiquitin ligases are involved in many processes, regulating the response to biotic and abiotic stresses. In this study, 11 E3 ubiquitin ligase genes from Arabidopsis, which were hypothesized to function in response to biotic or abiotic stresses were selected, and the homologous genes in rice wer...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.840360/full |
| _version_ | 1818914759977730048 |
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| author | Huijuan Zhang Dewei Zheng Fengming Song Ming Jiang |
| author_facet | Huijuan Zhang Dewei Zheng Fengming Song Ming Jiang |
| author_sort | Huijuan Zhang |
| collection | DOAJ |
| description | E3 ubiquitin ligases are involved in many processes, regulating the response to biotic and abiotic stresses. In this study, 11 E3 ubiquitin ligase genes from Arabidopsis, which were hypothesized to function in response to biotic or abiotic stresses were selected, and the homologous genes in rice were found. Their functions were analyzed in rice. These 11 E3 ubiquitin ligase genes showed different patterns of expression under different treatments. The BMV:OsPUB39-infiltrated seedlings showed decreased resistance to Magnaporthe grisea (M. grisea) when compared with BMV:00-infiltrated seedlings, whereas the BMV:OsPUB34- and BMV:OsPUB33-infiltrated seedlings showed increased resistance. The involvement of these genes in the resistance against M. grisea may be attributed to the regulation of the accumulation of reactive oxygen species (ROS) and expression levels of defense-related genes. Seedlings infiltrated by BMV:OsATL69 showed decreased tolerance to drought stress, whereas BMV:OsPUB33-infiltraed seedlings showed increased tolerance, possibly through the regulation of proline content, sugar content, and expression of drought-responsive genes. BMV:OsATL32-infiltrated seedlings showed decreased tolerance to cold stress by regulating malondialdehyde (MDA) content and the expression of cold-responsive genes. |
| first_indexed | 2024-12-19T23:51:30Z |
| format | Article |
| id | doaj.art-adfd61f5e97d41d2b83ccadeb5fffa73 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T23:51:30Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-adfd61f5e97d41d2b83ccadeb5fffa732022-12-21T20:01:07ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-03-011310.3389/fpls.2022.840360840360Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in RiceHuijuan Zhang0Dewei Zheng1Fengming Song2Ming Jiang3College of Life Science, Taizhou University, Taizhou, ChinaCollege of Life Science, Taizhou University, Taizhou, ChinaNational Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, ChinaCollege of Life Science, Taizhou University, Taizhou, ChinaE3 ubiquitin ligases are involved in many processes, regulating the response to biotic and abiotic stresses. In this study, 11 E3 ubiquitin ligase genes from Arabidopsis, which were hypothesized to function in response to biotic or abiotic stresses were selected, and the homologous genes in rice were found. Their functions were analyzed in rice. These 11 E3 ubiquitin ligase genes showed different patterns of expression under different treatments. The BMV:OsPUB39-infiltrated seedlings showed decreased resistance to Magnaporthe grisea (M. grisea) when compared with BMV:00-infiltrated seedlings, whereas the BMV:OsPUB34- and BMV:OsPUB33-infiltrated seedlings showed increased resistance. The involvement of these genes in the resistance against M. grisea may be attributed to the regulation of the accumulation of reactive oxygen species (ROS) and expression levels of defense-related genes. Seedlings infiltrated by BMV:OsATL69 showed decreased tolerance to drought stress, whereas BMV:OsPUB33-infiltraed seedlings showed increased tolerance, possibly through the regulation of proline content, sugar content, and expression of drought-responsive genes. BMV:OsATL32-infiltrated seedlings showed decreased tolerance to cold stress by regulating malondialdehyde (MDA) content and the expression of cold-responsive genes.https://www.frontiersin.org/articles/10.3389/fpls.2022.840360/fullriceE3 ubiquitin ligasesbiotic stressabiotic stressROSexpression patterns |
| spellingShingle | Huijuan Zhang Dewei Zheng Fengming Song Ming Jiang Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice Frontiers in Plant Science rice E3 ubiquitin ligases biotic stress abiotic stress ROS expression patterns |
| title | Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice |
| title_full | Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice |
| title_fullStr | Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice |
| title_full_unstemmed | Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice |
| title_short | Expression Patterns and Functional Analysis of 11 E3 Ubiquitin Ligase Genes in Rice |
| title_sort | expression patterns and functional analysis of 11 e3 ubiquitin ligase genes in rice |
| topic | rice E3 ubiquitin ligases biotic stress abiotic stress ROS expression patterns |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.840360/full |
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