Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic Escherichia coli
Background and Objectives: Infection with Infectious bronchitis virus (IBV) and avian pathogenic Escherichia coli (APEC) is an important respiratory infection worldwide. Apoptosis is a physiological process of cell death that occurs as part of normal development and responds to a variety of physiolo...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2021-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/2847 |
| _version_ | 1819053835115560960 |
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| author | Shabnam Hashemi Seyed Masoud Hosseini Arash Ghalyanchilangeroudi Nariman Sheikhi |
| author_facet | Shabnam Hashemi Seyed Masoud Hosseini Arash Ghalyanchilangeroudi Nariman Sheikhi |
| author_sort | Shabnam Hashemi |
| collection | DOAJ |
| description | Background and Objectives: Infection with Infectious bronchitis virus (IBV) and avian pathogenic Escherichia coli (APEC) is an important respiratory infection worldwide. Apoptosis is a physiological process of cell death that occurs as part of normal development and responds to a variety of physiological and pathophysiological stimuli. The identification of molecular mechanisms of action or inaction of key apoptotic proteins is important. This study aimed to investigate apoptotic related genes in the trachea tissue of infected (IBV variant 2, and APEC serotype O78: K80) SPF chickens group compared to the control group.
Materials and Methods: Forty SPF chickens was divided into 2 groups. Differential transcriptional profile in the infected SPF chickens trachea tissue was compared to those of control group in the early stage of infection by Illumina RNA-seq technique paired-end and strand-specific sequencing. Differentially expressed genes (DEGs) of transcriptome profiling of the trachea from the infected group were identified. Gene ontology category, KEGG pathway, and STRING analysis were analyzed to identify relationships among differentially expressed genes.
Results: Twenty-eight apoptotic genes were identified. They consisted of six pathways related to cell death: the extrinsic pathway, intrinsic pathway, endoplasmic reticulum stress pathway, MAPK signaling pathway, and cell death by NFkB and activates mTOR pathway and some regulator and apoptosis inhibitors.
Conclusion: All of the apoptotic genes in our study were up-regulated. Among these genes, the more fold change value was for TRADD and BCL2A1 genes, and the less fold change value was for MAP3K14, NFKB1, PIK3CB, and ITPR2 genes. |
| first_indexed | 2024-12-21T12:42:02Z |
| format | Article |
| id | doaj.art-ae04ed31b89143de8dcf8f25a73e6eca |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-21T12:42:02Z |
| publishDate | 2021-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-ae04ed31b89143de8dcf8f25a73e6eca2022-12-21T19:03:45ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472021-02-0113117222847Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic Escherichia coliShabnam Hashemi0Seyed Masoud Hosseini1Arash Ghalyanchilangeroudi2Nariman Sheikhi3Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranDepartment of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranDepartment of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Clinical Sciences, College of Veterinary, Tehran Sciences and Research Branch, Islamic Azad University, Tehran, IranBackground and Objectives: Infection with Infectious bronchitis virus (IBV) and avian pathogenic Escherichia coli (APEC) is an important respiratory infection worldwide. Apoptosis is a physiological process of cell death that occurs as part of normal development and responds to a variety of physiological and pathophysiological stimuli. The identification of molecular mechanisms of action or inaction of key apoptotic proteins is important. This study aimed to investigate apoptotic related genes in the trachea tissue of infected (IBV variant 2, and APEC serotype O78: K80) SPF chickens group compared to the control group. Materials and Methods: Forty SPF chickens was divided into 2 groups. Differential transcriptional profile in the infected SPF chickens trachea tissue was compared to those of control group in the early stage of infection by Illumina RNA-seq technique paired-end and strand-specific sequencing. Differentially expressed genes (DEGs) of transcriptome profiling of the trachea from the infected group were identified. Gene ontology category, KEGG pathway, and STRING analysis were analyzed to identify relationships among differentially expressed genes. Results: Twenty-eight apoptotic genes were identified. They consisted of six pathways related to cell death: the extrinsic pathway, intrinsic pathway, endoplasmic reticulum stress pathway, MAPK signaling pathway, and cell death by NFkB and activates mTOR pathway and some regulator and apoptosis inhibitors. Conclusion: All of the apoptotic genes in our study were up-regulated. Among these genes, the more fold change value was for TRADD and BCL2A1 genes, and the less fold change value was for MAP3K14, NFKB1, PIK3CB, and ITPR2 genes.https://ijm.tums.ac.ir/index.php/ijm/article/view/2847chickens;infectious bronchitis virus;escherichia coli;apoptosis;rna sequencing |
| spellingShingle | Shabnam Hashemi Seyed Masoud Hosseini Arash Ghalyanchilangeroudi Nariman Sheikhi Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic Escherichia coli Iranian Journal of Microbiology chickens; infectious bronchitis virus; escherichia coli; apoptosis; rna sequencing |
| title | Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic Escherichia coli |
| title_full | Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic Escherichia coli |
| title_fullStr | Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic Escherichia coli |
| title_full_unstemmed | Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic Escherichia coli |
| title_short | Transcriptome based analysis of apoptosis genes in chickens co-infected with avian infectious bronchitis virus and pathogenic Escherichia coli |
| title_sort | transcriptome based analysis of apoptosis genes in chickens co infected with avian infectious bronchitis virus and pathogenic escherichia coli |
| topic | chickens; infectious bronchitis virus; escherichia coli; apoptosis; rna sequencing |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/2847 |
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