The Proteome of the Murine Presynaptic Active Zone
The proteome of the presynaptic active zone controls neurotransmitter release and the short- and long-term structural and functional dynamics of the nerve terminal. The proteinaceous inventory of the presynaptic active zone has recently been reported. This review will evaluate the subcellular fracti...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-04-01
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| Series: | Proteomes |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2227-7382/2/2/243 |
| _version_ | 1798004049183768576 |
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| author | Melanie Laßek Jens Weingarten Walter Volknandt |
| author_facet | Melanie Laßek Jens Weingarten Walter Volknandt |
| author_sort | Melanie Laßek |
| collection | DOAJ |
| description | The proteome of the presynaptic active zone controls neurotransmitter release and the short- and long-term structural and functional dynamics of the nerve terminal. The proteinaceous inventory of the presynaptic active zone has recently been reported. This review will evaluate the subcellular fractionation protocols and the proteomic approaches employed. A breakthrough for the identification of the proteome of the presynaptic active zone was the successful employment of antibodies directed against a cytosolic epitope of membrane integral synaptic vesicle proteins for the immunopurification of synaptic vesicles docked to the presynaptic plasma membrane. Combining immunopurification and subsequent analytical mass spectrometry, hundreds of proteins, including synaptic vesicle proteins, components of the presynaptic fusion and retrieval machinery, proteins involved in intracellular and extracellular signaling and a large variety of adhesion molecules, were identified. Numerous proteins regulating the rearrangement of the cytoskeleton are indicative of the functional and structural dynamics of the presynapse. This review will critically discuss both the experimental approaches and prominent protein candidates identified. Many proteins have not previously been assigned to the presynaptic release sites and may be directly involved in the short- and long-term structural modulation of the presynaptic compartment. The identification of proteinaceous constituents of the presynaptic active zone provides the basis for further analyzing the interaction of presynaptic proteins with their targets and opens novel insights into the functional role of these proteins in neuronal communication. |
| first_indexed | 2024-04-11T12:17:17Z |
| format | Article |
| id | doaj.art-ae06447567c24c70bc4dd93bda1fbf81 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-11T12:17:17Z |
| publishDate | 2014-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-ae06447567c24c70bc4dd93bda1fbf812022-12-22T04:24:15ZengMDPI AGProteomes2227-73822014-04-012224325710.3390/proteomes2020243proteomes2020243The Proteome of the Murine Presynaptic Active ZoneMelanie Laßek0Jens Weingarten1Walter Volknandt2Institute for Cell Biology and Neuroscience, Department Molecular and Cellular Neurobiology, Max von Laue Str. 13, 60438 Frankfurt am Main, GermanyInstitute for Cell Biology and Neuroscience, Department Molecular and Cellular Neurobiology, Max von Laue Str. 13, 60438 Frankfurt am Main, GermanyInstitute for Cell Biology and Neuroscience, Department Molecular and Cellular Neurobiology, Max von Laue Str. 13, 60438 Frankfurt am Main, GermanyThe proteome of the presynaptic active zone controls neurotransmitter release and the short- and long-term structural and functional dynamics of the nerve terminal. The proteinaceous inventory of the presynaptic active zone has recently been reported. This review will evaluate the subcellular fractionation protocols and the proteomic approaches employed. A breakthrough for the identification of the proteome of the presynaptic active zone was the successful employment of antibodies directed against a cytosolic epitope of membrane integral synaptic vesicle proteins for the immunopurification of synaptic vesicles docked to the presynaptic plasma membrane. Combining immunopurification and subsequent analytical mass spectrometry, hundreds of proteins, including synaptic vesicle proteins, components of the presynaptic fusion and retrieval machinery, proteins involved in intracellular and extracellular signaling and a large variety of adhesion molecules, were identified. Numerous proteins regulating the rearrangement of the cytoskeleton are indicative of the functional and structural dynamics of the presynapse. This review will critically discuss both the experimental approaches and prominent protein candidates identified. Many proteins have not previously been assigned to the presynaptic release sites and may be directly involved in the short- and long-term structural modulation of the presynaptic compartment. The identification of proteinaceous constituents of the presynaptic active zone provides the basis for further analyzing the interaction of presynaptic proteins with their targets and opens novel insights into the functional role of these proteins in neuronal communication.http://www.mdpi.com/2227-7382/2/2/243presynaptic active zoneproteome |
| spellingShingle | Melanie Laßek Jens Weingarten Walter Volknandt The Proteome of the Murine Presynaptic Active Zone Proteomes presynaptic active zone proteome |
| title | The Proteome of the Murine Presynaptic Active Zone |
| title_full | The Proteome of the Murine Presynaptic Active Zone |
| title_fullStr | The Proteome of the Murine Presynaptic Active Zone |
| title_full_unstemmed | The Proteome of the Murine Presynaptic Active Zone |
| title_short | The Proteome of the Murine Presynaptic Active Zone |
| title_sort | proteome of the murine presynaptic active zone |
| topic | presynaptic active zone proteome |
| url | http://www.mdpi.com/2227-7382/2/2/243 |
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