DNA Catalysis: The Chemical Repertoire of DNAzymes
Deoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are obtained by combinatorial in vitro selection methods. Initially conceived to function as gene silencing agents, the scope of DNAzymes has rapidly expanded into diverse fields, including biosensing, diagnostics, logic gat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-11-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/20/11/19730 |
| _version_ | 1818693873609736192 |
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| author | Marcel Hollenstein |
| author_facet | Marcel Hollenstein |
| author_sort | Marcel Hollenstein |
| collection | DOAJ |
| description | Deoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are obtained by combinatorial in vitro selection methods. Initially conceived to function as gene silencing agents, the scope of DNAzymes has rapidly expanded into diverse fields, including biosensing, diagnostics, logic gate operations, and the development of novel synthetic and biological tools. In this review, an overview of all the different chemical reactions catalyzed by DNAzymes is given with an emphasis on RNA cleavage and the use of non-nucleosidic substrates. The use of modified nucleoside triphosphates (dN*TPs) to expand the chemical space to be explored in selection experiments and ultimately to generate DNAzymes with an expanded chemical repertoire is also highlighted. |
| first_indexed | 2024-12-17T13:20:36Z |
| format | Article |
| id | doaj.art-ae0b0a2a4b6b4a25b76e2f45428f2799 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-17T13:20:36Z |
| publishDate | 2015-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-ae0b0a2a4b6b4a25b76e2f45428f27992022-12-21T21:46:53ZengMDPI AGMolecules1420-30492015-11-012011207772080410.3390/molecules201119730molecules201119730DNA Catalysis: The Chemical Repertoire of DNAzymesMarcel Hollenstein0Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, SwitzerlandDeoxyribozymes or DNAzymes are single-stranded catalytic DNA molecules that are obtained by combinatorial in vitro selection methods. Initially conceived to function as gene silencing agents, the scope of DNAzymes has rapidly expanded into diverse fields, including biosensing, diagnostics, logic gate operations, and the development of novel synthetic and biological tools. In this review, an overview of all the different chemical reactions catalyzed by DNAzymes is given with an emphasis on RNA cleavage and the use of non-nucleosidic substrates. The use of modified nucleoside triphosphates (dN*TPs) to expand the chemical space to be explored in selection experiments and ultimately to generate DNAzymes with an expanded chemical repertoire is also highlighted.http://www.mdpi.com/1420-3049/20/11/19730SELEXDNAzymeschemically modified nucleic acidsfunctional nucleic acidsbiosensorstherapeutic nucleic acids |
| spellingShingle | Marcel Hollenstein DNA Catalysis: The Chemical Repertoire of DNAzymes Molecules SELEX DNAzymes chemically modified nucleic acids functional nucleic acids biosensors therapeutic nucleic acids |
| title | DNA Catalysis: The Chemical Repertoire of DNAzymes |
| title_full | DNA Catalysis: The Chemical Repertoire of DNAzymes |
| title_fullStr | DNA Catalysis: The Chemical Repertoire of DNAzymes |
| title_full_unstemmed | DNA Catalysis: The Chemical Repertoire of DNAzymes |
| title_short | DNA Catalysis: The Chemical Repertoire of DNAzymes |
| title_sort | dna catalysis the chemical repertoire of dnazymes |
| topic | SELEX DNAzymes chemically modified nucleic acids functional nucleic acids biosensors therapeutic nucleic acids |
| url | http://www.mdpi.com/1420-3049/20/11/19730 |
| work_keys_str_mv | AT marcelhollenstein dnacatalysisthechemicalrepertoireofdnazymes |