Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herds
Accurate and precise differentiation of staphylococci isolated from milk is of importance for udder health management. In particular, the rapid and specific identification of Staphylococcus aureus plays an essential role in the prevention and treatment programs for bovine mastitis. Plasma gelatiniza...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1120305/full |
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| author | Clara Locatelli Clara Locatelli Stefano Gattolin Valentina Monistero Valentina Monistero Bianca Castiglioni Paolo Moroni Paolo Moroni Paolo Moroni Maria Filippa Addis Maria Filippa Addis Paola Cremonesi |
| author_facet | Clara Locatelli Clara Locatelli Stefano Gattolin Valentina Monistero Valentina Monistero Bianca Castiglioni Paolo Moroni Paolo Moroni Paolo Moroni Maria Filippa Addis Maria Filippa Addis Paola Cremonesi |
| author_sort | Clara Locatelli |
| collection | DOAJ |
| description | Accurate and precise differentiation of staphylococci isolated from milk is of importance for udder health management. In particular, the rapid and specific identification of Staphylococcus aureus plays an essential role in the prevention and treatment programs for bovine mastitis. Plasma gelatinization in coagulase assays is routinely used to discriminate S. aureus from other species by detecting the presence of extracellular free staphylocoagulase. However, rarely occurring coagulase-deficient S. aureus strains can be responsible for clinical and subclinical mastitis cases. By investigating S. aureus isolates from a single herd over a 10-year period we identified the persistence of a phenotypically coagulase-negative S. aureus strain and pinpointed the possible cause to a single base pair deletion in the coa gene sequence. Our results support the need to integrate primary biochemical tests with molecular/sequence analysis approaches for correctly identifying and discriminating atypical S. aureus in bovine herds, as the coagulase test alone may fail to detect persistent mastitis-causing strains. |
| first_indexed | 2024-04-09T13:11:33Z |
| format | Article |
| id | doaj.art-ae08d0461cf94b8999e70795a8092b99 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-09T13:11:33Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ae08d0461cf94b8999e70795a8092b992023-05-12T07:04:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11203051120305Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herdsClara Locatelli0Clara Locatelli1Stefano Gattolin2Valentina Monistero3Valentina Monistero4Bianca Castiglioni5Paolo Moroni6Paolo Moroni7Paolo Moroni8Maria Filippa Addis9Maria Filippa Addis10Paola Cremonesi11Department of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Lodi, ItalyLaboratorio di Malattie Infettive degli Animali, Università degli Studi di Milano, Lodi, ItalyItalian National Research Council, Institute of Agricultural Biology and Biotechnology, Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Lodi, ItalyLaboratorio di Malattie Infettive degli Animali, Università degli Studi di Milano, Lodi, ItalyItalian National Research Council, Institute of Agricultural Biology and Biotechnology, Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Lodi, ItalyLaboratorio di Malattie Infettive degli Animali, Università degli Studi di Milano, Lodi, ItalyQuality Milk Production Services, Animal Health Diagnostic Center, Cornell University, Ithaca, NY, United StatesDepartment of Veterinary Medicine and Animal Sciences, Università degli Studi di Milano, Lodi, ItalyLaboratorio di Malattie Infettive degli Animali, Università degli Studi di Milano, Lodi, ItalyItalian National Research Council, Institute of Agricultural Biology and Biotechnology, Lodi, ItalyAccurate and precise differentiation of staphylococci isolated from milk is of importance for udder health management. In particular, the rapid and specific identification of Staphylococcus aureus plays an essential role in the prevention and treatment programs for bovine mastitis. Plasma gelatinization in coagulase assays is routinely used to discriminate S. aureus from other species by detecting the presence of extracellular free staphylocoagulase. However, rarely occurring coagulase-deficient S. aureus strains can be responsible for clinical and subclinical mastitis cases. By investigating S. aureus isolates from a single herd over a 10-year period we identified the persistence of a phenotypically coagulase-negative S. aureus strain and pinpointed the possible cause to a single base pair deletion in the coa gene sequence. Our results support the need to integrate primary biochemical tests with molecular/sequence analysis approaches for correctly identifying and discriminating atypical S. aureus in bovine herds, as the coagulase test alone may fail to detect persistent mastitis-causing strains.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1120305/fullcoagulase genedairy cowgenotypingsequencingStaphylococcus aureus |
| spellingShingle | Clara Locatelli Clara Locatelli Stefano Gattolin Valentina Monistero Valentina Monistero Bianca Castiglioni Paolo Moroni Paolo Moroni Paolo Moroni Maria Filippa Addis Maria Filippa Addis Paola Cremonesi Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herds Frontiers in Microbiology coagulase gene dairy cow genotyping sequencing Staphylococcus aureus |
| title | Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herds |
| title_full | Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herds |
| title_fullStr | Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herds |
| title_full_unstemmed | Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herds |
| title_short | Staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase-negative strains and contribute to their control in dairy cow herds |
| title_sort | staphylococcus aureus coa gene sequence analysis can prevent misidentification of coagulase negative strains and contribute to their control in dairy cow herds |
| topic | coagulase gene dairy cow genotyping sequencing Staphylococcus aureus |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1120305/full |
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