Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor
Abstract The imperfect denitrifier, Candidatus (Ca.) Desulfobacillus denitrificans, which lacks nitric oxide (NO) reductase, frequently appears in anammox bioreactors depending on the operating conditions. We used genomic and metatranscriptomic analyses to evaluate the metabolic potential of Ca. D....
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| Format: | Article |
| Language: | English |
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Wiley
2021-08-01
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| Series: | MicrobiologyOpen |
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| Online Access: | https://doi.org/10.1002/mbo3.1227 |
| _version_ | 1818733911566450688 |
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| author | Takashi Okubo Hideto Takami |
| author_facet | Takashi Okubo Hideto Takami |
| author_sort | Takashi Okubo |
| collection | DOAJ |
| description | Abstract The imperfect denitrifier, Candidatus (Ca.) Desulfobacillus denitrificans, which lacks nitric oxide (NO) reductase, frequently appears in anammox bioreactors depending on the operating conditions. We used genomic and metatranscriptomic analyses to evaluate the metabolic potential of Ca. D. denitrificans and deduce its functional relationships to anammox bacteria (i.e., Ca. Brocadia pituitae). Although Ca. D. denitrificans is hypothesized to supply NO to Ca. B. pituitae as a byproduct of imperfect denitrification, this microbe also possesses hydroxylamine oxidoreductase, which catalyzes the oxidation of hydroxylamine to NO and potentially the reverse reaction. Ca. D. denitrificans can use a range of electron donors for denitrification, including aromatic compounds, glucose, sulfur compounds, and hydrogen, but metatranscriptomic analysis suggested that the major electron donors are aromatic compounds, which inhibit anammox activity. The interrelationship between Ca. D. denitirificans and Ca. B. pituitae via the metabolism of aromatic compounds may govern the population balance of both species. Ca. D. denitrificans also has the potential to fix CO2 via an irregular Calvin cycle and couple denitrification to the oxidation of hydrogen and sulfur compounds under chemolithoautotrophic conditions. This metabolic versatility, which suggests a mixotrophic lifestyle, would facilitate the growth of Ca. D. denitrificans in the anammox bioreactor. |
| first_indexed | 2024-12-17T23:56:59Z |
| format | Article |
| id | doaj.art-ae04fadf283a4fb59487ac9f558a274f |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-12-17T23:56:59Z |
| publishDate | 2021-08-01 |
| publisher | Wiley |
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| spelling | doaj.art-ae04fadf283a4fb59487ac9f558a274f2022-12-21T21:28:02ZengWileyMicrobiologyOpen2045-88272021-08-01104n/an/a10.1002/mbo3.1227Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactorTakashi Okubo0Hideto Takami1Marine Microbiology, Atmosphere and Ocean Research Institute The University of Tokyo Kashiwa JapanMarine Microbiology, Atmosphere and Ocean Research Institute The University of Tokyo Kashiwa JapanAbstract The imperfect denitrifier, Candidatus (Ca.) Desulfobacillus denitrificans, which lacks nitric oxide (NO) reductase, frequently appears in anammox bioreactors depending on the operating conditions. We used genomic and metatranscriptomic analyses to evaluate the metabolic potential of Ca. D. denitrificans and deduce its functional relationships to anammox bacteria (i.e., Ca. Brocadia pituitae). Although Ca. D. denitrificans is hypothesized to supply NO to Ca. B. pituitae as a byproduct of imperfect denitrification, this microbe also possesses hydroxylamine oxidoreductase, which catalyzes the oxidation of hydroxylamine to NO and potentially the reverse reaction. Ca. D. denitrificans can use a range of electron donors for denitrification, including aromatic compounds, glucose, sulfur compounds, and hydrogen, but metatranscriptomic analysis suggested that the major electron donors are aromatic compounds, which inhibit anammox activity. The interrelationship between Ca. D. denitirificans and Ca. B. pituitae via the metabolism of aromatic compounds may govern the population balance of both species. Ca. D. denitrificans also has the potential to fix CO2 via an irregular Calvin cycle and couple denitrification to the oxidation of hydrogen and sulfur compounds under chemolithoautotrophic conditions. This metabolic versatility, which suggests a mixotrophic lifestyle, would facilitate the growth of Ca. D. denitrificans in the anammox bioreactor.https://doi.org/10.1002/mbo3.1227anammox bioreactorCa. Desulfobacillus denitrificansdenitrificationmetabolic pathway |
| spellingShingle | Takashi Okubo Hideto Takami Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor MicrobiologyOpen anammox bioreactor Ca. Desulfobacillus denitrificans denitrification metabolic pathway |
| title | Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor |
| title_full | Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor |
| title_fullStr | Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor |
| title_full_unstemmed | Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor |
| title_short | Metabolic potential of the imperfect denitrifier Candidatus Desulfobacillus denitrificans in an anammox bioreactor |
| title_sort | metabolic potential of the imperfect denitrifier candidatus desulfobacillus denitrificans in an anammox bioreactor |
| topic | anammox bioreactor Ca. Desulfobacillus denitrificans denitrification metabolic pathway |
| url | https://doi.org/10.1002/mbo3.1227 |
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