kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite
Background: Cutaneous Leishmaniasis, is endemically observed in different parts of Iran in two forms of anthroponotic and zoonotic. The identification of both species and the type of disease are beneficial for treatment and prevention. Microscopic identification of Leishmania species has not provide...
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| Format: | Article |
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Kerman University of Medical Sciences
2019-01-01
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| Series: | Journal of Kerman University of Medical Sciences |
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| Online Access: | https://jkmu.kmu.ac.ir/article_87269_dc2363c8fefc8079b06cae288da48912.pdf |
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| author | Hadi Mirahmadi Ahmad Mehravaran Nasrin Rezaee Saber Gholizadeh Saber Raeghi Elham-Sadat Roointan |
| author_facet | Hadi Mirahmadi Ahmad Mehravaran Nasrin Rezaee Saber Gholizadeh Saber Raeghi Elham-Sadat Roointan |
| author_sort | Hadi Mirahmadi |
| collection | DOAJ |
| description | Background: Cutaneous Leishmaniasis, is endemically observed in different parts of Iran in two forms of anthroponotic and zoonotic. The identification of both species and the type of disease are beneficial for treatment and prevention. Microscopic identification of Leishmania species has not provided promising efficacy. The aim of this study was to determine the Leishmania species that are responsible for cutaneous leishmaniasis in Zahedan/ Iran by using PCR and PCR-RFLP techniques. Method: Direct smears were obtained from cutaneous leishmaniasis suspected individuals with low parasitemia in cutaneous lesions referred to Zahedan health centers. Eventually, the DNA was extracted from smears using DNA extraction kit. PCR was used to amplify both Leishmania kinetoplastic DNA (kDNA) and ITS1 locus of ribosomal DNA. Additionally, PCR-RFLP on ITS1 products was conducted to determine parasite species. Results: PCR-RFLP test (detecting ITS1 locus) on all positive samples in microscopic analysis led to the identification of Leishmania major in 52 samples (54.7%), and 43 cases were detected to have Leishmania tropica (45.3%). On the other hand, kDNA-PCR results indicated a frequency of 68 (55.7%) for L. major and 54 (44.3%) for L. tropica. Conclusion: Due to the high frequency of kDNA in parasitic genome, PCR-kDNA compared to PCR-RFLP shows a higher efficiency and accuracy not only in identifying infection, but also in determining parasite species, especially among the patients with fewer lesions. This study also indicates that both L. tropica and L. major could be found in Zahedan, with a greater tropical leishmaniasis endemicity. |
| first_indexed | 2024-03-13T02:09:23Z |
| format | Article |
| id | doaj.art-ae083f18bc9042ca8215a483e7fb47ca |
| institution | Directory Open Access Journal |
| issn | 2008-2843 |
| language | English |
| last_indexed | 2024-03-13T02:09:23Z |
| publishDate | 2019-01-01 |
| publisher | Kerman University of Medical Sciences |
| record_format | Article |
| series | Journal of Kerman University of Medical Sciences |
| spelling | doaj.art-ae083f18bc9042ca8215a483e7fb47ca2023-07-01T05:16:06ZengKerman University of Medical SciencesJournal of Kerman University of Medical Sciences2008-28432019-01-0126111110.22062/jkmu.2019.8726987269kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of ParasiteHadi Mirahmadi0Ahmad Mehravaran1Nasrin Rezaee2Saber Gholizadeh3Saber Raeghi4Elham-Sadat Roointan5Assistant Professor, Infectious Diseases and Tropical Medicine Research Center, Tuberculosis Institute & Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, IranAssistant Professor, Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, IranInstructor, Infectious Diseases and Tropical Medicine Research Center, Tuberculosis Institute & Department of Parasitology and Mycology, Faculty of Medicine, Zahedan University of Medical Sciences, Zahedan, IranAssociate Professor, Department of Medical Entomology, School of Public Health, Urmia University of Medical Sciences, Urmia, IranAssistant Professor, Department of Laboratory Sciences, Maragheh University of Medical Sciences, Maragheh, IranAssistant Professor, Department of Parasitology, Shahid Beheshti University of Medical Sciences, Tehran, IranBackground: Cutaneous Leishmaniasis, is endemically observed in different parts of Iran in two forms of anthroponotic and zoonotic. The identification of both species and the type of disease are beneficial for treatment and prevention. Microscopic identification of Leishmania species has not provided promising efficacy. The aim of this study was to determine the Leishmania species that are responsible for cutaneous leishmaniasis in Zahedan/ Iran by using PCR and PCR-RFLP techniques. Method: Direct smears were obtained from cutaneous leishmaniasis suspected individuals with low parasitemia in cutaneous lesions referred to Zahedan health centers. Eventually, the DNA was extracted from smears using DNA extraction kit. PCR was used to amplify both Leishmania kinetoplastic DNA (kDNA) and ITS1 locus of ribosomal DNA. Additionally, PCR-RFLP on ITS1 products was conducted to determine parasite species. Results: PCR-RFLP test (detecting ITS1 locus) on all positive samples in microscopic analysis led to the identification of Leishmania major in 52 samples (54.7%), and 43 cases were detected to have Leishmania tropica (45.3%). On the other hand, kDNA-PCR results indicated a frequency of 68 (55.7%) for L. major and 54 (44.3%) for L. tropica. Conclusion: Due to the high frequency of kDNA in parasitic genome, PCR-kDNA compared to PCR-RFLP shows a higher efficiency and accuracy not only in identifying infection, but also in determining parasite species, especially among the patients with fewer lesions. This study also indicates that both L. tropica and L. major could be found in Zahedan, with a greater tropical leishmaniasis endemicity.https://jkmu.kmu.ac.ir/article_87269_dc2363c8fefc8079b06cae288da48912.pdfleishmania tropicaleishmania majorzahedanpcr-rflpits1kdna |
| spellingShingle | Hadi Mirahmadi Ahmad Mehravaran Nasrin Rezaee Saber Gholizadeh Saber Raeghi Elham-Sadat Roointan kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite Journal of Kerman University of Medical Sciences leishmania tropica leishmania major zahedan pcr-rflp its1 kdna |
| title | kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite |
| title_full | kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite |
| title_fullStr | kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite |
| title_full_unstemmed | kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite |
| title_short | kDNA and Molecular Typing of Leishmania spp. of Cutaneous Leishmaniasis Patients in Sistan and Baluchestan Province with Low Amount of Parasite |
| title_sort | kdna and molecular typing of leishmania spp of cutaneous leishmaniasis patients in sistan and baluchestan province with low amount of parasite |
| topic | leishmania tropica leishmania major zahedan pcr-rflp its1 kdna |
| url | https://jkmu.kmu.ac.ir/article_87269_dc2363c8fefc8079b06cae288da48912.pdf |
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