Proteome-metabolome profiling of wax gland complex reveals functional changes in honeybee, Apis mellifera L.
Summary: Wax gland complex (WGC) serves as the primary generator of beeswax; however, the dynamic biological function in wax secretion remains unclear. To elucidate the developmental mechanism of WGC, we conducted a comprehensive analysis to reveal the variations in proteins and metabolites among th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004224005005 |
| _version_ | 1797271343687794688 |
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| author | Rumeng Xu Beibei Ma Yiying Yang Xuancheng Dong Jianke Li Xiang Xu Yu Fang |
| author_facet | Rumeng Xu Beibei Ma Yiying Yang Xuancheng Dong Jianke Li Xiang Xu Yu Fang |
| author_sort | Rumeng Xu |
| collection | DOAJ |
| description | Summary: Wax gland complex (WGC) serves as the primary generator of beeswax; however, the dynamic biological function in wax secretion remains unclear. To elucidate the developmental mechanism of WGC, we conducted a comprehensive analysis to reveal the variations in proteins and metabolites among the newly emerged bee (NEB), wax-secreting bee (WSB), and overaged bee (OAB). We identified 3,295 proteins and 159 metabolites in WGC. Specifically, NEB elevated the expression of ribosomal proteins for preparing the glandular organ. While WSB promoted the size of epidermal cells and oenocytes, the enrichment of fatty acids and energy metabolism in WSB suggested a strong ability in wax synthesis. In OAB, disorganized wax tubules, and up-regulated cysteine proteases reflected the gland degeneration. These findings highlight the dynamic changes in the level of molecule and morphological structure in WGC, offering valuable insights into the development and mechanism of wax secretion in honeybees and other wax insects. |
| first_indexed | 2024-03-07T14:00:20Z |
| format | Article |
| id | doaj.art-ae009e415c2342558cbdb6d2c1778e55 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-07T14:00:20Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-ae009e415c2342558cbdb6d2c1778e552024-03-07T05:29:00ZengElsevieriScience2589-00422024-03-01273109279Proteome-metabolome profiling of wax gland complex reveals functional changes in honeybee, Apis mellifera L.Rumeng Xu0Beibei Ma1Yiying Yang2Xuancheng Dong3Jianke Li4Xiang Xu5Yu Fang6State Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Corresponding authorState Key Laboratory of Resource Insects, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Corresponding authorSummary: Wax gland complex (WGC) serves as the primary generator of beeswax; however, the dynamic biological function in wax secretion remains unclear. To elucidate the developmental mechanism of WGC, we conducted a comprehensive analysis to reveal the variations in proteins and metabolites among the newly emerged bee (NEB), wax-secreting bee (WSB), and overaged bee (OAB). We identified 3,295 proteins and 159 metabolites in WGC. Specifically, NEB elevated the expression of ribosomal proteins for preparing the glandular organ. While WSB promoted the size of epidermal cells and oenocytes, the enrichment of fatty acids and energy metabolism in WSB suggested a strong ability in wax synthesis. In OAB, disorganized wax tubules, and up-regulated cysteine proteases reflected the gland degeneration. These findings highlight the dynamic changes in the level of molecule and morphological structure in WGC, offering valuable insights into the development and mechanism of wax secretion in honeybees and other wax insects.http://www.sciencedirect.com/science/article/pii/S2589004224005005EntomologyEvolutionary biologyMetabolomicsProteomicsZoology |
| spellingShingle | Rumeng Xu Beibei Ma Yiying Yang Xuancheng Dong Jianke Li Xiang Xu Yu Fang Proteome-metabolome profiling of wax gland complex reveals functional changes in honeybee, Apis mellifera L. iScience Entomology Evolutionary biology Metabolomics Proteomics Zoology |
| title | Proteome-metabolome profiling of wax gland complex reveals functional changes in honeybee, Apis mellifera L. |
| title_full | Proteome-metabolome profiling of wax gland complex reveals functional changes in honeybee, Apis mellifera L. |
| title_fullStr | Proteome-metabolome profiling of wax gland complex reveals functional changes in honeybee, Apis mellifera L. |
| title_full_unstemmed | Proteome-metabolome profiling of wax gland complex reveals functional changes in honeybee, Apis mellifera L. |
| title_short | Proteome-metabolome profiling of wax gland complex reveals functional changes in honeybee, Apis mellifera L. |
| title_sort | proteome metabolome profiling of wax gland complex reveals functional changes in honeybee apis mellifera l |
| topic | Entomology Evolutionary biology Metabolomics Proteomics Zoology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004224005005 |
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